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Horse Transforming growth factor beta-1(TGFB1) ELISA kit

Horse Transforming growth factor beta-1(TGFB1) ELISA kit

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Code	CSB-EL023446HO
Size	96T,5×96T,10×96T
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Target Data

Product Details

Target Name

transforming growth factor, beta 1

Alternative Names

TGFB1 ELISA Kit; Transforming growth factor beta-1 proprotein [Cleaved into: Latency-associated peptide ELISA Kit; LAP); Transforming growth factor beta-1 ELISA Kit; TGF-beta-1)] ELISA Kit

Abbreviation

TGFB1

Uniprot No.

O19011

Species

Equus caballus (Horse)

Sample Types

serum, plasma

Detection Range

3.12 pg/mL-200 pg/mL

Sensitivity

1.56 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Competitive

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of horse TGFB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	96
	Range %	92-100
1:200	Average %	103
	Range %	99-108
1:400	Average %	90
	Range %	86-94
1:800	Average %	92
	Range %	88-96

Recovery

The recovery of horse TGFB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	82-90
EDTA plasma (n=4)	94	90-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average
200	0.126	0.131	0.129
100	0.228	0.213	0.221
50	0.384	0.396	0.390
25	0.697	0.659	0.678
12.5	0.908	0.872	0.890
6.25	1.233	1.284	1.259
3.12	1.464	1.507	1.486
0	2.099	2.215	2.157

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

7-14 working days

Citations

Retinoic acid-mediated anti-inflammatory responses in equine immune cells stimulated by LPS and allogeneic mesenchymal stem cells Abdelhamid L.et al,Res Vet Sci,2017

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Target Data

Function	Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts (By similarity). Stimulates sustained production of collagen through the activation of CREB3L1 by regulated intramembrane proteolysis (RIP) (By similarity). Can promote either T-helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells. Mediates SMAD2/3 activation by inducing its phosphorylation and subsequent translocation to the nucleus. Can induce epithelial-to-mesenchymal transition (EMT) and cell migration in various cell types (By similarity).
Gene References into Functions	Within the limitations of the study design, production of COMP during healing of skin wounds does not appear to be influenced by wound type or anatomic site, nor does it appear to be correlated with TGF-beta1 concentrations. PMID: 21062324 Peritoneal TGF-beta(1) concentration was higher in horses with severe gastrointestinal diseases, in horses with an altered peritoneal fluid, and in nonsurvivors. PMID: 20636783
Subcellular Location	Secreted, extracellular space, extracellular matrix
Protein Families	TGF-beta family
Database Links	KEGG: ecb:100033900 STRING: 9796.ENSECAP00000009480 UniGene: Eca.6371

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Related pathways

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