Guinea pig Immunoglobulin G,IgG ELISA Kit

Code CSB-E10125Gu
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Immunoglobulin G,IgG
Abbreviation
IgG
Species
Cavia porcellus (Guinea pig)
Sample Types
serum, plasma
Detection Range
62.5 μg/mL-1000 μg/mL
Sensitivity
62.5 μg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IgG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:10Average %99
Range %95-103
1:20Average %94
Range %91-98
1:40Average %95
Range %90-100
1:80Average %92
Range %85-96
Recovery
The recovery of guinea pig IgG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9792-104
EDTA plasma (n=4)9684-110
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/mlOD1OD2Average
10000.139 0.141 0.140
5000.300 0.312 0.306
2500.600 0.604 0.602
1250.869 0.872 0.871
62.51.372 1.395 1.384
02.916 2.709 2.813
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Guinea pig IgG ELISA Kit was designed for the quantitative measurement of Guinea pig IgG protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 62.5 μg/mL-1000 μg/mL and the sensitivity is 62.5 μg/mL .

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