Human Acyloxyacyl hydrolase(AOAH) ELISA kit

Code CSB-EL001853HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
acyloxyacyl hydrolase (neutrophil)
Alternative Names
Acyloxyacyl hydrolase (neutrophil) ELISA Kit; Acyloxyacyl hydrolase large subunit ELISA Kit; AOAH ELISA Kit; AOAH_HUMAN ELISA Kit
Abbreviation
AOAH
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
0.156 ng/mL-10 ng/mL
Sensitivity
0.039 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human AOAH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 88
Range % 81-96
1:2 Average % 97
Range % 91-103
1:4 Average % 99
Range % 82-106
1:8 Average % 87
Range % 81-92
Recovery
The recovery of human AOAH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 97 93-102
EDTA plasma (n=4) 101 94-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
10 2.680 2.769 2.725 2.620
5 2.246 2.124 2.185 2.080
2.5 1.495 1.387 1.441 1.336
1.25 0.820 0.837 0.829 0.724
0.625 0.475 0.486 0.481 0.376
0.312 0.281 0.272 0.277 0.172
0.156 0.204 0.210 0.207 0.102
0 0.104 0.105 0.105  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human AOAH ELISA Kit was designed for the quantitative measurement of Human AOAH protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

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Target Background

Function
(From Uniprot)
Removes the secondary (acyloxyacyl-linked) fatty acyl chains from the lipid A region of bacterial lipopolysaccharides. By breaking down LPS, terminates the host response to bacterial infection and prevents prolonged and damaging inflammatory responses. In peritoneal macrophages, seems to be important for recovery from a state of immune tolerance following infection by Gram-negative bacteria.
Gene References into Functions
  1. analysis of AOAH function through its crystal structure and complex with lipopolysaccharide PMID: 29343645
  2. AOAH - Gene involved in innate immunity that have been associated with Chronic Rhinosinusitis. PMID: 27888910
  3. AOAH rs60023210 variants may be associated with carotid intima-media thickness at bifurcation. PMID: 26319989
  4. Polymorphisms in RYBP and AOAH genes are associated with chronic rhinosinusitis in a Chinese population. PMID: 22723975
Subcellular Location
Secreted. Cytoplasmic vesicle.
Database Links

HGNC: 548

OMIM: 102593

KEGG: hsa:313

STRING: 9606.ENSP00000258749

UniGene: Hs.488007

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