Canine Cardiac Troponin Ⅰ(cTn-Ⅰ) ELISA kit

Code CSB-E08772c
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Cardiac Troponin Ⅰ(cTn-Ⅰ)
Alternative Names
TNNI3 ELISA Kit; Troponin I ELISA Kit; cardiac muscle ELISA Kit; Cardiac troponin I ELISA Kit
Abbreviation
cTn-I
Uniprot No.
Species
Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types
serum, plasma, tissue homogenates
Detection Range
23.5 pg/mL-1500 pg/mL
Sensitivity
5.8 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of canine cTn-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %95
Range %90-100
1:2Average %89
Range %85-99
1:4Average %98
Range %91-104
1:8Average %96
Range %91-100
Recovery
The recovery of canine cTn-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9792-108
EDTA plasma (n=4)9590-101
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
15002.245 2.250 2.248 2.071
7501.716 1.704 1.710 1.533
3751.144 1.161 1.153 0.976
187.50.790 0.775 0.783 0.606
940.459 0.474 0.467 0.290
470.305 0.289 0.297 0.120
23.50.215 0.211 0.213 0.036
00.176 0.177 0.177  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Dog cTn-I ELISA Kit was designed for the quantitative measurement of Dog cTn-I protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL.

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Target Background

Function
(From Uniprot)
Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Gene References into Functions
  1. No statistical differences were found between CRP and cTnI levels, either before or after surgical correction of brachycephaly in dogs. PMID: 25673910
  2. The objective of this study was to evaluate the correlation between anatomical components, clinical signs and several biomarkers, used to determine systemic inflammation and myocardial damage in dogs with brachycephalic upper airway obstructive syndrome. PMID: 22937913
  3. The use of NT-pro-BNP and troponin I levels as prognostic indicators for canine heart failure is reported. PMID: 22439324
  4. [NT-proBNP] concentration is significantly higher in dogs with pre-capillary pulmonary hypertension (PH) when compared to dogs with respiratory disease without PH; [NT-proANP] and [cTnI] were not elevated in dogs with pre-capillary PH. PMID: 21835711
  5. Moderate exercise does not present a confounding variable in the interpretation of cardiac tropinin I and C-reactive protein concentrations in normal dogs. PMID: 20840303
  6. Serum troponin I is high in dogs with either precapillary and postcapillary pulmonary hypertension (PH); myocardial damage in dogs with postcapillary PH is likely the consequence of increased mitral valve disease severity. PMID: 20002552
  7. Analysis of troponin I concentration has potential to increase knowledge of overall cardiac remodeling in dogs with mitral valve disease; the effect of age on troponin I levels should be considered in these patients. PMID: 20002554
  8. Circulating cTnI and pro-matrix metallopeptidase 9 increase in proportion to the severity of acute pulmonary thromboembolism PMID: 18047836
  9. The incidence of myocardial injury due to ehrlichiosis, as demonstrated by elevated cardiac troponin I blood levels, is reported. PMID: 18638021

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Protein Families
Troponin I family
Database Links

KEGG: cfa:403566

STRING: 9615.ENSCAFP00000003805

UniGene: Cfa.83

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