The product CSB-E13418h is an in vitro ELISA kit that quantitatively measures the IL-22 levels in human serum, plasma, or tissue homogenates. IL-22 is an IL-10-family cytokine generated by Th17 cells, γδ T cells, and type 3 innate lymphoid cells (ILCs). Unlike other homologs, IL-22 only works on non-hematopoietic cells such as epithelial cells and stromal cells in mucosal tissues. IL-22 mainly targets epithelial cells and is involved in epithelial regeneration and barrier function, including regulation of epithelial cell growth and permeability, production of mucus and antimicrobial proteins (AMPs), and complement production. IL-22 signaling via IL-22Ra1-STAT3 plays a crucial role in regulating mucosal immunity, microbiota colonization, inflammation, and tissue repair.
This assay employs the sandwich ELISA technique in which IL-22 in the sample is sandwiched between the pre-coated anti-human IL-22 antibody and Biotin-labeled IL-22 antibody and the Ag-Ab-Ag complex is labeled by the HRP-avidin and then develops color reaction after the addition of the TMB substrate solution. The color intensity can be measured by a microplate reader at 450 nm. The kit has been validated with high sensitivity, excellent specificity, precision low than 10%, high recovery, and consistency between batches.