Fish Superoxide Dismutase(SOD)ELISA kit

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Fish Superoxide Dismutase(SOD)ELISA kit

Name: Fish Superoxide Dismutase(SOD)ELISA kit
Brand: CUSABIO
SKU: CSB-E15929Fh

^Instructions

Code	CSB-E15929Fh
Size	96T,5×96T,10×96T
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Target Data

Target Name

Superoxide Dismutase(SOD)ELISA kit

Alternative Names

sod1 ELISA Kit; cuzn ELISA Kit; Superoxide dismutase [Cu-Zn] ELISA Kit; EC 1.15.1.1 ELISA Kit

Abbreviation

SOD

Uniprot No.

O73872

Species

Fish

Sample Types

serum, plasma, tissue homogenates

Detection Range

1.25 ng/mL-20 ng/mL

Sensitivity

0.625 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of fish SOD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	95
	Range %	92-98
1:200	Average %	103
	Range %	100-106
1:400	Average %	98
	Range %	94-102
1:800	Average %	92
	Range %	88-96

Recovery

The recovery of fish SOD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	96-103
EDTA plasma (n=4)	89	85-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
20	0.092	0.099	0.096
10	0.143	0.152	0.148
5	0.245	0.223	0.234
2.5	0.588	0.575	0.582
1.25	1.397	1.402	1.400
0	2.214	2.352	2.283

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Dietary sodium butyrate ameliorated the blood stress biomarkers, heat shock proteins, and immune response of Nile tilapia (Oreochromis niloticus) exposed to heat … MAO Dawood,Journal of Thermal Biology,2020
The potential role of nano-selenium and vitamin C on the performances of Nile tilapia (Oreochromis niloticus) Dawood MAO,Environ Sci Pollut Res Int,2020
Vaccination of fish against Aeromonas hydrophila infections using the novel approach of transcutaneous immunization with dissolving microneedle patches in aquaculture Yun S, et al,Fish & Shellfish Immunology,2019
Probiotic effects of Aspergillus oryzae on the oxidative status, heat shock protein, and immune related gene expression of Nile tilapia (Oreochromis niloticus) under hypoxia challenge Dawood M A O, et al,Aquaculture,2019
The evaluation of growth performance, blood health, oxidative status and immune-related gene expression in Nile tilapia (Oreochromis niloticus) fed dietary nanoselenium spheres produced by lactic acid bacteria Dawood M A O, et al,Aquaculture,2019
Synergistic Effects of Selenium Nanoparticles and Vitamin E on Growth, Immune-Related Gene Expression, and Regulation of Antioxidant Status of Nile Tilapia (Oreochromis niloticus) Dawood MAO, et al,Biological Trace Element Research,2019
Effects of UV Radiation on Oxidative Stress in Yellowtail Clownfish Amphiprion clarkii Han Seok Ryu,Ocean Science Journal,2019
Synbiotic Effects of Aspergillus oryzae and β-Glucan on Growth and Oxidative and Immune Responses of Nile Tilapia, Oreochromis niloticus Dawood MAO, et al,Probiotics and Antimicrobial Proteins,2019
Physiological effects of various light spectra on oxidative stress by starvation in olive flounder, Paralichthys olivaceus Cheol Young Choi.et al,Molecular & Cellular Toxicology,2018
Effects of waterborne copper on oxidative stress and immune responses in red seabream, Pagrus major Cheol Young Choi.et al,Molecular & Cellular Toxicology,2018
Negative effect of chronic cadmium exposure on growth, histology, ultrastructure, antioxidant and innate immune responses in the liver of zebrafish: Preventive role Shuang-Shuang Yuan.et al,Ecotoxicology and Environmental Safety,2017
Effect of different wavelengths of light on the antioxidant and immunity status of juvenile rock bream, Oplegnathus fasciatus, exposed to thermal stress Jong Ryeol Choe.et al,Ocean Science Journal,2017
Effects of melatonin injection or green-wavelength LED light on the antioxidant system in goldfish (Carassius auratus) during thermal stress Seo JinJung.et al,Fish & Shellfish Immunology,2016
Effects of different light wavelengths from LEDs on oxidative stress and apoptosis in olive flounder (Paralichthys olivaceus) at high water temperatures. Kim BS.et al,Fish Shellfish Immunol.,2016
The effect of green and red light spectra and their intensity on the oxidative stress and non-specific immune responses in gold-striped amberjack, Seriola lalandi Choi Y J.et al,Marine and Freshwater Behaviour and Physiology,2016

■ Q&A

I use the SOD kit(fish) after centrifuge the standard vial at 6000~10000rpm for 30s before opening 2:56 pm
i must pipetting standard vial or not?

Thanks for your inquiry. The standard in this kit is 10 times concentrated and it requires dilutions.For the specific cooperations,pls refer to the "Standard" part stated on page 9 of the manual.I send the manual to you by e-mail. Pls let me know if you have any further questions. Thank you.

■ Customer Reviews

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Function	Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
Gene References into Functions	fenofibrate almost completely abolished GM-induced reactive oxygen species generation, which seemed to be mediated at least in part by the restoration of the expression of PPARalphadependent antioxidant enzymes, including catalase and superoxide dismutase (SOD)-1. PMID: 28290603 The earliest event in the pathophysiology of amyotrohic lateral sclerosis in the mutant sod1 zebrafish model involves neuronal stress in inhibitory interneurons, resulting from mutant Sod1 expression. PMID: 23281025 A hierarchic gene expression of copper homeostatic genes was demonstrated between atp7a, sp1 and sod1 in zebrafish. PMID: 21167140 depresses cathepsin L activity stimulated by free radicals and prevents otic complications associated with bone erosion PMID: 11771045 This isoform, when expressed in PC-12 cells affects t-butylhydroperoxide-induced apoptosis differentially from its isoenzyme. PMID: 12551919 Copper/zinc superoxide dismutase was cloned from the zebrafish ( Danio rerio). Evidence is presented that SOD protects against paraquat toxicity in fish. PMID: 12876653 Glia maturation factor-null cells ahow a concurrent decrease in CuZnSOD astrocytes. PMID: 15260123 Show More Hide All
Subcellular Location	Cytoplasm, Nucleus
Protein Families	Cu-Zn superoxide dismutase family
Database Links	KEGG: dre:30553 STRING: 7955.ENSDARP00000064375 UniGene: Dr.75822

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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