Human Hyaluronidase PH-20(SPAM1) ELISA kit

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Human Hyaluronidase PH-20(SPAM1) ELISA kit

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Code	CSB-EL022471HU-IS
Size	96T,5×96T,10×96T
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Target Background

Target Name

sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding)

Alternative Names

epididymis secretory sperm binding protein Li 96n ELISA Kit; HEL-S-96n ELISA Kit; HYA1 ELISA Kit; HYAL PH-20 ELISA Kit; HYAL PH20 ELISA Kit; Hyal-PH20 ELISA Kit; HYAL1 ELISA Kit; HYAL3 ELISA Kit; HYAL5 ELISA Kit; HYALP_HUMAN ELISA Kit; Hyaluronidase PH-20 ELISA Kit; Hyaluronoglucosaminidase PH-20 ELISA Kit; MGC26532 ELISA Kit; PH-20 Hyaluronidase ELISA Kit; PH20 ELISA Kit; PH20 Hyaluronidase ELISA Kit; SPAG15 ELISA Kit; SPAM-1 ELISA Kit; Spam1 ELISA Kit; sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) ELISA Kit; Sperm adhesion molecule 1 ELISA Kit; Sperm surface protein PH-20 ELISA Kit; Sperm surface protein PH20 ELISA Kit; zona pellucida binding ELISA Kit

Abbreviation

SPAM1

Uniprot No.

P38567

Species

Homo sapiens (Human)

Sample Types

cell culture supernates

Detection Range

78 pg/mL-5000 pg/mL

Sensitivity

39 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human SPAM1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Cell Culture Supernates(n=4)
1:400	Average %	87
	Range %	81-95
1:800	Average %	93
	Range %	89-100
1:1600	Average %	106
	Range %	99-110
1:3200	Average %	90
	Range %	82-93

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
5000	3.654	3.670	3.662	3.576
2500	3.017	3.123	3.070	2.984
1250	2.138	2.192	2.165	2.079
625	1.137	1.194	1.166	1.080
312	0.684	0.708	0.696	0.610
156	0.418	0.439	0.428	0.343
78	0.226	0.220	0.223	0.137
0	0.085	0.087	0.086

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function
(From Uniprot)

Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.

Gene References into Functions

Human posterior head 20 (hPH20) and homo sapiens sperm acrosome associated 1 (hSPACA1) immunocontraceptive epitopes reduced fertility in male/female mice. PMID: 25209425
results indicate that neither rHuPH20 nor its directly generated HA catabolites have inflammatory properties in the air pouch model, and rHuPH20 can instead inhibit some aspects of inflammation, such as neutrophil infiltration into the air pouch. PMID: 24778442
HSPA2 regulates the expression of sperm surface receptors involved in human sperm-oocyte recognition, such as arylsulfatase A and SPAM1. PMID: 23247813
The interaction between SPAM1, ARSA and HSPA2 in a multimeric complex mediating sperm-egg interaction. PMID: 23209833
PH20 is elevated in demyelinating lesions and that increased PH20 expression is sufficient to inhibit oligodendrocyte precursor cell maturation and remyelination. PMID: 23463525
SPAM1 mRNA & protein occur in all 3 regions of epididymis & vas deferens. It has hyaluronidase activity at pH 7.0. The proximal promoter has epididymal transcription factor sites including androgen receptor elements. Its role may be in sperm maturation. PMID: 12932297

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Subcellular Location

Cell membrane; Lipid-anchor, GPI-anchor.

Protein Families

Glycosyl hydrolase 56 family

Tissue Specificity

Testis.

Database Links

HGNC: 11217

OMIM: 600930

KEGG: hsa:6677

STRING: 9606.ENSP00000345849

UniGene: Hs.121494

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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