Human Platelet-Derived Growth Factor-BB,PDGF-BB ELISA kit

Code CSB-E08923h
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Promotion
20% off above 2 kits (96T) per item
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase in six months till depleted. Apply now

Product Details

Abbreviation
PDGF-BB
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
31.25 pg/mL-2000 pg/mL
Sensitivity
7.81 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess. 

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human PDGF-BB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Recovery

The recovery of human PDGF-BB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

The human PDGF-BB ELISA Kit is engineered for accurate measurement of human PDGF-BB levels from samples including serum, plasma, or tissue homogenates. It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the PDGF-BB content in the sample. The color intensity is positively correlated with PDGF-BB content in the sample. The PDGF-BB concentration can be calculated according to the standard curve. This kit is tested with high sensitivity, strong specificity, good linearity, high precision and recovery, as well as lot-to-lot consistency.

PDGF-BB is a powerful promoter of cell proliferation and increases the expression of stem cell markers. It suppresses collagen production and mineralization but accelerates the maturation of collagen chains through increased LOX activity and SPARC expression. PDGF-BB binds to the PDGFR-β and subsequently activates several intracellular signaling cascades, including MAPK and PI3K/Akt pathways, which in turn activate the downstream targets mTOR and p70S6K. PDGF-BB-PDGFR-β signaling in brain pericytes is critical to the development, maintenance, and function of a healthy blood-brain barrier (BBB). It is also involved in the tissue repair and tumor progression effects and is released in response to pathogen-induced changes in the microenvironment acting as a rapid and early effector cytokine.

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