Human Hyaluronidase PH-20(SPAM1) ELISA kit

Product Details

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Target Background

Product Details

Target Name

sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding)

Associated names

epididymis secretory sperm binding protein Li 96n ELISA Kit; HEL-S-96n ELISA Kit; HYA1 ELISA Kit; HYAL PH-20 ELISA Kit; HYAL PH20 ELISA Kit; Hyal-PH20 ELISA Kit; HYAL1 ELISA Kit; HYAL3 ELISA Kit; HYAL5 ELISA Kit; HYALP_HUMAN ELISA Kit; Hyaluronidase PH-20 ELISA Kit; Hyaluronoglucosaminidase PH-20 ELISA Kit; MGC26532 ELISA Kit; PH-20 Hyaluronidase ELISA Kit; PH20 ELISA Kit; PH20 Hyaluronidase ELISA Kit; SPAG15 ELISA Kit; SPAM-1 ELISA Kit; Spam1 ELISA Kit; sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) ELISA Kit; Sperm adhesion molecule 1 ELISA Kit; Sperm surface protein PH-20 ELISA Kit; Sperm surface protein PH20 ELISA Kit; zona pellucida binding ELISA Kit

Abbreviation

SPAM1

Uniprot No.

P38567

Species

Homo sapiens (Human)

Sample Types

cell culture supernates

Detection Range

78 pg/mL-5000 pg/mL

Sensitivity

39 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human SPAM1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Cell Culture Supernates(n=4)
1:400	Average %	87
	Range %	81-95
1:800	Average %	93
	Range %	89-100
1:1600	Average %	106
	Range %	99-110
1:3200	Average %	90
	Range %	82-93

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
5000	3.654	3.670	3.662	3.576
2500	3.017	3.123	3.070	2.984
1250	2.138	2.192	2.165	2.079
625	1.137	1.194	1.166	1.080
312	0.684	0.708	0.696	0.610
156	0.418	0.439	0.428	0.343
78	0.226	0.220	0.223	0.137
0	0.085	0.087	0.086

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This ELISA kit provides quantitative measurement of human SPAM1 (sperm adhesion molecule 1/hyaluronidase PH-20) in research contexts spanning signal transduction and reproductive biology. Because SPAM1 is a GPI-anchored hyaluronidase that can be released from cell surfaces into conditioned media, detecting its soluble form at low concentrations demands purpose-built assay sensitivity.

Detection Range: The 78–5000 pg/mL range captures the low-to-moderate concentrations of shed SPAM1 typically found in cell culture supernates from transfected lines or primary reproductive tract cells, minimizing the need for extensive sample concentration while accommodating higher-expression systems through simple dilution.

Sensitivity: At 39 pg/mL, the kit detects SPAM1 in conditioned media from cells with modest endogenous expression, including epididymal or cumulus cell cultures where soluble PH-20 levels may be near the lower physiological threshold.

Sample Compatibility: Validation for cell culture supernates directly supports in vitro studies of SPAM1 shedding mechanisms, GPI-anchor cleavage regulation, and hyaluronidase activity modulation without requiring cell lysis or membrane fractionation.

Assay Efficiency: The 1–5 hour protocol and 50–100 µL sample volume accommodate experiments where conditioned media volumes are limited, such as small-scale primary spermatocyte or organoid cultures, enabling same-day quantification across multiple treatment conditions.

This kit is well suited for studies of sperm-oocyte interaction, cumulus matrix remodeling, and SPAM1-mediated signaling in fertilization research.

Code	CSB-EL022471HU-IS
Size	96T,5×96T,10×96T
Price	Request a Quote
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.