Cadherin-1 (CDH1) is also called E-cadherin (E-CD), CD antigen (CD324), uvomorulin (UVO), ECAD and CDHE. It is a member of the cadherin family, which is a calcium-dependent adhesion molecule. E-cadherin is a kind of homogenous calcium-dependent transmembrane glycoprotein that mediates cell-to-cell adhesion. Its main function is to mediate specific adhesion and plays a key role in the formation and maintenance of epithelial tissue. E-cadherin is a tumor suppressor. Studies have shown that it plays an important role in the occurrence, development, invasion and metastasis of tumor, and has become one of the important topics in the study of molecular mechanism of tumor metastasis.
The human E-CD gene is located at 16q22.1 and encodes a transmembrane glycoprotein of 882 amino acids.
Mature E-cadherin has three main domains: a cytoplasmic domain with 150 amino acid residues, a single transmembrane domain and an extracellular domain with 550 amino acids.
The extracellular region is composed of five repeating tandem structural units (CAD1-CAD5), which is a common feature of cadherin family proteins. Each unit consists of about 110 amino acid residues, that is, the extracellular part consists of 533 amino acid residues. The CAD1 region can specifically bind to Ca2+, and this region contains a conserved his-Ala-VaL (HAV) that determines the homotropism and specificity of E-CD.
The cytoplasmic region of E-CD reacts with catenin α, β and γ to form the cadherin-catenin complex (CCC). This domain is connected to the actin filament (aetin) of the cytoskeleton through catenin and participates in Wnt signal transduction.
The mutagenesis of catenin α and β plays an important role in the function of E-CD. The mutation of α-catenin gene or tyrosine phosphorylation of β-catenin can lead to the inactivation of E-CD-mediated cell adhesion system.
Epithelial cadherin exists on the surface of almost all epithelial cells and is the main type of cell-cell adhesion molecules.
In addition, E-cadherin is also widely expressed in epithelial cells of colon, uterus, liver, keratinocytes, brain, heart, muscle, kidney and pancreas, as well as erythroid cells and plasma membrane.
CDH1 is a Ca2+-dependent cell adhesion molecule. Its main function is to mediate specific adhesion between homologous cells and plays an important role in maintaining the structure and function of epithelial tissue. As a cell adhesion molecule, it is involved in development, bacterial pathogenesis and tumor invasion.
The decrease of adhesion between tumor cells is one of the important reasons for the occurrence and development of tumor. CDH1 is considered to be a tumor suppressor gene and tumor metastasis suppressor gene. The loss of CDH1 gene in tumor cells can increase the potential of invasion and metastasis of tumor cells. The low expression of E-cadherin reduces the adhesion between tumor cells, and the tumor cells are easy to fall off from the local area and then metastasize, so it is considered that E-cadherin is a tumor metastasis inhibitory factor.
CDH1 is closely related to the occurrence, development, invasion and metastasis of malignant tumors of various epithelial sources. Abnormal expression of E-cadherin was found in a variety of epithelial tumors (breast cancer, esophageal cancer, gastric cancer, oral cancer, hepatocellular carcinoma, thyroid cancer, bladder cancer and lung cancer, etc.).
It is generally believed that E-CD is a tumor metastasis inhibitory factor. Recently, a study has come up with different results. This study shows that E-cadherin is required for metastasis in multiple models of breast cancer.
The researcher tested the genetic requirement for E-cadherin in metastasis using mouse and human models of both luminal and basal invasive ductal carcinomas.
The results reveal that E-cadherin acts as a survival factor in invasive ductal carcinomas during the detachment, systemic dissemination and seeding phases of metastasis by limiting reactive oxygen-mediated apoptosis. Identifying molecular strategies to inhibit E-cadherin-mediated survival in metastatic breast cancer cells may have potential as a therapeutic approach for breast cancer.
