Product Details

Target Name

glutaminyl-peptide cyclotransferase

Alternative Names

5730422A13Rik ELISA Kit; EC ELISA Kit; GCT ELISA Kit; Glutaminyl cyclase ELISA Kit; Glutaminyl peptide cyclotransferase ELISA Kit; Glutaminyl tRNA cyclotransferase ELISA Kit; Glutaminyl-peptide cyclotransferase ELISA Kit; Glutaminyl-tRNA cyclotransferase ELISA Kit; Glutamyl cyclase ELISA Kit; QC ELISA Kit; qpct ELISA Kit; QPCT_HUMAN ELISA Kit; sQC ELISA Kit

Abbreviation

QPCT

Uniprot No.

Q16769

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.312 ng/mL-20 ng/mL

Sensitivity

0.078 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human QPCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-100
1:2	Average %	103
	Range %	99-105
1:4	Average %	93
	Range %	87-97
1:8	Average %	91
	Range %	89-98

Recovery

The recovery of human QPCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	92-98
EDTA plasma (n=4)	90	86-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	1.846	1.775	1.811	1.676
10	1.469	1.466	1.468	1.333
5	1.156	1.244	1.200	1.065
2.5	0.878	0.854	0.866	0.731
1.25	0.583	0.623	0.603	0.468
0.625	0.374	0.388	0.381	0.246
0.312	0.242	0.251	0.247	0.112
0	0.134	0.135	0.135

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human QPCT ELISA Kit was designed for the quantitative measurement of Human QPCT protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

Related Products

Target Background

Function
(From Uniprot)

Responsible for the biosynthesis of pyroglutamyl peptides. Has a bias against acidic and tryptophan residues adjacent to the N-terminal glutaminyl residue and a lack of importance of chain length after the second residue. Also catalyzes N-terminal pyroglutamate formation. In vitro, catalyzes pyroglutamate formation of N-terminally truncated form of APP amyloid-beta peptides [Glu-3]-amyloid-beta. May be involved in the N-terminal pyroglutamate formation of several amyloid-related plaque-forming peptides.

Gene References into Functions

The addition of QC activity to core diagnostic CSF biomarkers may be of specific interest in clinical cases with discordant imaging and biochemical biomarker results. PMID: 28587659
these findings demonstrate a significant association between common single nucleotide polymorphism within QPCT gene and schizophrenia risk in a Han Chinese population. PMID: 26572640
the genetic risk of QPCT gene for schizophrenia also exists in the Han Chinese population. PMID: 26492838
observations provide evidence for an involvement of QC in Alzheimer's disease pathogenesis and cognitive decline by QC-catalyzed pGlu-Abeta formation PMID: 24164736
this study demonistrated that QPCT mRNAand protein from mononuclear cells correlated with theseverity of dementia. PMID: 23207485
Upregulation of QPCT expression is associated with thyroid carcinomas. PMID: 23183267
This study presents a first comparison of two mammalian QCs (human and mouse) containing typical, conserved post-translational modifications. PMID: 21671571
The resilts of this studt provided histopathological evidence for QC being a prerequisite for pE-Abeta pathology in vivo and further underline the therapeutic potential of QC inhibition in Alzheimer Disease. PMID: 21301857
Upon binding to PBD150, a large loop movement in gQC allows the inhibitor to be tightly held in its active site primarily by hydrophobic interactions. PMID: 21288892
This study demonstrated that glutaminyl cyclase expression and pE-Abeta formation in subcortical brain regions( Edinger-Westphal nucleus, locus coeruleus and nucleus basalis Meynert) affected in Alzheimer's disease. PMID: 20383514
Human isoQC proteins displayed a broad substrate specificity and preference for hydrophobic substrates, similar to the related QC. PMID: 19804409
Functional expression of human glutaminyl cyclase in Pichia pastoris reveals the presence of a disulfide bond with an important role in the stabilization of active protein structure. PMID: 12196024
human glutaminyl cyclase is a metal-dependent transferase PMID: 14522962
Glutaminyl cyclase was expressed as a fully active 37 kDa enzyme with kcat/Km values of 14.3, 9.3, and 2.4 mM(-1)s(-1) for the substrates Gln-Gln, Gln-NH(2), and Gln-t-butyl ester, respectively PMID: 14680951
Genetic variations in QPCT are important factors affecting the bone mineral density of adult women that contribute to susceptibility for osteoporosis. PMID: 15231017
structure of human QC in free form and bound to a substrate and three imidazole-derived inhibitors PMID: 16135565
Our results suggest that QPCT may be the QTL gene at chromosome 2p for spine BMD variation in the Chinese population PMID: 17687619
combined steady-state enzyme kinetic and X-ray structural analyses of 11 single-mutation human QCs to investigate the roles of the H-bond network in catalysis PMID: 18072935
The computations outline the important role of Trp329 in helping the substrate binding process and stabilizing the cyclization transition state. PMID: 18470930
Golgi apparatus retention implies a "housekeeping" protein maturation machinery conducting glycosylation and pyroglutamyl formation. For these isoenzymes, apparently similar strategies evolved to retain the proteins in the Golgi complex. PMID: 18486145

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Subcellular Location

Secreted.

Protein Families

Glutaminyl-peptide cyclotransferase family

Database Links

HGNC: 9753

OMIM: 607065

KEGG: hsa:25797

STRING: 9606.ENSP00000344829

UniGene: Hs.79033

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Code	CSB-EL019135HU
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Human Glutaminyl-peptide cyclotransferase(QPCT) ELISA kit

Product Details

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Target Background