Human Acyloxyacyl hydrolase(AOAH) ELISA kit

Instructions
Code CSB-EL001853HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Target Name acyloxyacyl hydrolase (neutrophil)
Alternative Names Acyloxyacyl hydrolase (neutrophil) ELISA Kit; Acyloxyacyl hydrolase large subunit ELISA Kit; AOAH ELISA Kit; AOAH_HUMAN ELISA Kit
Abbreviation AOAH
Uniprot No. P28039
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human AOAH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 88
Range % 81-96
1:2 Average % 97
Range % 91-103
1:4 Average % 99
Range % 82-106
1:8 Average % 87
Range % 81-92
Recovery
The recovery of human AOAH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 97 93-102
EDTA plasma (n=4) 101 94-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
10 2.680 2.769 2.725 2.620
5 2.246 2.124 2.185 2.080
2.5 1.495 1.387 1.441 1.336
1.25 0.820 0.837 0.829 0.724
0.625 0.475 0.486 0.481 0.376
0.312 0.281 0.272 0.277 0.172
0.156 0.204 0.210 0.207 0.102
0 0.104 0.105 0.105  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Target Data

Function Removes the secondary (acyloxyacyl-linked) fatty acyl chains from the lipid A region of bacterial lipopolysaccharides
Gene References into Functions
  1. analysis of AOAH function through its crystal structure and complex with lipopolysaccharide PMID: 29343645
  2. AOAH - Gene involved in innate immunity that have been associated with Chronic Rhinosinusitis. PMID: 27888910
  3. AOAH rs60023210 variants may be associated with carotid intima-media thickness at bifurcation. PMID: 26319989
  4. Polymorphisms in RYBP and AOAH genes are associated with chronic rhinosinusitis in a Chinese population. PMID: 22723975
Subcellular Location Secreted, Cytoplasmic vesicle
Database Links

HGNC: 548

OMIM: 102593

KEGG: hsa:313

STRING: 9606.ENSP00000258749

UniGene: Hs.488007

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2020 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1