Human Carcinoembryonic antigen-related cell adhesion molecule 8(CEACAM8) ELISA kit

Instructions
Code CSB-EL005168HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name carcinoembryonic antigen-related cell adhesion molecule 8
Alternative Names Carcinoembryonic antigen CGM6 ELISA Kit; Carcinoembryonic antigen gene family member 6 ELISA Kit; Carcinoembryonic antigen related cell adhesion molecule 8 ELISA Kit; Carcinoembryonic antigen-related cell adhesion molecule 8 ELISA Kit; CD 66b ELISA Kit; CD 67 ELISA Kit; CD66b ELISA Kit; CD66b antigen ELISA Kit; CD67 ELISA Kit; CD67 antigen ELISA Kit; CEACAM 8 ELISA Kit; CEACAM8 ELISA Kit; CEAM8_HUMAN ELISA Kit; CGM 6 ELISA Kit; CGM6 ELISA Kit; NCA 95 ELISA Kit; NCA95 ELISA Kit; Non-specific cross-reacting antigen NCA-95 ELISA Kit; Nonspecific cross reacting antigen NCA 95 ELISA Kit; Nonspecific cross reacting antigen NCA95 ELISA Kit
Abbreviation CEACAM8
Uniprot No. P31997
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.625 ng/mL-40 ng/mL
Sensitivity 0.156 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CEACAM8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 91  
Range % 85-96  
1:2 Average % 96  
Range % 92-103  
1:4 Average % 102  
Range % 95-108  
1:8 Average % 88  
Range % 86-91  
Recovery
The recovery of human CEACAM8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 91 86-95  
EDTA plasma (n=4) 97 94-101  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
40 2.322 2.525 2.424 2.273  
20 1.989 1.966 1.978 1.827  
10 1.365 1.481 1.423 1.272  
5 0.975 1.052 1.014 0.863  
2.5 0.603 0.625 0.614 0.463  
1.25 0.369 0.347 0.358 0.207  
0.625 0.272 0.280 0.276 0.125  
0 0.146 0.155 0.151    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Gene References into Functions
  1. Increased CD66b positive tumor-infiltrating neutrophils (CD66b + TINs) was significantly associated with presence of metastasis, S stage, and nonseminomatous germ cell tumor diagnosis. PMID: 27863478
  2. Data suggest ways in which carcinoembryonic antigen-related cell adhesion molecules CEACAM6 and CEACAM8 regulate the biological functions of one another. PMID: 26483485
  3. Granulocytes from type 2 diabetes patients display higher granulocyte cell surface expression of CD66b compared with values of healthy controls. PMID: 23686079
  4. The highly elevated gene expression of CEACAM6 and CEACAM8 in primary myelofibrosis can serve as molecular markers of myelofibrotic transformation. PMID: 21470677
  5. All the leukemic samples showed overexpression of CEACAM6 and 8 when compared with normal granulocytes. PMID: 17909799
  6. CD66b molecules are involved in regulating adhesion and activation of eosinophils, possibly through their localization in lipid rafts and interaction with other cell surface molecules, such as CD11b. PMID: 18056392

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Subcellular Location Cell membrane, Lipid-anchor, GPI-anchor
Protein Families Immunoglobulin superfamily, CEA family
Tissue Specificity Expressed in leukocytes of chronic myeloid Leukemia patients and bone marrow.
Database Links

HGNC: 1820

OMIM: 615747

KEGG: hsa:1088

STRING: 9606.ENSP00000244336

UniGene: Hs.41

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