Fish Superoxide Dismutase(SOD)ELISA kit

Instructions
Code CSB-E15929Fh
Size 96T,5×96T,10×96T
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Product Details

Target Name Superoxide Dismutase(SOD)ELISA kit
Alternative Names sod1 ELISA Kit; cuzn ELISA Kit; Superoxide dismutase [Cu-Zn] ELISA Kit; EC 1.15.1.1 ELISA Kit
Abbreviation SOD
Uniprot No. O73872
Species Fish
Sample Types serum, plasma, tissue homogenates
Detection Range 1.25 ng/mL-20 ng/mL
Sensitivity 0.625 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of fish SOD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 95  
Range % 92-98  
1:200 Average % 103  
Range % 100-106  
1:400 Average % 98  
Range % 94-102  
1:800 Average % 92  
Range % 88-96  
Recovery
The recovery of fish SOD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 99 96-103  
EDTA plasma (n=4) 89 85-93  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
20 0.092 0.099 0.096    
10 0.143 0.152 0.148    
5 0.245 0.223 0.234    
2.5 0.588 0.575 0.582    
1.25 1.397 1.402 1.400    
0 2.214 2.352 2.283    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Q&A and Customer Reviews

 Q&A
Q:

I use the SOD kit(fish) after centrifuge the standard vial at 6000~10000rpm for 30s before opening 2:56 pm
i must pipetting standard vial or not?

A:
Thanks for your inquiry. The standard in this kit is 10 times concentrated and it requires dilutions.For the specific cooperations,pls refer to the "Standard" part stated on page 9 of the manual.I send the manual to you by e-mail. Pls let me know if you have any further questions. Thank you.
 Customer Reviews

Target Data

Function Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
Gene References into Functions
  1. fenofibrate almost completely abolished GM-induced reactive oxygen species generation, which seemed to be mediated at least in part by the restoration of the expression of PPARalphadependent antioxidant enzymes, including catalase and superoxide dismutase (SOD)-1. PMID: 28290603
  2. The earliest event in the pathophysiology of amyotrohic lateral sclerosis in the mutant sod1 zebrafish model involves neuronal stress in inhibitory interneurons, resulting from mutant Sod1 expression. PMID: 23281025
  3. A hierarchic gene expression of copper homeostatic genes was demonstrated between atp7a, sp1 and sod1 in zebrafish. PMID: 21167140
  4. depresses cathepsin L activity stimulated by free radicals and prevents otic complications associated with bone erosion PMID: 11771045
  5. This isoform, when expressed in PC-12 cells affects t-butylhydroperoxide-induced apoptosis differentially from its isoenzyme. PMID: 12551919
  6. Copper/zinc superoxide dismutase was cloned from the zebrafish ( Danio rerio). Evidence is presented that SOD protects against paraquat toxicity in fish. PMID: 12876653
  7. Glia maturation factor-null cells ahow a concurrent decrease in CuZnSOD astrocytes. PMID: 15260123

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Subcellular Location Cytoplasm, Nucleus
Protein Families Cu-Zn superoxide dismutase family
Database Links

KEGG: dre:30553

STRING: 7955.ENSDARP00000064375

UniGene: Dr.75822

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