Human B-cell lymphoma-extra large (Bcl-xl)ELISA kit

Code CSB-EL027199HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details


This Human Bcl-xl ELISA Kit was designed for the quantitative measurement of Human Bcl-xl protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

Target Name B-cell lymphoma-extra large (Bcl-xl)ELISA kit
Alternative Names
AI325008 ELISA Kit; BAD ELISA Kit; BAD_HUMAN ELISA Kit; BBC 2 ELISA Kit; BBC2 ELISA Kit; BBC6 ELISA Kit; Bcl 2 Antagonist of Cell Death ELISA Kit; Bcl 2 Binding Component 6 ELISA Kit; BCL X / BCL 2 Binding Protein ELISA Kit; BCL X Binding Protein ELISA Kit; Bcl XL/Bcl 2 Associated Death Promoter ELISA Kit; Bcl-2-binding component 6 ELISA Kit; Bcl-2-like protein 8 ELISA Kit; Bcl-XL/Bcl-2-associated death promoter ELISA Kit; Bcl2 antagonist of cell death ELISA Kit; BCL2 antagonist of cell death protein ELISA Kit; BCL2 associated agonist of cell death ELISA Kit; Bcl2 Associated Death Promoter ELISA Kit; BCL2 binding component 6 ELISA Kit; BCL2 binding protein ELISA Kit; Bcl2 Like 8 Protein ELISA Kit; Bcl2-L-8 ELISA Kit; BCL2L8 ELISA Kit; Proapoptotic BH3 Only Protein ELISA Kit
Abbreviation Bcl-xl
Uniprot No. Q92934
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of human Bcl-xl in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %84
Range %80-89
1:2Average %97
Range %92-101
1:4Average %105
Range %100-110
1:8Average %93
Range %86-98
The recovery of human Bcl-xl spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-99
EDTA plasma (n=4)9085-94
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
102.415 2.352 2.384 2.190
51.934 1.876 1.905 1.711
2.51.405 1.504 1.455 1.261
1.250.902 0.939 0.921 0.727
0.6250.544 0.524 0.534 0.340
0.3120.378 0.359 0.369 0.175
0.1560.291 0.309 0.300 0.106
00.191 0.197 0.194
ELISA Data Analysis ELISA Standard Curve & Curve Expert software
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

