Target Name |
interleukin 5 (colony-stimulating factor, eosinophil) |
Alternative Names |
IL5Interleukin-5 ELISA Kit; IL-5 ELISA Kit; Eosinophil differentiation factor ELISA Kit; T-cell replacing factor ELISA Kit; TRF ELISA Kit |
Abbreviation |
IL5 |
Uniprot No. |
O08987 |
Species |
Cavia porcellus (Guinea pig) |
Sample Types |
serum, plasma, tissue homogenates |
Detection Range |
31.25 pg/mL-2000 pg/mL |
Sensitivity |
7.81 pg/mL |
Assay Time |
1-5h |
Sample Volume |
50-100ul |
Detection Wavelength |
450 nm |
Research Area |
Immunology
|
Assay Principle |
quantitative |
Measurement |
Sandwich |
Precision |
Intra-assay Precision (Precision within an assay): CV%<8% | | | | Three samples of known concentration were tested twenty times on one plate to assess. | | Inter-assay Precision (Precision between assays): CV%<10% | | | | Three samples of known concentration were tested in twenty assays to assess. | | | | | | | | | |
|
Linearity |
To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IL-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | | Sample | Serum(n=4) | | 1:1 | Average % | 90 | | Range % | 87-96 | | 1:2 | Average % | 94 | | Range % | 89-99 | | 1:4 | Average % | 103 | | Range % | 98-108 | | 1:8 | Average % | 100 | | Range % | 91-105 | |
|
Recovery |
The recovery of guinea pig IL-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
Sample Type | Average % Recovery | Range | | Serum (n=5) | 95 | 92-97 | | EDTA plasma (n=4) | 92 | 84-98 | | | | | | | | | | | | | | | |
|
Typical Data |
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
pg/ml | OD1 | OD2 | Average | Corrected | | 2000 | 2.516 | 2.489 | 2.503 | 2.391 | | 1000 | 1.384 | 1.356 | 1.370 | 1.258 | | 500 | 0.733 | 0.698 | 0.716 | 0.604 | | 250 | 0.384 | 0.375 | 0.380 | 0.268 | | 125 | 0.253 | 0.234 | 0.244 | 0.132 | | 62.5 | 0.187 | 0.181 | 0.184 | 0.072 | | 31.25 | 0.142 | 0.133 | 0.138 | 0.026 | | 0 | 0.113 | 0.111 | 0.112 | | |
|
|
Troubleshooting
and FAQs |
ELISA kit FAQs |
Storage |
Store at 2-8°C. Please refer to protocol. |
Lead Time |
3-5 working days |