Name: Recombinant Human Acyloxyacyl hydrolase (AOAH)
Brand: CUSABIO
SKU: CSB-EP001853HU

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

AOAH

Uniprot No.

P28039

Research Area

Immunology

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

35-575aa

Target Protein Sequence

LSNGHTCVGCVLVVSVIEQLAQVHNSTVQASMERLCSYLPEKLFLKTTCYLVIDKFGSDIIKLLSADMNADVVCHTLEFCKQNTGQPLCHLYPLPKETWKFTLQKARQIVKKSPILKYSRSGSDICSLPVLAKICQKIKLAMEQSVPFKDVDSDKYSVFPTLRGYHWRGRDCNDSDESVYPGRRPNNWDVHQDSNCNGIWGVDPKDGVPYEKKFCEGSQPRGIILLGDSAGAHFHISPEWITASQMSLNSFINLPTALTNELDWPQLSGATGFLDSTVGIKEKSIYLRLWKRNHCNHRDYQNISRNGASSRNLKKFIESLSRNKVLDYPAIVIYAMIGNDVCSGKSDPVPAMTTPEKLYSNVMQTLKHLNSHLPNGSHVILYGLPDGTFLWDNLHNRYHPLGQLNKDMTYAQLYSFLNCLQVSPCHGWMSSNKTLRTLTSERAEQLSNTLKKIAASEKFTNFNLFYMDFAFHEIIQEWQKRGGQPWQLIEPVDGFHPNEVALLLLADHFWKKVQLQWPQILGKENPFNPQIKQVFGDQGGH
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

62.4 kDa

Protein Length

Full Length of Mature Protein

Tag Info

C-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Customer Reviews and Q&A

■ Customer Reviews

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■ Q&A

I'm wondering what the maximum concentration and lowest glycerol percentages you’ve achieved previously if you have at all?
They want to know if increasing the concentration and decreasing the glycerol in the storage buffer will influence the protein activity and stability?

For CSB-EP001853HU, previously, we sent lyophilized powder for most of the time, and the concentration before freeze-drying was 1.35mg/ml.
If deliver with liquid form, we can increase the concentration by 0.46mg/ml, and then send it after reconstitution.
Glycerol has a protective effect on proteins. Firstly, it can increase the stability of the protein. Secondly, it can lower the freezing point of the system and facilitate the preservation of protein at low temperatures. Especially when the protein is repeatedly frozen and thawed, protein damage (precipitated or inactivated) can be avoided as much as possible. As for the final concentration of glycerol, in principle, the final concentration ranges from 5% to 50% is OK, which may be related to the storage time. The protein may be unstable without glycerol.
When the protein is stable, the activity may not be affected. Regarding the activity of CSB-EP001853HU, I'm sorry that we haven't tested it for the time being and haven't received feedback from customers, so we can't guarantee 100% activity.

Can you increase the concentration of the human recombinant AOAH? The one we previously bought has a concentration of 0.46 mg/mL;
Can you lower the percentage of glycerol in the storage buffer? Currently, the buffer contains 50% glycerol.

Regarding CSB-EP001853HU, we can increase the concentration of the human recombinant AOAH according to your demand, and can lower the percentage of glycerol in the storage buffer.
Please remark these requirements when placing the order.

What are the maximum concentration and lowest glycerol percentages you’ve achieved previously if you have at all?
I would like to know if increasing the concentration and decreasing the glycerol in the storage buffer will influence the protein activity and stability?

For CSB-EP001853HU, previously, we sent lyophilized powder for most of the time, and the concentration before freeze-drying was 1.35mg/ml.
If deliver with liquid form, we can increase the concentration by 0.46mg/ml, and then send it after reconstitution.

Glycerol has a protective effect on proteins. Firstly, it can increase the stability of the protein. Secondly, it can lower the freezing point of the system and facilitate the preservation of protein at low temperatures. Especially when the protein is repeatedly frozen and thawed, protein damage (precipitated or inactivated) can be avoided as much as possible. As for the final concentration of glycerol, in principle, the final concentration ranges from 5% to 50% is OK, which may be related to the storage time. The protein may be unstable without glycerol.

When the protein is stable, the activity may not be affected. Regarding the activity of CSB-EP001853HU, I'm sorry that we haven't tested it for the time being and haven't received feedback from customers, so we can't guarantee 100% activity.

Target Background

Function

Removes the secondary (acyloxyacyl-linked) fatty acyl chains from the lipid A region of bacterial lipopolysaccharides. By breaking down LPS, terminates the host response to bacterial infection and prevents prolonged and damaging inflammatory responses. In peritoneal macrophages, seems to be important for recovery from a state of immune tolerance following infection by Gram-negative bacteria.

Gene References into Functions

analysis of AOAH function through its crystal structure and complex with lipopolysaccharide PMID: 29343645
AOAH - Gene involved in innate immunity that have been associated with Chronic Rhinosinusitis. PMID: 27888910
AOAH rs60023210 variants may be associated with carotid intima-media thickness at bifurcation. PMID: 26319989
Polymorphisms in RYBP and AOAH genes are associated with chronic rhinosinusitis in a Chinese population. PMID: 22723975

Subcellular Location

Secreted. Cytoplasmic vesicle.

Database Links

HGNC: 548

OMIM: 102593

KEGG: hsa:313

STRING: 9606.ENSP00000258749

UniGene: Hs.488007

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Code	CSB-EP001853HU
Abbreviation	Recombinant Human AOAH protein
MSDS	MSDS Datasheet
Size	$306
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Acyloxyacyl hydrolase (AOAH)

Product Details

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Customer Reviews and Q&A

Target Background

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