Recombinant Dog Troponin I, cardiac muscle (TNNI3)

Code CSB-EP853992DO
MSDS
Size $388
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
TNNI3
Uniprot No.
Research Area
Cardiovascular
Species
Canis lupus familiaris (Dog) (Canis familiaris)
Source
E.coli
Expression Region
2-211aa
Target Protein Sequence
ADESGDAAGCPPPAPAPIRRQSSANYRAYATEPHAKKKSKISASRKLQLKTLMLQIAKQELEREAEERRGEKGRALSTRCQPLELAGLGFAELQDLCRQLHARVDKVDEERYDVEAKVTKNITEIADLTQKIFDLRGKFKRPTLRRVRISADAMMQALLGTRAKESLDLRAHLKQVKKEDTEKENREVGDWRKNIDALSGMEGRKKKFEG
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
31.2 kDa
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Gene References into Functions
  1. No statistical differences were found between CRP and cTnI levels, either before or after surgical correction of brachycephaly in dogs. PMID: 25673910
  2. The objective of this study was to evaluate the correlation between anatomical components, clinical signs and several biomarkers, used to determine systemic inflammation and myocardial damage in dogs with brachycephalic upper airway obstructive syndrome. PMID: 22937913
  3. The use of NT-pro-BNP and troponin I levels as prognostic indicators for canine heart failure is reported. PMID: 22439324
  4. [NT-proBNP] concentration is significantly higher in dogs with pre-capillary pulmonary hypertension (PH) when compared to dogs with respiratory disease without PH; [NT-proANP] and [cTnI] were not elevated in dogs with pre-capillary PH. PMID: 21835711
  5. Moderate exercise does not present a confounding variable in the interpretation of cardiac tropinin I and C-reactive protein concentrations in normal dogs. PMID: 20840303
  6. Serum troponin I is high in dogs with either precapillary and postcapillary pulmonary hypertension (PH); myocardial damage in dogs with postcapillary PH is likely the consequence of increased mitral valve disease severity. PMID: 20002552
  7. Analysis of troponin I concentration has potential to increase knowledge of overall cardiac remodeling in dogs with mitral valve disease; the effect of age on troponin I levels should be considered in these patients. PMID: 20002554
  8. Circulating cTnI and pro-matrix metallopeptidase 9 increase in proportion to the severity of acute pulmonary thromboembolism PMID: 18047836
  9. The incidence of myocardial injury due to ehrlichiosis, as demonstrated by elevated cardiac troponin I blood levels, is reported. PMID: 18638021

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Protein Families
Troponin I family
Database Links

KEGG: cfa:403566

STRING: 9615.ENSCAFP00000003805

UniGene: Cfa.83

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