Human E-Cadherin,E-Cad ELISA Kit

Code CSB-E04519h
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P12830
Lead Time 3-5 working days
Abbreviation CDH1
Protein Biological Process 1 Cell Adhesion
Target Name cadherin 1, type 1, E-cadherin (epithelial)
Alias Arc-1, CD324, CDHE, ECAD, LCAM, UVO, E-Cadherin|cadherin 1, E-cadherin (epithelial)|cadherin 1, type 1|calcium-dependent adhesion protein, epithelial|cell-CAM 120/80|uvomorulin
Species Homo sapiens (Human)
Protein Biological Process 3 Cell adhesion
Sample Types serum, plasma, tissue homogenates
Detection Range 6.25 ng/mL-400 ng/mL
Sensitivity 1.56 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites.
HGNC 1748
RGD 69279
MGI 88354
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human E-Cad in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %91
Range %83-99
1:2Average %96
Range %93-99
1:4Average %102
Range %97-107
1:8Average %88
Range %83-93
Recovery
The recovery of human E-Cad spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9185-97
EDTA plasma (n=4)9895-101
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
4002.290 2.399 2.345 2.204
2001.864 1.746 1.805 1.664
1001.384 1.502 1.443 1.302
500.981 1.056 1.019 0.878
250.644 0.623 0.634 0.493
12.50.389 0.379 0.384 0.243
6.250.271 0.269 0.270 0.129
00.136 0.145 0.141
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Function Cadherins are calcium-dependent cell adhesion proteins
Involvement in disease Hereditary diffuse gastric cancer (HDGC); Endometrial cancer (ENDMC); Ovarian cancer (OC); Breast cancer, lobular (LBC); Blepharocheilodontic syndrome 1 (BCDS1)
Subcellular Location Cell junction, Cell membrane, Single-pass type I membrane protein, Endosome, Golgi apparatus, trans-Golgi network
Tissue Specificity Non-neural epithelial tissues.
Database Links

HGNC: 1748

OMIM: 119580

KEGG: hsa:999

STRING: 9606.ENSP00000261769

UniGene: Hs.461086

Pathway Hippo signaling pathway
Adherens junction
Apelin signaling pathway
Rap1 signaling pathway

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