Horse platelet-derived growth factor-BB(PDGF-BB) ELISA kit

Code CSB-EQ027241HO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details


This Horse PDGF-BB ELISA Kit was designed for the quantitative measurement of Horse PDGF-BB protein in serum, plasma,tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL.

Target Name platelet-derived growth factor-BB(PDGF-BB)
Abbreviation PDGF-BB
Species Equus caballus (Horse)
Sample Types serum, plasma,tissue homogenates
Detection Range 1.56 ng/mL-100 ng/mL
Sensitivity 0.39 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of horse PDGF-BB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 87  
Range % 82-91  
1:2 Average % 94  
Range % 91-99  
1:4 Average % 84  
Range % 81-87  
1:8 Average % 83  
Range % 80-86  
The recovery of horse PDGF-BB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 104 101-109  
EDTA plasma (n=4) 94 91-99  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml. OD1 OD2 Average Corrected  
100 2.132 2.106 2.119 1.997  
50 1.711 1.761 1.736 1.614  
25 1.214 1.245 1.230 1.108  
12.5 0.758 0.739 0.749 0.627  
6.25 0.513 0.534 0.524 0.402  
3.12 0.312 0.304 0.308 0.186  
1.56 0.181 0.183 0.182 0.060  
0 0.121 0.123 0.122    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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