Goat Immunoglobulin A(IgA) ELISA Kit

Instructions
Code CSB-E13135G
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name Immunoglobulin A(IgA)
Abbreviation IgA
Species Capra hircus (Goat)
Sample Types serum, plasma
Detection Range 29 ng/mL-7500 ng/mL
Sensitivity 29 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of goat immunoglobulin A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:5000 Average % 94  
Range % 90-103  
1:10000 Average % 96  
Range % 92-100  
1:20000 Average % 90  
Range % 85-97  
1:40000 Average % 92  
Range % 85-103  
Recovery
The recovery of goat immunoglobulin A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 90-100  
EDTA plasma (n=4) 95 91-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
7500 0.289 0.312 0.301    
1875 0.558 0.542 0.550    
468 0.912 0.921 0.917    
117 1.612 1.543 1.578    
29 1.853 1.934 1.894    
0 2.406 2.398 2.402    
             
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
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