Sheep Immunoglobulin E,IgE ELISA Kit

Code CSB-E13683Sh
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation IgE
Target Name Immunoglobulin E,IgE
Species Ovis aries (Sheep)
Sample Types serum, plasma, tissue homogenates
Detection Range 125 ng/mL-8000 ng/mL
Sensitivity 31.25 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of sheep IgE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %86
Range %80-94
1:2Average %97
Range %92-100
1:4Average %90
Range %85-95
1:8Average %95
Range %87-99
Recovery
The recovery of sheep IgE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9789-102
EDTA plasma (n=4)9691-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
80002.632 2.512 2.572 2.428
40002.074 1.974 2.024 1.880
20001.371 1.271 1.321 1.177
10000.815 0.805 0.810 0.666
5000.470 0.460 0.465 0.321
2500.348 0.338 0.343 0.199
1250.238 0.229 0.234 0.090
00.144 0.143 0.144  
References
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