Human Delta-like protein 4(DLL4) ELISA kit

Instructions
Code CSB-EL006949HU
Size 96T,5×96T,10×96T

Product Details

Target Name delta-like 4 (Drosophila)
Alternative Names MGC126344, hdelta2, delta 4|delta ligand 4|delta-like 4 homolog|delta-like 4 protein|notch ligand DLL4|notch ligand delta-2
Abbreviation DLL4
Uniprot No. Q9NR61
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 23.5 pg/mL-1500 pg/mL
Sensitivity 5.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Developmental Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human DLL4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %84
Range %81-89
1:2Average %94
Range %90-98
1:4Average %83
Range %80-86
1:8Average %87
Range %83-90
Recovery
The recovery of human DLL4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9591-99
EDTA plasma (n=4)10096-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
15002.261 2.298 2.280 2.098
7501.575 1.595 1.585 1.403
3750.989 0.975 0.982 0.800
187.50.643 0.606 0.625 0.443
940.434 0.429 0.432 0.250
470.317 0.338 0.328 0.146
23.50.267 0.278 0.273 0.091
00.181 0.183 0.182
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Involved in the Notch signaling pathway as Notch ligand
Involvement in disease Adams-Oliver syndrome 6 (AOS6)
Subcellular Location Cell membrane, Single-pass type I membrane protein
Tissue Specificity Expressed in vascular endothelium.
Database Links

HGNC: 2910

OMIM: 605185

KEGG: hsa:54567

STRING: 9606.ENSP00000249749

UniGene: Hs.511076

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