CDH1, a calcium-dependent cell adhesion molecule, is predominantly expressed in epithelial cells. CDH1 plays a crucial role in maintaining the structural integrity of these tissues. Its transmembrane and extracellular cadherin repeat domains enable homotypic cell adhesion, which is vital for tissue development, cell migration, proliferation control, and the maintenance of epithelial barrier function. Dysregulation of CDH1 expression is implicated in various diseases, especially cancer, where it influences tumor initiation, progression, invasion, and metastasis. As a tumor suppressor, CDH1's deficiency or dysfunction can lead to reduced cellular adhesion and increased cell mobility, promoting cells invasion and spread in cancer. The roles of CDH1 in cancer makes it a potential therapeutic target and a prognostic marker for cancers.
Recombinant Human Cadherin-1(CDH1),partial (Active) (CSB-MP005034HU1)
Validated Data
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CDH1 at 2μg/mL can bind Anti-CDH1 recombinant antibody(CSB-RA005034MA2HU),the EC50 is 5.151-6.358 ng/mL.
CDH1 Recombinant Monoclonal Antibody (CSB-RA005034MA1HU)
Validated Data
Western Blot
Positive WB detected in: Mouse Liver tissue lysate
All lanes: CDH1 antibody at 1:500
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 80-135 kDa
Observed band size: 135 kDa
IHC image of CSB-RA005034MA1HU diluted at 1:200 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
Overlay Peak curve showing MCF7 cells stained with CSB-RA005034MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,026 events was performed.
The following CDH1 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
CDH1 Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA005034GA01HU | CDH1 Antibody | Human,Mouse,Rat | ELISA,WB,IF |
CSB-PA795479 | CDH1 Antibody | Human,Mouse,Rat | ELISA,WB |
CSB-PA002243 | CDH1 Antibody | Human | WB, IHC, IF, ELISA |
CSB-PA080147 | CDH1 Antibody | Human | WB, IHC |
CSB-PA218378 | CDH1 Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA164023 | CDH1 Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA908796 | CDH1 Antibody | Human,Mouse,Rat | ELISA,IHC |
CSB-PA938817 | CDH1 Antibody | Human,Mouse,Rat | ELISA,IHC |
CSB-PA06239A0Rb | CDH1 Antibody | Human | ELISA, IHC, IF |
CSB-PA06239B0Rb | CDH1 Antibody, HRP conjugated | Human | ELISA |
CSB-PA06239C0Rb | CDH1 Antibody, FITC conjugated | Human | |
CSB-PA06239D0Rb | CDH1 Antibody, Biotin conjugated | Human | ELISA |
CSB-PA005034MA01HU | CDH1 Antibody | Human | ELISA, WB, IHC, IF, IP |
CSB-PA005034MB01HU | CDH1 Antibody, HRP conjugated | Human | ELISA |
CSB-PA005034MC01HU | CDH1 Antibody, FITC conjugated | Human | |
CSB-PA005034MD01HU | CDH1 Antibody, Biotin conjugated | Human | ELISA |
CSB-MA005034A0m | CDH1 Monoclonal Antibody | Human | ELISA, WB, IF, FC |
CSB-RA576116A0HU | CDH1 Recombinant Monoclonal Antibody | Human | ELISA, IHC |
CSB-MA196808 | CDH1 Monoclonal Antibody | Human | ELISA, IHC |
CSB-RA005034MA1HU | CDH1 Recombinant Monoclonal Antibody | Human, Mouse | ELISA, WB, IHC, FC |
CDH1 Antibodies for Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA347390XA01SVG | CDH1 Antibody | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | ELISA, WB (ensure identification of antigen) |
CDH1 Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-YP005034HU1 CSB-EP005034HU1 CSB-BP005034HU1 CSB-MP005034HU1 CSB-EP005034HU1-B |
Recombinant Human Cadherin-1 (CDH1) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-CF005034HU | Recombinant Human Cadherin-1 (CDH1) | in vitro E.coli expression system |
CSB-EP005034HU | Recombinant Human Cadherin-1 (CDH1), partial | E.coli |
CSB-MP005034HU1 | Recombinant Human Cadherin-1(CDH1),partial (Active) | Mammalian cell |
CDH1 Proteins for Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Code | Product Name | Source |
---|---|---|
CSB-YP347390SVG CSB-EP347390SVG CSB-BP347390SVG CSB-MP347390SVG CSB-EP347390SVG-B |
Recombinant Saccharomyces cerevisiae APC/C activator protein CDH1 (CDH1), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CDH1 Proteins for Dictyostelium discoideum (Slime mold)
Code | Product Name | Source |
---|---|---|
CSB-YP684604DKK CSB-EP684604DKK CSB-BP684604DKK CSB-MP684604DKK CSB-EP684604DKK-B |
Recombinant Dictyostelium discoideum Anaphase-promoting complex subunit cdh1 (cdh1), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CDH1 Proteins for Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Code | Product Name | Source |
---|---|---|
CSB-YP716147PYX CSB-EP716147PYX CSB-BP716147PYX CSB-MP716147PYX CSB-EP716147PYX-B |
Recombinant Pongo abelii Cadherin-1 (CDH1), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CDH1 Proteins for Bos taurus (Bovine)
Code | Product Name | Source |
---|---|---|
CSB-YP744188BO CSB-EP744188BO CSB-BP744188BO CSB-MP744188BO CSB-EP744188BO-B |
Recombinant Bovine Cadherin-1 (CDH1), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CDH1 Proteins for Rattus norvegicus (Rat)
Code | Product Name | Source |
---|---|---|
CSB-YP889927RA CSB-EP889927RA CSB-BP889927RA CSB-MP889927RA CSB-EP889927RA-B |
Recombinant Rat Cadherin-1 (Cdh1), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CDH1 Proteins for Xenopus laevis (African clawed frog)
Code | Product Name | Source |
---|---|---|
CSB-CF005034XBE | Recombinant Xenopus laevis Cadherin-1 (cdh1) | in vitro E.coli expression system |
CSB-YP005034XBE1 CSB-EP005034XBE1 CSB-BP005034XBE1 CSB-MP005034XBE1 CSB-EP005034XBE1-B |
Recombinant Xenopus laevis Cadherin-1 (cdh1), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CDH1 Proteins for Gallus gallus (Chicken)
Code | Product Name | Source |
---|---|---|
CSB-CF005034CH | Recombinant Chicken Cadherin-1 (CDH1) | in vitro E.coli expression system |
CSB-YP005034CH1 CSB-EP005034CH1 CSB-BP005034CH1 CSB-MP005034CH1 CSB-EP005034CH1-B |
Recombinant Chicken Cadherin-1 (CDH1), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CDH1 Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-CF005034MO | Recombinant Mouse Cadherin-1 (Cdh1) | in vitro E.coli expression system |
CSB-YP005034MO1 CSB-EP005034MO1 CSB-BP005034MO1 CSB-MP005034MO1 CSB-EP005034MO1-B |
Recombinant Mouse Cadherin-1 (Cdh1), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CDH1 ELISA Kit for Homo sapiens (Human)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-E04519h | Human E-Cadherin,E-Cad ELISA Kit | serum, plasma, tissue homogenates | 0.078 ng/ml |
CDH1 ELISA Kit for Mus musculus (Mouse)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-E04520m | Mouse E-Cadherin,E-Cad ELISA Kit | Request Information |
CDH1 ELISA Kit for Rattus norvegicus (Rat)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-E07308r | Rat Epithelial-Cadherin,E-Cad ELISA Kit | serum, plasma, tissue homogenates, cell lysates | 0.78 ng/mL |