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Bovine monocyte chemotactic protein 1/monocyte chemotactic and activating factor,(MCP-1/CCL2/MCAF)ELISA Kit

Code CSB-E16221B
Size 96T,5×96T,10×96T
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Product Details

Alternative Names
CCL2 ELISA Kit; SCYA2C-C motif chemokine 2 ELISA Kit; Acidic seminal fluid protein ELISA Kit; Monocyte chemotactic protein 1A ELISA Kit; MCP-1 ELISA Kit; MCP-1A ELISA Kit; Small-inducible cytokine A2 ELISA Kit
Abbreviation
CCL2
Uniprot No.
P28291
Species
Bos taurus (Bovine)
Sample Types
serum, plasma, tissue homogenates
Detection Range
78 pg/mL-5000 pg/mL
Sensitivity
0.039 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision

Linearity

Recovery

Typical Data

ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

The Bovine CCL2 ELISA Kit is a tool for the quantification of C-C motif chemokine 2 (CCL2) in bovine serum, plasma, and tissue homogenates. CCL2 is a protein that plays a critical role in immune responses, particularly in the recruitment of immune cells to sites of inflammation. This ELISA kit detects CCL2 with high sensitivity, with a detection range of 78 pg/mL to 5000 pg/mL and a sensitivity of 0.039 ng/mL.

The assay principle is a sandwich measurement, providing quantitative results that can be obtained in as little as 1-5 hours. The kit is optimized for use with a sample volume of 50-100ul, making it a cost-effective and efficient solution for researchers.

CCL2 has been shown to be a reliable biomarker of immune responses. and this kit provides researchers with a precise and reliable method to accurately measure CCL2 levels in bovine samples.

Related Products

CCL2 Proteins

Customer Reviews and Q&A

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Target Background

Function
(From Uniprot)
Acts as a ligand for C-C chemokine receptor CCR2. Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions. Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils. Plays an important role in mediating peripheral nerve injury-induced neuropathic pain. Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B.
Gene References into Functions
  1. CCL2 plays a pivotal role in reproductive tissues and may support maternal-fetal interface to improve efficiency of pregnancy. PMID: 28816360
  2. resence of type 2 diabetes negatively affects the work of cytokines and markers of fibrosis, as evidenced by higher levels of interleukin-1beta and monocyte chemoattractant protein-1, compared with patients without diabetes PMID: 25020170
  3. Studied SNPs in the bovine toll-like receptor 4 (TLR4) and monocyte chemo attractant protein-1(CCL2) genes. PMID: 24813219
  4. The LEP, IGF2 and CCL2 genes showed allelic expression imbalance in liver, kidney and pituitary glands of Polish Holstein-Friesian bulls. PMID: 23184004
  5. immune cells and endothelial cells can interact cooperatively to increase CCL2 secretion in the corpus luteum PMID: 15659710

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Subcellular Location
Secreted.
Protein Families
Intercrine beta (chemokine CC) family
Database Links
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Related pathways
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