Human Platelet-Derived Growth Factor-BB,PDGF-BB ELISA kit

Platelet-derived growth factor-BB (PDGF-BB) is a potent mitogenic factor that plays key roles in cellular proliferation, migration, and angiogenesis. As a member of the PDGF family, PDGF-BB functions through binding to PDGF receptors and activating downstream signaling cascades that regulate wound healing, tissue repair, and vascular development. This growth factor is particularly important in mesenchymal cell biology. Researchers have studied its involvement in various pathological conditions including atherosclerosis, fibrosis, and cancer progression.

The Human Platelet-Derived Growth Factor-BB ELISA kit (CSB-E08923h) uses a quantitative sandwich measurement principle for detecting PDGF-BB in serum, plasma, and tissue homogenates. This assay offers a detection range of 31.25 pg/mL to 2000 pg/mL with a sensitivity of 7.81 pg/mL. The protocol requires 50-100 μL sample volume and can be completed within 1-5 hours. Detection is performed at 450 nm wavelength.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

This ELISA kit has been used in research examining platelet activation biomarkers and growth factor expression in various biological contexts. Studies have applied the kit to measure PDGF-BB levels in plasma samples and to evaluate growth factor secretion patterns from human cell cultures.

• Platelet activation studies: Measurement of PDGF-BB as a biomarker of platelet activation in plasma samples
• Renal tissue research: Quantification of PDGF levels in kidney tissue alongside other signaling molecules and growth factors
• Cell culture analysis: Detection of PDGF-BB secretion from human endothelial cell cultures as part of neurotrophic factor profiling studies