Product Details

Description

This Human BMP3 ELISA Kit was designed for the quantitative measurement of Human BMP3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

Target Name

bone morphogenetic protein 3

Alternative Names

BMP-3 ELISA Kit; BMP-3A ELISA Kit; bmp3 ELISA Kit; BMP3_HUMAN ELISA Kit; BMP3A ELISA Kit; Bone morphogenetic protein 3 (osteogenic) ELISA Kit; Bone morphogenetic protein 3 ELISA Kit; Bone morphogenetic protein 3A ELISA Kit; Osteogenin ELISA Kit

Abbreviation

BMP3

Uniprot No.

P12645

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

15.6 pg/mL-1000 pg/mL

Sensitivity

3.9 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human BMP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	82-91
1:2	Average %	102
	Range %	97-106
1:4	Average %	94
	Range %	87-98
1:8	Average %	93
	Range %	86-97

Recovery

The recovery of human BMP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	99-108
EDTA plasma (n=4)	92	87-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.512	2.633	2.573	2.399
500	1.834	1.948	1.891	1.717
250	1.176	1.218	1.197	1.023
125	0.756	0.774	0.765	0.591
62.5	0.463	0.473	0.468	0.294
31.2	0.289	0.296	0.293	0.119
15.6	0.225	0.235	0.230	0.056
0	0.173	0.175	0.174

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Related Products

Target Background

Function (From Uniprot)	Growth factor of the TGF-beta superfamily that plays an essential role in developmental process by inducing and patterning early skeletal formation and by negatively regulating bone density. Antagonizes the ability of certain osteogenic BMPs to induce osteoprogenitor differentitation and ossification. Initiates signaling cascades by associating with type II receptor ACVR2B to activate SMAD2-dependent and SMAD-independent signaling cascades including TAK1 and JNK pathways.
Gene References into Functions	This study demonstrated that there was a significantly higher frequency of BMP3 methylated DNA in plasma in patients with polyps versus healthy controls with a sensitivity and specificity of 40 and 94%, respectively. PMID: 29892846 BMP3 - the biomarker of a currently in-use multi-target stool DNA test was commonly expressed in tumor tissue specimens, independent of Fecal Immunochemical Test result. PMID: 28044229 Methylation level in stool decreases dramatically following colorectal cancer resection PMID: 24993691 Bone morphogenic protein 3 signaling in the regulation of osteogenesis. PMID: 23127436 As an addition to PRKG2 and RASGEFIB genes, we propose to include BMP3 gene as the principal determinant of the observed common phenotype. PMID: 22303795 a critical link between HNF1A-MODY-induced alterations in Bmp-3 expression and insulin gene levels PMID: 21628466 Features supportive of positive selection in the BMP3 gene were found including the presence of an excess of nonsynonymous mutations in modern humans, and a significantly lower genetic diversity that deviates from neutrality PMID: 20532035 Data suggest that promotor region methylation of the BMP3 gene may cause gastric carcinoma in Chinese people. PMID: 20238409 These results demonstrate that BMP-3 stimulates mesenchymal stem cell proliferation via the TGF-beta/activin signaling pathway. PMID: 20143330 The expression signals of BMP3 mRNA in malignant schwannoma were relatively lower than in benign lesions PMID: 11642720 BMP-3 receptor specificity is controlled by the interaction of Lys-30 of BMP-3 with Glu-76 of ActRIIb PMID: 17924656 BMP3 silencing is an early and frequent event in colorectal tumors progressing via the serrated and traditional pathways. PMID: 18311777 Show More Hide All
Subcellular Location	Secreted.
Protein Families	TGF-beta family
Tissue Specificity	Expressed in adult and fetal cartilage.
Database Links	HGNC: 1070 OMIM: 112263 KEGG: hsa:651 STRING: 9606.ENSP00000282701 UniGene: Hs.387411

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Check Code：

Code	CSB-EL002739HU
Size	96T,5×96T,10×96T
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Human Bone morphogenetic protein 3(BMP3) ELISA kit

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