Name: Human 14-3-3 protein eta(YWHAH) ELISA kit
Brand: CUSABIO
SKU: CSB-EL026289HU

Product Details

Target Name

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta polypeptide

Alternative Names

14 3 3 protein eta ELISA Kit; 14-3-3 eta ELISA Kit; 14-3-3 protein eta ELISA Kit; 1433F_HUMAN ELISA Kit; Brain protein 14-3-3; eta isoform ELISA Kit; HGNC:12853 ELISA Kit; Protein AS1 ELISA Kit; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein 1 ELISA Kit; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein eta polypeptide ELISA Kit; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein; eta isoform ELISA Kit; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein; eta ELISA Kit; Tyrosine 3/tryptophan 5 monooxygenase activation protein eta polypeptide ELISA Kit; YWHA 1 ELISA Kit; YWHA1 ELISA Kit; Ywhah ELISA Kit

Abbreviation

YWHAH

Uniprot No.

Q04917

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.625 ng/mL-40 ng/mL

Sensitivity

0.156 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human YWHAH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	88-97
1:2	Average %	97
	Range %	93-103
1:4	Average %	85
	Range %	81-89
1:8	Average %	102
	Range %	86-105

Recovery

The recovery of human YWHAH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	86-98
EDTA plasma (n=4)	88	82-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.632	2.756	2.694	2.592
20	2.224	2.376	2.300	2.198
10	1.646	1.748	1.697	1.595
5	0.934	0.950	0.942	0.840
2.5	0.524	0.508	0.516	0.414
1.25	0.356	0.362	0.359	0.257
0.625	0.242	0.245	0.244	0.142
0	0.103	0.101	0.102

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human YWHAH ELISA Kit was designed for the quantitative measurement of Human YWHAH protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

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Customer Reviews and Q&A

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■ Q&A

I am interested in human 14-3-3eta ELISA kit
What kind of positive control was used to check the kit?
I am planning to use primary cell culture supernatant
I have an experience with other companies ELISA plate. That company used as positive control recombinant 14-3-3eta derived from YEAST. I tried as control recombinant 14-3-3eta derived from E.Coli at same concentration and got negative data. That is why I am searching for other ELISA kit and wondering if CUSABIO had checked ELISA kit with serum, cells lysate also, not only recombinant protein.
One more question. What is the binding site of the anti-14-3-3eta antibody used at this ELISA kit? Thank you!
Excuse me, this is the product I am interested in https://www.cusabio.com/ELISA-Kit/Human-14-3-3-protein-etaYWHAH-ELISA-kit-112977.html

Thanks for your inquir!Our elisa kits are provided with the standard in it.The standard performs as a quality control and it comes as a complete system together with other reagents in the kit.
Regarding this elisa kit,the standard is express with CHO and 2 antibodies are used in it,mouse monoclonal antibody and goat polyclonal antibody.While the specific recognition site is not clear.
We tested and validated this kit on serum and plasma samples and this kit is usually used to detect serum and plasma samples.For cells lysate sample,we suggest the customer need do pre-testing first.If the customer uses the recombinant protein of the same structure with the natural protein,then it is ok to use it to detect the recombinant protein.If the customer must detect the recombinant protein,he/she had better to use the the recombinant protein expressed by eukaryote.While for the specific situation,pls refer to the customer's actual testing results.Natural protein is for sure the best.
As to the elisa kits from other brands,we did not ever analyzed and it is hard to comment.For the mentioned negative results,we suppose it happens.Pls do the specific analysis accordingly.

Target Background

Function
(From Uniprot)

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.

Gene References into Functions

Results show that YWHAZ amplification indicates a better survival trend in bladder cancer. Its knockdown promotes both in vitro and in vivo tumorigenesis in bladder cancer and may be a novel biomarker for bladder cancer. PMID: 29512774
The impact of AKT1 on glucocorticoid receptor (GR)-induced transcriptional activity in cooperation with phospho-serine/threonine-binding protein 14-3-3, was examined. PMID: 27717743
14-3-3 eta isoform colocalizes TDP-43 on the coarse granules in the anterior horn cells of patients with sporadic amyotrophic lateral sclerosis. PMID: 27256400
Extracellular 14-3-3eta activates key signalling cascades and induces factors associated with the pathogenesis of rheumatoid arthritis PMID: 24751211
This study identified YWHAH significantly associated loci with a biologically plausible role in schizophrenia. PMID: 24507884
14-3-3eta may be required for mitotic progression and may be considered as a novel anti-cancer strategy in combination with microtubule inhibitors. PMID: 22562251
This is the first genetic association study of YWHAH with sporadic Creutzfeldt-Jakob disease populations. PMID: 21739144
relocation of p33ING1b from the nucleus to the cytoplasm, where the protein is tethered by 14-3-3eta, participates in tumorigenesis and progression in HNSCC PMID: 21432775
The 14-3-3 eta, beta, gamma and sigma isoforms were negatively expressed in meningioma PMID: 20388496
phosphorylation of 14-3-3 binding site of myeloid leukemia factor 1 by MADM PMID: 12176995
Data demonstrate that 14-3-3 proteins are colocalized with Lewy bodies in Parkinson disease, but there was no specific staining for the 14-3-3 eta subunit. PMID: 12480176
gremlin 1 is overexpressed in human cancers and interacts with YWHAH protein PMID: 16545136
The association of YWHAH with bipolar disorder in a large sample, consisting of 1211 subjects from 318 nuclear families including 554 affected offspring, was investigated. PMID: 19160447

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Protein Families

14-3-3 family

Tissue Specificity

Expressed mainly in the brain and present in other tissues albeit at lower levels.

Database Links

HGNC: 12853

OMIM: 113508

KEGG: hsa:7533

STRING: 9606.ENSP00000248975

UniGene: Hs.226755

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Code	CSB-EL026289HU
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.