| Description |
This Mouse PDGF-BB ELISA Kit was designed for the quantitative measurement of Mouse PDGF-BB protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.18 ng/mL-12 ng/mL and the sensitivity is 0.047 ng/mL. |
| Target Name |
platelet-derived growth factor-BB,PDGF-BB |
| Abbreviation |
PDGF-BB |
| Species |
Mus musculus (Mouse) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.18 ng/mL-12 ng/mL |
| Sensitivity |
0.047 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular
|
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | | Three samples of known concentration were tested twenty times on one plate to assess. | | | Inter-assay Precision (Precision between assays): CV%<10% | | | | | Three samples of known concentration were tested in twenty assays to assess. | | | | | | | | | | |
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse PDGF-BB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | | Sample | Serum(n=4) | | | 1:1 | Average % | 85 | | | Range % | 82-88 | | | 1:2 | Average % | 94 | | | Range % | 89-98 | | | 1:4 | Average % | 104 | | | Range % | 97-109 | | | 1:8 | Average % | 99 | | | Range % | 93-105 | |
|
| Recovery |
| The recovery of mouse PDGF-BB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
| Sample Type | Average % Recovery | Range | | | Serum (n=5) | 95 | 89-101 | | | EDTA plasma (n=4) | 89 | 82-96 | | | | | | | | | | | | | | | | | |
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
 | ng/ml | OD1 | OD2 | Average | Corrected | | | 12 | 2.389 | 2.501 | 2.445 | 2.281 | | | 6 | 1.445 | 1.529 | 1.487 | 1.323 | | | 3 | 0.905 | 0.984 | 0.945 | 0.781 | | | 1.5 | 0.656 | 0.633 | 0.645 | 0.481 | | | 0.75 | 0.345 | 0.349 | 0.347 | 0.183 | | | 0.37 | 0.287 | 0.265 | 0.276 | 0.112 | | | 0.18 | 0.223 | 0.218 | 0.221 | 0.057 | | | 0 | 0.159 | 0.169 | 0.164 | | |
|
|
| ELISA Data Analysis |
Watch ELISA data processing video & download Curve Expert if needed |
Troubleshooting
and FAQs |
ELISA kit FAQs |
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
