Human Delta-like protein 4(DLL4) ELISA kit

Code CSB-EL006949HU
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q9NR61
Lead Time 5-7 working days
Abbreviation DLL4
Protein Biological Process 1 Developmental Protein
Target Name delta-like 4 (Drosophila)
Alias MGC126344, hdelta2, delta 4|delta ligand 4|delta-like 4 homolog|delta-like 4 protein|notch ligand DLL4|notch ligand delta-2
Species Homo sapiens (Human)
Protein Biological Process 3 Differentiation
Sample Types serum, plasma, tissue homogenates
Detection Range 23.5 pg/mL-1500 pg/mL
Sensitivity 5.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Developmental Biology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene is a homolog of the Drosophila delta gene. The delta gene family encodes Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain.
HGNC 2910
RGD 1309740
MGI 1859388
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human DLL4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %84
Range %81-89
1:2Average %94
Range %90-98
1:4Average %83
Range %80-86
1:8Average %87
Range %83-90
Recovery
The recovery of human DLL4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9591-99
EDTA plasma (n=4)10096-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
15002.261 2.298 2.280 2.098
7501.575 1.595 1.585 1.403
3750.989 0.975 0.982 0.800
187.50.643 0.606 0.625 0.443
940.434 0.429 0.432 0.250
470.317 0.338 0.328 0.146
23.50.267 0.278 0.273 0.091
00.181 0.183 0.182
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Function Involved in the Notch signaling pathway as Notch ligand
Involvement in disease Adams-Oliver syndrome 6 (AOS6)
Subcellular Location Cell membrane, Single-pass type I membrane protein
Tissue Specificity Expressed in vascular endothelium.
Database Links

HGNC: 2910

OMIM: 605185

KEGG: hsa:54567

STRING: 9606.ENSP00000249749

UniGene: Hs.511076

Pathway Notch signaling pathway
Th1 and Th2 cell differentiation

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