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Code | CSB-E08925m |
Size | 96T,5×96T,10×96T |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
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Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of mouse PDGF-BB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 85 | ||||
Range % | 82-88 | |||||
1:2 | Average % | 94 | ||||
Range % | 89-98 | |||||
1:4 | Average % | 104 | ||||
Range % | 97-109 | |||||
1:8 | Average % | 99 | ||||
Range % | 93-105 |
The recovery of mouse PDGF-BB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 95 | 89-101 | ||||
EDTA plasma (n=4) | 89 | 82-96 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This Mouse PDGF-BB ELISA Kit was designed for the quantitative measurement of Mouse PDGF-BB protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.18 ng/mL-12 ng/mL and the sensitivity is 0.047 ng/mL.
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