Fish Superoxide Dismutase(SOD)ELISA kit

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Fish Superoxide Dismutase(SOD)ELISA kit

Specification
Target

Code

CSB-E15929Fh

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Product Type

ELISA Kit

Size

96T,5×96T,10×96T

Uniprot No.

O73872

Lead Time

3-5 working days

Abbreviation

SOD

Target Name

Superoxide Dismutase(SOD)ELISA kit

Species

Fish

Sample Types

serum, plasma, tissue homogenates

Detection Range

1.25 ng/mL-20 ng/mL

Sensitivity

0.625 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Protocol

Instructions

Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of fish SOD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	95
	Range %	92-98
1:200	Average %	103
	Range %	100-106
1:400	Average %	98
	Range %	94-102
1:800	Average %	92
	Range %	88-96

Recovery

The recovery of fish SOD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	96-103
EDTA plasma (n=4)	89	85-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
20	0.092	0.099	0.096
10	0.143	0.152	0.148
5	0.245	0.223	0.234
2.5	0.588	0.575	0.582
1.25	1.397	1.402	1.400
0	2.214	2.352	2.283

References

The effect of green and red light spectra and their intensity on the oxidative stress and non-specific immune responses in gold-striped amberjack, Seriola lalandi Choi Y J.et al,Marine and Freshwater Behaviour and Physiology,1-12.
Effects of different light wavelengths from LEDs on oxidative stress and apoptosis in olive flounder (Paralichthys olivaceus) at high water temperatures. Kim BS.et al,Fish Shellfish Immunol.,55:460-468
Effects of melatonin injection or green-wavelength LED light on the antioxidant system in goldfish (Carassius auratus) during thermal stress Seo JinJung.et al,Fish & Shellfish Immunology,2016 52:157-166
Effect of different wavelengths of light on the antioxidant and immunity status of juvenile rock bream, Oplegnathus fasciatus, exposed to thermal stress Jong Ryeol Choe.et al,Ocean Science Journal,Sep 7
Negative effect of chronic cadmium exposure on growth, histology, ultrastructure, antioxidant and innate immune responses in the liver of zebrafish: Preventive role Shuang-Shuang Yuan.et al,Ecotoxicology and Environmental Safety,May
Effects of waterborne copper on oxidative stress and immune responses in red seabream, Pagrus major Cheol Young Choi.et al,Molecular & Cellular Toxicology,June 23
Physiological effects of various light spectra on oxidative stress by starvation in olive flounder, Paralichthys olivaceus Cheol Young Choi.et al,Molecular & Cellular Toxicology,September 25
Synbiotic Effects of Aspergillus oryzae and β-Glucan on Growth and Oxidative and Immune Responses of Nile Tilapia, Oreochromis niloticus Dawood MAO, et al,Probiotics and Antimicrobial Proteins,January 8

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Function	Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
Subcellular Location	Cytoplasm, Nucleus
Protein Families	Cu-Zn superoxide dismutase family
Database Links	KEGG: dre:30553 STRING: 7955.ENSDARP00000064375 UniGene: Dr.75822

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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