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Bovine monocyte chemotactic protein 1/monocyte chemotactic and activating factor,(MCP-1/CCL2/MCAF)ELISA Kit

Code CSB-E16221B
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Bovine CCL2 ELISA Kit was designed for the quantitative measurement of Bovine CCL2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 78 pg/mL-5000 pg/mL and the sensitivity is 0.039 ng/mL.
Target Name chemokine (C-C motif) ligand 2
Alternative Names CCL2 ELISA Kit; SCYA2C-C motif chemokine 2 ELISA Kit; Acidic seminal fluid protein ELISA Kit; Monocyte chemotactic protein 1A ELISA Kit; MCP-1 ELISA Kit; MCP-1A ELISA Kit; Small-inducible cytokine A2 ELISA Kit
Abbreviation CCL2
Uniprot No. P28291
Species Bos taurus (Bovine)
Sample Types serum, plasma, tissue homogenates
Detection Range 78 pg/mL-5000 pg/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine RBP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)
1:1 Average % 88
Range % 82-95
1:2 Average % 98
Range % 94-102
1:4 Average % 97
Range % 90-105
1:8 Average % 86
Range % 82-92
Recovery
The recovery of bovine RBP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 90 88-96
EDTA plasma (n=4) 100 96-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
10 1.921 1.890 1.906 1.792
5 1.703 1.749 1.726 1.612
2.5 1.345 1.431 1.388 1.274
1.25 1.023 1.075 1.049 0.935
0.625 0.671 0.722 0.697 0.583
0.312 0.429 0.440 0.435 0.321
0.156 0.282 0.289 0.286 0.172
0 0.111 0.116 0.114 　
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs		 ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Acts as a ligand for C-C chemokine receptor CCR2. Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions. Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils. Plays an important role in mediating peripheral nerve injury-induced neuropathic pain. Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B.
Gene References into Functions
  1. CCL2 plays a pivotal role in reproductive tissues and may support maternal-fetal interface to improve efficiency of pregnancy. PMID: 28816360
  2. resence of type 2 diabetes negatively affects the work of cytokines and markers of fibrosis, as evidenced by higher levels of interleukin-1beta and monocyte chemoattractant protein-1, compared with patients without diabetes PMID: 25020170
  3. Studied SNPs in the bovine toll-like receptor 4 (TLR4) and monocyte chemo attractant protein-1(CCL2) genes. PMID: 24813219
  4. The LEP, IGF2 and CCL2 genes showed allelic expression imbalance in liver, kidney and pituitary glands of Polish Holstein-Friesian bulls. PMID: 23184004
  5. immune cells and endothelial cells can interact cooperatively to increase CCL2 secretion in the corpus luteum PMID: 15659710

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Subcellular Location Secreted.
Protein Families Intercrine beta (chemokine CC) family
Database Links

KEGG: bta:281043

STRING: 9913.ENSBTAP00000013146

UniGene: Bt.2408
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