● CDH1 Recombinant Monoclonal Antibody CSB-RA005034MA1HU
Validated Data
Positive WB detected in: Mouse Liver tissue lysate
All lanes: CDH1 antibody at 1:500
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 80-135 kDa
Observed band size: 135 kDa
IHC image of CSB-RA005034MA1HU diluted at 1:200 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
The following CDH1 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
CDH1 Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA005034GA01HU | CDH1 Antibody |
Human,Mouse,Rat | ELISA,WB,IF |
CSB-PA795479 | CDH1 Antibody |
Human,Mouse,Rat | ELISA,WB |
CSB-PA002243 | CDH1 Antibody |
Human | WB, IHC, IF, ELISA |
CSB-PA080147 | CDH1 Antibody |
Human | WB, IHC |
CSB-PA218378 | CDH1 Antibody |
Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA164023 | CDH1 Antibody |
Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA908796 | CDH1 Antibody |
Human,Mouse,Rat | ELISA,IHC |
CSB-PA938817 | CDH1 Antibody |
Human,Mouse,Rat | ELISA,IHC |
CSB-PA06239A0Rb | CDH1 Antibody |
Human | ELISA, IHC, IF |
CSB-PA06239B0Rb | CDH1 Antibody, HRP conjugated |
Human | ELISA |
CSB-PA06239C0Rb | CDH1 Antibody, FITC conjugated |
Human | |
CSB-PA06239D0Rb | CDH1 Antibody, Biotin conjugated |
Human | ELISA |
CSB-PA005034MA01HU | CDH1 Antibody |
Human | ELISA, WB, IHC, IF, IP |
CSB-PA005034MB01HU | CDH1 Antibody, HRP conjugated |
Human | ELISA |
CSB-PA005034MC01HU | CDH1 Antibody, FITC conjugated |
Human | |
CSB-PA005034MD01HU | CDH1 Antibody, Biotin conjugated |
Human | ELISA |
CSB-MA005034A0m | CDH1 Monoclonal Antibody |
Human | ELISA, WB, IF, FC |
CSB-RA576116A0HU | CDH1 Recombinant Monoclonal Antibody |
Human | ELISA, IHC |
CDH1 Antibodies for Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA347390XA01SVG | CDH1 Antibody |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | ELISA, WB (ensure identification of antigen) |
CDH1 Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-YP005034HU1 CSB-EP005034HU1 CSB-BP005034HU1 CSB-MP005034HU1 CSB-EP005034HU1-B |
Recombinant Human Cadherin-1 (CDH1) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-CF005034HU | Recombinant Human Cadherin-1 (CDH1) |
in vitro E.coli expression system |
CSB-EP005034HU | Recombinant Human Cadherin-1 (CDH1), partial |
E.coli |
CDH1 Proteins for Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Code | Product Name | Source |
---|---|---|
CSB-YP347390SVG CSB-EP347390SVG CSB-BP347390SVG CSB-MP347390SVG CSB-EP347390SVG-B |
Recombinant Saccharomyces cerevisiae APC/C activator protein CDH1 (CDH1), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CDH1 Proteins for Dictyostelium discoideum (Slime mold)
Code | Product Name | Source |
---|---|---|
CSB-YP684604DKK CSB-EP684604DKK CSB-BP684604DKK CSB-MP684604DKK CSB-EP684604DKK-B |
Recombinant Dictyostelium discoideum Anaphase-promoting complex subunit cdh1 (cdh1), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CDH1 Proteins for Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Code | Product Name | Source |
---|---|---|
CSB-YP716147PYX CSB-EP716147PYX CSB-BP716147PYX CSB-MP716147PYX CSB-EP716147PYX-B |
Recombinant Pongo abelii Cadherin-1 (CDH1), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CDH1 Proteins for Bos taurus (Bovine)
Code | Product Name | Source |
---|---|---|
CSB-YP744188BO CSB-EP744188BO CSB-BP744188BO CSB-MP744188BO CSB-EP744188BO-B |
Recombinant Bovine Cadherin-1 (CDH1), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CDH1 Proteins for Rattus norvegicus (Rat)
Code | Product Name | Source |
---|---|---|
CSB-YP889927RA CSB-EP889927RA CSB-BP889927RA CSB-MP889927RA CSB-EP889927RA-B |
Recombinant Rat Cadherin-1 (Cdh1), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CDH1 Proteins for Xenopus laevis (African clawed frog)
Code | Product Name | Source |
---|---|---|
CSB-CF005034XBE | Recombinant Xenopus laevis Cadherin-1 (cdh1) |
in vitro E.coli expression system |
CSB-YP005034XBE1 CSB-EP005034XBE1 CSB-BP005034XBE1 CSB-MP005034XBE1 CSB-EP005034XBE1-B |
Recombinant Xenopus laevis Cadherin-1 (cdh1), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CDH1 Proteins for Gallus gallus (Chicken)
Code | Product Name | Source |
---|---|---|
CSB-CF005034CH | Recombinant Chicken Cadherin-1 (CDH1) |
in vitro E.coli expression system |
CSB-YP005034CH1 CSB-EP005034CH1 CSB-BP005034CH1 CSB-MP005034CH1 CSB-EP005034CH1-B |
Recombinant Chicken Cadherin-1 (CDH1), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CDH1 Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-CF005034MO | Recombinant Mouse Cadherin-1 (Cdh1) |
in vitro E.coli expression system |
CSB-YP005034MO1 CSB-EP005034MO1 CSB-BP005034MO1 CSB-MP005034MO1 CSB-EP005034MO1-B |
Recombinant Mouse Cadherin-1 (Cdh1), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CDH1 ELISA Kit for Homo sapiens (Human)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-E04519h | Human E-Cadherin,E-Cad ELISA Kit |
serum, plasma, tissue homogenates | 0.078 ng/ml |
CDH1 ELISA Kit for Mus musculus (Mouse)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-E04520m | Mouse E-Cadherin,E-Cad ELISA Kit |
Request Information |
CDH1 ELISA Kit for Rattus norvegicus (Rat)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-E07308r | Rat Epithelial-Cadherin,E-Cad ELISA Kit |
serum, plasma, tissue homogenates, cell lysates | 0.78 ng/mL |