YWHAH Antibody

Datasheet

Code	CSB-PA02174A0Rb
Size	US$166
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Image	Western blot All lanes: YWHAH antibody at 2µg/ml + Mouse spleen tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 29 kDa Observed band size: 29 kDa Western Blot Positive WB detected in: HepG2 whole cell lysate, Mouse kidney tissue, Mouse brain tissue, Mouse thymus tissue All lanes: YWHAH antibody at 4.5µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 29 kDa Observed band size: 29 kDa Immunohistochemistry of paraffin-embedded human prostate tissue using CSB-PA02174A0Rb at dilution of 1:20 IHC image of CSB-PA02174A0Rb diluted at 1:400 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA02174A0Rb diluted at 1:400 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-PA02174A0Rb at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) YWHAH Polyclonal antibody

Uniprot No.

Q04917

Target Names

YWHAH

Alternative Names

14 3 3 protein eta antibody; 14-3-3 eta antibody; 14-3-3 protein eta antibody; 1433F_HUMAN antibody; Brain protein 14-3-3; eta isoform antibody; HGNC:12853 antibody; Protein AS1 antibody; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein 1 antibody; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein eta polypeptide antibody; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein; eta isoform antibody; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein; eta antibody; Tyrosine 3/tryptophan 5 monooxygenase activation protein eta polypeptide antibody; YWHA 1 antibody; YWHA1 antibody; Ywhah antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human 14-3-3 protein eta protein (4-246AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The YWHAH Antibody (Product code: CSB-PA02174A0Rb) is Non-conjugated. For YWHAH Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA02174B0Rb	YWHAH Antibody, HRP conjugated	ELISA
FITC	CSB-PA02174C0Rb	YWHAH Antibody, FITC conjugated
Biotin	CSB-PA02174D0Rb	YWHAH Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:20-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.

Gene References into Functions

Results show that YWHAZ amplification indicates a better survival trend in bladder cancer. Its knockdown promotes both in vitro and in vivo tumorigenesis in bladder cancer and may be a novel biomarker for bladder cancer. PMID: 29512774
The impact of AKT1 on glucocorticoid receptor (GR)-induced transcriptional activity in cooperation with phospho-serine/threonine-binding protein 14-3-3, was examined. PMID: 27717743
14-3-3 eta isoform colocalizes TDP-43 on the coarse granules in the anterior horn cells of patients with sporadic amyotrophic lateral sclerosis. PMID: 27256400
Extracellular 14-3-3eta activates key signalling cascades and induces factors associated with the pathogenesis of rheumatoid arthritis PMID: 24751211
This study identified YWHAH significantly associated loci with a biologically plausible role in schizophrenia. PMID: 24507884
14-3-3eta may be required for mitotic progression and may be considered as a novel anti-cancer strategy in combination with microtubule inhibitors. PMID: 22562251
This is the first genetic association study of YWHAH with sporadic Creutzfeldt-Jakob disease populations. PMID: 21739144
relocation of p33ING1b from the nucleus to the cytoplasm, where the protein is tethered by 14-3-3eta, participates in tumorigenesis and progression in HNSCC PMID: 21432775
The 14-3-3 eta, beta, gamma and sigma isoforms were negatively expressed in meningioma PMID: 20388496
phosphorylation of 14-3-3 binding site of myeloid leukemia factor 1 by MADM PMID: 12176995
Data demonstrate that 14-3-3 proteins are colocalized with Lewy bodies in Parkinson disease, but there was no specific staining for the 14-3-3 eta subunit. PMID: 12480176
gremlin 1 is overexpressed in human cancers and interacts with YWHAH protein PMID: 16545136
The association of YWHAH with bipolar disorder in a large sample, consisting of 1211 subjects from 318 nuclear families including 554 affected offspring, was investigated. PMID: 19160447

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Protein Families

14-3-3 family

Tissue Specificity

Expressed mainly in the brain and present in other tissues albeit at lower levels.

Database Links

HGNC: 12853

OMIM: 113508

KEGG: hsa:7533

STRING: 9606.ENSP00000248975

UniGene: Hs.226755

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Related pathways

Hippo signaling pathway PI3K-Akt signaling pathway Cell cycle

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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