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Human Interleukin 31(IL-31) ELISA Kit

Human Interleukin 31(IL-31) ELISA Kit

Specification
Target

Code

CSB-E16999h

See More Details

Product Type

ELISA Kit

Size

96T,5×96T,10×96T

Uniprot No.

Q6EBC2

Lead Time

3-5 working days

Abbreviation

IL31

Protein Biological Process 1

Immunity

Target Name

interleukin 31

Alias

IL-31,

Species

Homo sapiens (Human)

Protein Biological Process 3

Immunity

Sample Types

serum, plasma

Detection Range

125 pg/mL-8000 pg/mL

Sensitivity

31.25 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Protocol

Instructions

Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.

Assay Principle

quantitative

Measurement

Sandwich

HGNC

19372

MGI

1923649

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IL-31 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	84-96
1:2	Average %	96
	Range %	90-102
1:4	Average %	84
	Range %	81-87
1:8	Average %	88
	Range %	83-94

Recovery

The recovery of human IL-31 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	96-105
EDTA plasma (n=4)	107	100-114

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
8000	1.774	1.811	1.793	1.627
4000	1.003	1.055	1.029	0.863
2000	0.666	0.687	0.677	0.511
1000	0.401	0.412	0.407	0.241
500	0.289	0.302	0.296	0.130
250	0.245	0.255	0.250	0.084
125	0.211	0.216	0.214	0.048
0	0.163	0.168	0.166

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Function	Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR
Subcellular Location	Secreted
Tissue Specificity	Detected at low levels in testis, bone marrow, skeletal muscle, kidney, colon, thymus, small intestine and trachea.
Database Links	HGNC: 19372 OMIM: 609509 KEGG: hsa:386653 STRING: 9606.ENSP00000366234 UniGene: Hs.569071

Related Products

IL31 Antibody

IL31 Protein

IL31 Antibodies

IL31 Antibodies for Homo sapiens (Human)

Code	Product Name	Species Reactivity	Application
CSB-PA930930	IL31 Antibody	Human	ELISA,IHC
CSB-PA239746	IL31 Antibody	Human	ELISA,WB,IHC
CSB-PA723018	IL31 Antibody	Human	WB,ELISA

IL31 Proteins

IL31 Proteins for Homo sapiens (Human)

Code	Product Name	Source
CSB-AP004631HU	Recombinant Human Interleukin-31(IL31) (Active)	E.coli
CSB-BP735356HU	Recombinant Human Interleukin-31(IL31)	Baculovirus
CSB-YP735356HU CSB-MP735356HU	Recombinant Human Interleukin-31(IL31)	Yeast Mammalian cell
CSB-EP735356HU	Recombinant Human Interleukin-31(IL31)	E.coli
CSB-AP001901HU	Recombinant Human Interleukin-31 protein(IL31) (Active)	E.coli

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Human Interleukin 31(IL-31) ELISA Kit

Specification

Target

Related Products

IL31 Antibodies

IL31 Proteins

Most popular with customers

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