| Description |
This Rat PDGF-BB ELISA Kit was designed for the quantitative measurement of Rat PDGF-BB protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 39.063 pg/mL-2500 pg/mL and the sensitivity is 9.77 pg/mL. |
| Target Name |
platelet-derived growth factor-BB,PDGF-BB |
| Abbreviation |
PDGF-BB |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range |
39.063 pg/mL-2500 pg/mL |
| Sensitivity |
9.77 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular
|
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | | Three samples of known concentration were tested twenty times on one plate to assess. | | | Inter-assay Precision (Precision between assays): CV%<10% | | | | | Three samples of known concentration were tested in twenty assays to assess. | | | | | | | | | | |
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat PDGF-BB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | | Sample | Serum(n=4) | | | 1:1 | Average % | 104 | | | Range % | 101-107 | | | 1:2 | Average % | 97 | | | Range % | 94-100 | | | 1:4 | Average % | 88 | | | Range % | 83-90 | | | 1:8 | Average % | 89 | | | Range % | 84-93 | |
|
| Recovery |
| The recovery of rat PDGF-BB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
| Sample Type | Average % Recovery | Range | | | Serum (n=5) | 105 | 101-109 | | | EDTA plasma (n=4) | 110 | 107-113 | | | | | | | | | | | | | | | | | |
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
 | pg/ml | OD1 | OD2 | Average | Corrected | | | 2500 | 2.794 | 2.753 | 2.774 | 2.578 | | | 1250 | 2.204 | 2.200 | 2.202 | 2.006 | | | 625 | 1.530 | 1.501 | 1.516 | 1.320 | | | 312.5 | 0.901 | 0.925 | 0.913 | 0.717 | | | 156.25 | 0.635 | 0.652 | 0.644 | 0.448 | | | 78.125 | 0.459 | 0.435 | 0.447 | 0.251 | | | 39.063 | 0.376 | 0.368 | 0.372 | 0.176 | | | 0 | 0.197 | 0.194 | 0.196 | | |
|
|
| ELISA Data Analysis |
Watch ELISA data processing video & download Curve Expert if needed |
Troubleshooting
and FAQs |
ELISA kit FAQs |
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
