| Description |
This Horse PDGF-BB ELISA Kit was designed for the quantitative measurement of Horse PDGF-BB protein in serum, plasma,tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL. |
| Target Name |
platelet-derived growth factor-BB(PDGF-BB) |
| Abbreviation |
PDGF-BB |
| Species |
Equus caballus (Horse) |
| Sample Types |
serum, plasma,tissue homogenates |
| Detection Range |
1.56 ng/mL-100 ng/mL |
| Sensitivity |
0.39 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular
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| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of horse PDGF-BB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
87 |
|
| Range % |
82-91 |
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| 1:2 |
Average % |
94 |
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| Range % |
91-99 |
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| 1:4 |
Average % |
84 |
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| Range % |
81-87 |
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| 1:8 |
Average % |
83 |
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| Range % |
80-86 |
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| Recovery |
| The recovery of horse PDGF-BB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
104 |
101-109 |
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| EDTA plasma (n=4) |
94 |
91-99 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml. |
OD1 |
OD2 |
Average |
Corrected |
|
| 100 |
2.132 |
2.106 |
2.119 |
1.997 |
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| 50 |
1.711 |
1.761 |
1.736 |
1.614 |
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| 25 |
1.214 |
1.245 |
1.230 |
1.108 |
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| 12.5 |
0.758 |
0.739 |
0.749 |
0.627 |
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| 6.25 |
0.513 |
0.534 |
0.524 |
0.402 |
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| 3.12 |
0.312 |
0.304 |
0.308 |
0.186 |
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| 1.56 |
0.181 |
0.183 |
0.182 |
0.060 |
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| 0 |
0.121 |
0.123 |
0.122 |
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| ELISA Data Analysis |
Watch ELISA data processing video & download Curve Expert if needed |
Troubleshooting
and FAQs |
ELISA kit FAQs |
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
