Mouse platelet-derived growth factor-BB,PDGF-BB ELISA Kit

Code CSB-E08925m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Mouse PDGF-BB ELISA Kit was designed for the quantitative measurement of Mouse PDGF-BB protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.18 ng/mL-12 ng/mL and the sensitivity is 0.047 ng/mL.

Target Name platelet-derived growth factor-BB,PDGF-BB
Abbreviation PDGF-BB
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.18 ng/mL-12 ng/mL
Sensitivity 0.047 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse PDGF-BB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %85
Range %82-88
1:2Average %94
Range %89-98
1:4Average %104
Range %97-109
1:8Average %99
Range %93-105
Recovery
The recovery of mouse PDGF-BB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-101
EDTA plasma (n=4)8982-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
122.389 2.501 2.445 2.281
61.445 1.529 1.487 1.323
30.905 0.984 0.945 0.781
1.50.656 0.633 0.645 0.481
0.750.345 0.349 0.347 0.183
0.370.287 0.265 0.276 0.112
0.180.223 0.218 0.221 0.057
00.159 0.169 0.164
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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