(From Uniprot)
Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
Gene References into Functions
  1. High BAD expression is associated with cisplatinresistant oral cancer. PMID: 29956797
  2. Bcl-2 agonist of cell death (BAD) has pro-apoptosis and pro-survival functions involved in cancer development [Review]. PMID: 29175460
  3. The findings suggest that experimental hyperthermia (EH) exposure leads to simultaneous activation of molecular switches of apoptosis (BCL2 and BAD) in cells of the follicular epithelium of the ovaries on days 3 and 4 after EH. PMID: 29658076
  4. The positive correlation of Bad expression with nodule size and a relative decrease in the mRNA expression level of Bad in benign thyroid nodules suggest that Bad may be an important regulator of thyroid cell apoptosis. PMID: 29695560
  5. Data suggest that ECAD, STAT3, Bak, and Bcl-xL are expressed in affected endometrial tissues of women with endometrioid adenocarcinoma depending on neoplasm staging and cell differentiation. This study was conducted using immunohistochemistry of surgically resected tissues. (ECAD = E-cadherin; STAT3 = signal transducer and activator of transcription 3 protein; Bak = pro-apoptotic protein BAK) PMID: 28937296
  6. cyclin D1 was downregulated, whereas Bcell lymphoma 2associated agonist of cell death (BAD) was upregulated following RAC1 knockdown in colon cancer cells. PMID: 29286138
  7. we have found that a subgroup of colorectal cancers, defined by having either KRAS or BRAF (KRAS/BRAF) mutations and BCL2L1 (encoding BCL-XL) amplification, can be effectively targeted by simultaneous inhibition of BCL-XL (with ABT-263) and MCL1 (with YM-155). PMID: 28611106
  8. BAD phosphorylation is essential in the cytoprotective effect of vasoactive intestinal peptide on cancer stem cells. PMID: 28569785
  9. NDRG2 could inhibit Bad degradation by increasing its protein stability in breast cancer cells. PMID: 28423695
  10. Taken together, our results provide a structural basis for the binding mechanism between DJ-1 and Bcl-XL, which will contribute to molecular understanding of the role of mitochondrial DJ-1 in Bcl-XL regulation in response to oxidative stress. PMID: 29175327
  11. We will then review how the apoptotic and autophagic functions of Bcl-xL are modified by this post-translational modifications, and how this impacts on its oncogenic properties. PMID: 28645514
  12. the membrane localization of BCL-xL enforces its control over cell survival and, importantly, limits the pro-apoptotic effects of BH3 mimetics by selectively influencing BCL-xL binding to key pro-apoptotic effectors. PMID: 28009301
  13. The long unstructured region of Bcl-xl modulates its structural dynamics. PMID: 28486788
  14. Short-term treatment of nascent melanoma tumors with PAK inhibitors that block RhoJ signaling halts the growth of BRAF mutant melanoma tumors in vivo and induces apoptosis in melanoma cells in vitro via a BAD-dependent mechanism. As up to 50% of BRAF mutant human melanomas express high levels of RhoJ, these studies nominate the RhoJ-BAD signaling network as a therapeutic vulnerability for fledgling BRAF mutant human tumor PMID: 28753606
  15. Recent studies that combine experiments in yeast and in mammalian cells have shown the unexpected effect of the anti-apoptotic protein Bcl-xL on the priming of Bax. As demonstrated with the BH3-mimetic molecule ABT-737, this property of Bcl-xL, and of Bcl-2, is crucial to elaborate about how apoptosis could be reactivated in tumoral cells. PMID: 27112371
  16. the accumulation of reactive oxygen species (ROS) in cells expressing JAK2V617F compromises the NHE-1/Bcl-xL deamidation pathway by repressing NHE-1 upregulation in response to DNA damage. hematopoietic stem cells (HSCs), FOXO3A is largely localized within the nuclei despite the presence of JAK2V617F mutation, suggesting that JAK2-FOXO signaling has a different effect on progenitors compared with stem cells. PMID: 26234675
  17. These results identify beta3 integrin signaling via repression of BAD as an important survival pathway used by breast cancer cells to evade chemotherapy induced stress. PMID: 27235542
  18. BAD mutation is associated with maturity-onset diabetes of the young. PMID: 27935851
  19. miR-377 was markedly downregulated in HCC cell lines and primary human HCC tissues. The decreased expression of miR-377 contributes to the upregulation of Bcl-xL expression by targeting its 3'-untranslated region (3'-UTR). PMID: 28081730
  20. By the pharmacologic targeting of BCL2, MCL1, and BCL-XL, we demonstrated that diffuse large B-cell lymphoma can be divided into BCL2-dependent and MCL1-dependent subgroups with a less pronounced role left for BCL-XL. PMID: 26467384
  21. increased platelet apoptosis and activation as well as reduced expression of Bcl-xL, increased expression of Bax and caspase-3 activity were found in platelets after treated with ITP plasma in comparison with control plasma. PMID: 26712345
  22. These findings demonstrated that Akt is related to NF-kappaB and Bad signaling pathway possibly playing a direct role in the progression of liver cancer. Thus, Akt might be an important and potential treatment choice for the clinical diagnosis and treatment in the future. PMID: 26892230
