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Goat interleukin 12,IL-12/P40 ELISA KIT

Citations (1) Protocol
Code CSB-E12103G
Size 96T,5×96T,10×96T
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Product Details

Alternative Names
IL12B ELISA Kit; Interleukin-12 subunit beta ELISA Kit; IL-12B ELISA Kit; Cytotoxic lymphocyte maturation factor 40 kDa subunit ELISA Kit; CLMF p40 ELISA Kit; IL-12 subunit p40 ELISA Kit
Abbreviation
IL12B
Uniprot No.
P68221
Species
Capra hircus (Goat)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
9.37 pg/mL-600 pg/mL
Sensitivity
2.34 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of goat IL-12/P40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 95  
Range % 89-102  
1:2 Average % 86  
Range % 82-97  
1:4 Average % 93  
Range % 85-102  
1:8 Average % 95  
Range % 89-105  
Recovery
The recovery of goat IL-12/P40 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 85 81-90  
EDTA plasma (n=4) 92 87-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
600 1.974 1.942 1.958 1.857  
300 1.331 1.362 1.347 1.246  
150 0.912 0.924 0.918 0.817  
75 0.587 0.612 0.600 0.499  
37.5 0.401 0.388 0.395 0.294  
18.75 0.295 0.309 0.302 0.201  
9.37 0.214 0.225 0.220 0.119  
0 0.102 0.100 0.101    
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

This Goat IL12B ELISA Kit was designed for the quantitative measurement of Goat IL12B protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 9.38 pg/mL-600 pg/mL and the sensitivity is 2.34 pg/mL.

Citations

Related Products

IL12B Proteins

Customer Reviews and Q&A

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Target Background

Function
(From Uniprot)
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.; Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
Subcellular Location
Secreted.
Protein Families
Type I cytokine receptor family, Type 3 subfamily
Database Links

KEGG: chx:100860845

UniGene: Chi.38710

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