Code | CSB-E11707h |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of human IL-21 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 90 | ||||
Range % | 85-96 | |||||
1:2 | Average % | 92 | ||||
Range % | 87-99 | |||||
1:4 | Average % | 87 | ||||
Range % | 84-92 | |||||
1:8 | Average % | 94 | ||||
Range % | 87-100 |
The recovery of human IL-21 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 98 | 94-102 | ||||
EDTA plasma (n=4) | 101 | 85-109 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This human IL-21 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human IL-21 in multiple samples, including serum, plasma, or tissue homogenates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the IL-21 levels in the sample and is measured at 450 nm through a microplate reader.
IL-21 plays multiple biologic roles, including maintenance and function of CD8+ memory T cells and natural killer cells, as well as promoting the generation of Th17 cells in the mouse. IL-21 also promotes B-cell activation, differentiation, or death during humoral immune responses. IL-21 signals through IL-21R, activating the JAK1/3-STAT signaling pathway that initiates a gene transcription profile such as Gzma, Gzmb, Il10, Eomes, and Rorgt. Furthermore, elevated IL-21 generation is characteristic of certain autoimmune diseases and is likely to contribute to autoantibody production as well as pathologic features of autoimmune disease. In contrast, IL-21 may function as a co-adjuvant to enhance antibody responses and thereby facilitate host defense to malignancies and infectious diseases.
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