Human Interleukin 31(IL-31) ELISA Kit

Code CSB-E16999h
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q6EBC2
Lead Time 3-5 working days
Abbreviation IL31
Protein Biological Process 1 Immunity
Target Name interleukin 31
Alias IL-31,
Species Homo sapiens (Human)
Protein Biological Process 3 Immunity
Sample Types serum, plasma
Detection Range 125 pg/mL-8000 pg/mL
Sensitivity 31.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
HGNC 19372
MGI 1923649
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human IL-31 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 88
Range % 84-96
1:2 Average % 96
Range % 90-102
1:4 Average % 84
Range % 81-87
1:8 Average % 88
Range % 83-94
Recovery
The recovery of human IL-31 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 101 96-105
EDTA plasma (n=4) 107 100-114
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
8000 1.774 1.811 1.793 1.627
4000 1.003 1.055 1.029 0.863
2000 0.666 0.687 0.677 0.511
1000 0.401 0.412 0.407 0.241
500 0.289 0.302 0.296 0.130
250 0.245 0.255 0.250 0.084
125 0.211 0.216 0.214 0.048
0 0.163 0.168 0.166  
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Function Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR
Subcellular Location Secreted
Tissue Specificity Detected at low levels in testis, bone marrow, skeletal muscle, kidney, colon, thymus, small intestine and trachea.
Database Links

HGNC: 19372

OMIM: 609509

KEGG: hsa:386653

STRING: 9606.ENSP00000366234

UniGene: Hs.569071

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