Human Inosine-5'-monophosphate dehydrogenase 2 (IMPDH2) ELISA kit

Code CSB-E17855h
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
IMP (inosine monophosphate) dehydrogenase 2
Alternative Names
IMDH2_HUMAN ELISA Kit; IMP (inosine monophosphate) dehydrogenase 2 ELISA Kit; IMP dehydrogenase 2 ELISA Kit; IMP oxireductase 2 ELISA Kit; IMPD 2 ELISA Kit; IMPD2 ELISA Kit; IMPDH 2 ELISA Kit; IMPDH II ELISA Kit; IMPDH-II ELISA Kit; Impdh2 ELISA Kit; Inosine 5' monophosphate dehydrogenase 2 ELISA Kit; Inosine 5' phosphate dehydrogenase 2 ELISA Kit; Inosine monophosphate dehydrogenase type II ELISA Kit; Inosine-5''-monophosphate dehydrogenase 2 ELISA Kit
Abbreviation
IMPDH2
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
23.5 pg/mL-1500 pg/mL
Sensitivity
5.8 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human IMPDH2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 94  
Range % 88-98  
1:2 Average % 97  
Range % 93-102  
1:4 Average % 94  
Range % 89-98  
1:8 Average % 87  
Range % 82-91  
Recovery
The recovery of human IMPDH2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 98 94-102  
EDTA plasma (n=4) 88 82-92  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1500 2.632 2.510 2.571 2.448  
750 1.476 1.519 1.498 1.375  
375 0.867 0.820 0.844 0.721  
187.5 0.473 0.465 0.469 0.346  
94 0.305 0.311 0.308 0.185  
47 0.258 0.246 0.252 0.129  
23.5 0.189 0.185 0.187 0.064  
0 0.124 0.122 0.123    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human IMPDH2 ELISA Kit was designed for the quantitative measurement of Human IMPDH2 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL.

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Target Background

Function
(From Uniprot)
Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism. It may also have a role in the development of malignancy and the growth progression of some tumors.
Gene References into Functions
  1. Our study demonstrates IMPDH2 may be served as an independent prognostic biomarker for NPC patients, in which high IMPDH expression suggests poor prognosis of NPC patients. PMID: 28389646
  2. inosine-5'-monophosphate dehydrogenase 2 (IMPDH2) is an intracellular target of the PPIA-Sanglifehrin A binary complex. PMID: 28076787
  3. Rod and ring formation might be an adaptive homeostatic mechanism, allowing IMPDH to sense changes in the one-carbon folate pathway. PMID: 27343244
  4. Taking together, these results indicate that natural product myricetin exhibits potent anti-leukemia activity by interfering with purine nucleotides biosynthetic pathway through the suppression of hIMPDH1/2 catalytic activity. PMID: 27378425
  5. The findings suggest that overexpressed IMPDH2 can be used as a biomarker for kidney and bladder cancer diagnosis and is a potential therapeutic target for the diseases PMID: 25465060
  6. IMPDH2 is directly involved in the development of chemoresistance and radioresistance in osteosarcoma cells. PMID: 25392102
  7. Compared with non-cancerous prostate tissues, IMPDH2 mRNA and protein expression levels were both significantly upregulated in prostate cancer tissues. PMID: 24659377
  8. the localization of IMPDH2 inside the nucleus of human cells as well as the ultrastructure of R&R inclusions PMID: 24980853
  9. Rods and rings (RR) are protein assemblies composed of CTPS1 and IMPDH2. Glutamine deprivation initiates reversible assembly of mammalian rods and rings. PMID: 24477477
  10. An insight to the dynamics of conserved water-mediated salt bridge interaction and interdomain recognition in hIMPDH isoforms. PMID: 22928911
  11. Role of salt bridge dynamics in inter domain recognition of human IMPDH isoforms: an insight to inhibitor topology for isoform-II. PMID: 22066532
  12. This ethnic-related data might contribute to a better understanding of the variability in clinical outcome and/or dose adjustments of drugs that are IMPDH inhibitors such as mycophenolic acid. PMID: 21181270
  13. the 3757C allele was associated with higher lymphocyte counts and a reduced incidence of lymphopenia among kidney allograft recipients PMID: 21996196
  14. Anti-IMPDH2 may cause difficulties in interpretation of immunofluorescence patterns in routine autoantibody testing. PMID: 22029192
  15. IMPDHII plays an important role in the negative regulation of TLR2 signaling by modulating PI3K activity. PMID: 21460227
  16. in the absence of ligand the mobilities of "flap" and "loop" regions of IMPDH-II (1B3O) structures are mostly controlled by the four (W 1, W 2, W 3, and W 4) conserved water molecular centers and their dynamics PMID: 20135634
  17. resequenced IMPDH1 and IMPDH2 using DNA from 288 individuals from three ethnic groups and performed functional genomic studies of the sequence variants observed; identfied 25 SNPs, 24 novel, in IMPDH2 PMID: 20718729
  18. IMPDH2 is directly involved in the development of chemoresistance in osteosarcoma cells PMID: 20808934
  19. IMPDH2 genetic polymorphism was investigated in 96 individuals of Caucasian origin. PMID: 19810816
  20. Pharmacogenetic analysis of IMPDH II may represent a novel approach to mycophenolate mofetil therapeutic monitoring. PMID: 15621150
  21. Nine genetic variants were identified in the IMPDH2 gene, with frequencies of the rarer alleles ranging from 0.5 to 10.2%. PMID: 17496727
  22. IMPDH2 is a marker gene useful for stratifying osteosarcoma patients into low- and high-risk groups and predicting therapy outcome. PMID: 17660802
  23. The effect of diabetes mellitus on mycophenolic acid pharmacokinetics and catalytic activity of inosine monophosphate dehydrogenase 2 was investigated in maintenance kidney transplant recipients. PMID: 18043470
  24. The synthesis and activity of several novel inhibitors of IMPDH2 are reported. PMID: 18583139
  25. Analysis of in vivo C-MYC interactions with TS, IMPDH2 and PRPS2 genes confirmed that they are direct C-MYC targets. PMID: 18677108
  26. The expression of IMPDH2 in tumor tissue was significantly higher in patients with colorectal cancer than that in healthy subjects. PMID: 19597826
  27. the IMPDH type II 3757T > C polymorphism is associated with an increased IMPDH activity in MMF-treated renal transplant patients PMID: 19617864

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Subcellular Location
Cytoplasm. Nucleus. Cytoplasm, cytosol.
Protein Families
IMPDH/GMPR family
Tissue Specificity
IMPDH1 is the main species in normal leukocytes and IMPDH2 predominates over IMPDH1 in the tumor.
Database Links

HGNC: 6053

OMIM: 146691

KEGG: hsa:3615

STRING: 9606.ENSP00000321584

UniGene: Hs.654400

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