Rat platelet-derived growth factor-BB,PDGF-BB ELISA Kit

Code CSB-E08924r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details


This Rat PDGF-BB ELISA Kit was designed for the quantitative measurement of Rat PDGF-BB protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 39.063 pg/mL-2500 pg/mL and the sensitivity is 9.77 pg/mL.

Target Name platelet-derived growth factor-BB,PDGF-BB
Abbreviation PDGF-BB
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 39.063 pg/mL-2500 pg/mL
Sensitivity 9.77 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of rat PDGF-BB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %104
Range %101-107
1:2Average %97
Range %94-100
1:4Average %88
Range %83-90
1:8Average %89
Range %84-93
The recovery of rat PDGF-BB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 105101-109
EDTA plasma (n=4)110107-113
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
25002.794 2.753 2.774 2.578
12502.204 2.200 2.202 2.006
6251.530 1.501 1.516 1.320
312.50.901 0.925 0.913 0.717
156.250.635 0.652 0.644 0.448
78.1250.459 0.435 0.447 0.251
39.0630.376 0.368 0.372 0.176
00.197 0.194 0.196
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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