Goat Immunoglobulin A(IgA) ELISA Kit

Code CSB-E13135G
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation IgA
Target Name Immunoglobulin A(IgA)
Species Capra hircus (Goat)
Sample Types serum, plasma
Detection Range 29 ng/mL-7500 ng/mL
Sensitivity 29 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of goat immunoglobulin A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:5000 Average % 94  
Range % 90-103  
1:10000 Average % 96  
Range % 92-100  
1:20000 Average % 90  
Range % 85-97  
1:40000 Average % 92  
Range % 85-103  
Recovery
The recovery of goat immunoglobulin A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 90-100  
EDTA plasma (n=4) 95 91-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
7500 0.289 0.312 0.301    
1875 0.558 0.542 0.550    
468 0.912 0.921 0.917    
117 1.612 1.543 1.578    
29 1.853 1.934 1.894    
0 2.406 2.398 2.402    
             
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