  23. Bh3 domain induced conformational changes in Bcl-Xl revealed by crystal structure and comparative analysis. PMID: 25907960
  24. we can conclude that patients with small cell lung carcinoma represent downregulation of Bad and the latter could be served as a useful biomarker for the outcomes of SCLC. PMID: 26722503
  25. Bcl-xL is responsible for TRAIL resistance in human pancreatic cancer cells. Bcl-2 family inhibitors could represent promising reagents to sensitize human pancreatic cancers to TRAIL. PMID: 26506422
  26. This studypredict response ketogenic dietary therapies. showed that Common variants in KCNJ11 and BAD do not responase to ketogenic diet therapy. PMID: 26590798
  27. Bcl-xL binds to dual BH3-like domains in the InsP3 receptor carboxyl terminus and regulates control of cell viability. PMID: 26976600
  28. LA provoked a down regulation of two anti-apoptotic proteins, Mcl-1 and Bcl-xL protein and a strong induction of the BH3-only protein Bim. PMID: 26063499
  29. Valproic acid sensitized TRAIL-resistant papillary thyroid carcinoma cells to apoptotic cell death through involvement of Nrf2 and Bcl-xL. PMID: 26721202
  30. A Novel Naphthalimide Compound Restores p53 Function in Non-small Cell Lung Cancer by Reorganizing the Bak.Bcl-xl Complex and Triggering Transcriptional Regulation. PMID: 26668309
  31. These data suggest that miR-BART20-5p plays an important role in latency maintenance and tumor persistence of Epstein-Barr virus-associated gastric carcinoma by inhibiting BAD-mediated caspase-3-dependent apoptosis. PMID: 26581978
  32. Taken together, these data indicate that the downregulation of Bad and Bim plays a significant role in the autophagy-induced chemoresistance of hepatocellular carcinoma cells. PMID: 24947039
  33. These data suggest that Bcl-XL binds to RyR channels via its BH4 domain, but also its BH3 domain, more specific Lys87, contributes to the interaction. PMID: 25872771
  34. The BAD-mediated apoptotic pathway is thus associated with the development of human cancers likely influenced by the protein levels of pBAD. PMID: 25653146
  35. Study support that mitochondrial ERb prevents cell apoptosis through its interaction with bad protein and the mitochondrial apoptotic pathway in a ligand-independent manner. PMID: 25524600
  36. In resistant cells, RAS effector pathways maintained BAD phosphorylation in the presence of JAK inhibitors, yielding a specific dependence on BCL-XL for survival. PMID: 25538080
  37. BAD expression correlates with disease stage in prostate cancer, suggesting a role of BAD in tumor advancement. PMID: 25215949
  38. Results suggest that regulation of the proapoptotic activity of BAD plays a key role in the pathogenic mechanisms resulting in primary pigmented nodular adrenocortical disease tumor formation. PMID: 24865460
  39. BAD is down-regulated in breast cancer. PMID: 25499972
  40. Rapamycin-enhanced mitomycin C-induced apoptotic death is mediated through the S6K1-Bad-Bak pathway in peritoneal carcinomatosis. PMID: 24901052
  41. We observed higher expression levels of BCL-2, BCL-XL, BAX, and BAD genes in postmenopausal patients with pelvic organ prolapse compared with controls as well as overexpression of all four genes in parametrial tissue compared with vaginal tissue. PMID: 24614958
  42. Cur-NPs upregulated the protein expression levels of Bad and downregulated the protein expression level of p-Akt in U2OS cells PMID: 24247158
  43. Using gene reporter assays, we show that promoter variations in 11 intrinsic apoptosis genes, including ADPRT, APAF1, BCL2, BAD, BID, MCL1, BIRC4, BCL2L1, ENDOG, YWHAB, and YWHAQ, influence promoter activity in an allele-specific manner. PMID: 24038028
  44. BAD dephosphorylation and decreased expression of MCL1 induce rapid apoptosis in prostate cancer cells. PMID: 24040284
  45. Our results identify for the first time the downstream targets of insulin, cyclin D1, and BAD, elucidate a new molecular mechanism of insulin in promoting cell proliferation and apoptosis PMID: 23794242
  46. Platelet-derived growth factor-C (PDGF-C) induces anti-apoptotic effects on macrophages through Akt and Bad phosphorylation. PMID: 24421315
  47. AIF-1 can protect rheumatoid arthritis fibroblast-like synoviocytes from apoptosis induced by NO by upregulating the expression of p-Akt and p-BAD. PMID: 23547889
  48. study provided clinical evidence that loss of Bad is an independent and powerful predictor of adverse prognosis in non-small cell lung cancer PMID: 21918885
  49. These data indicate that influenza viruses carefully modulate the activation of the apoptotic pathway that is dependent on the regulatory function of BAD and that failure of apoptosis activation resulted in unproductive viral replication. PMID: 23135712
  50. RNAi-mediated silencing of STAT1 in soft tissue sarcoma (STS) cells was sufficient to increase expression of the apoptotic mediators Fas and Bad and to elevate the sensitivity of STS cells to Fas-mediated apoptosis PMID: 22805310

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Subcellular Location Mitochondrion outer membrane. Cytoplasm.
Protein Families Bcl-2 family
Tissue Specificity Expressed in a wide variety of tissues.
Database Links

HGNC: 936

OMIM: 603167

KEGG: hsa:572

STRING: 9606.ENSP00000309103

UniGene: Hs.370254

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