Code | CSB-E16999h |
Size | 96T,5×96T,10×96T |
Price | Request a Quote |
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Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >> Interested in a trial size? Please leave a message below.
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Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. |
To assess the linearity of the assay, samples were spiked with high concentrations of human IL-31 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | ||||
1:1 | Average % | 88 | ||||
Range % | 84-96 | |||||
1:2 | Average % | 96 | ||||
Range % | 90-102 | |||||
1:4 | Average % | 84 | ||||
Range % | 81-87 | |||||
1:8 | Average % | 88 | ||||
Range % | 83-94 |
The recovery of human IL-31 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 101 | 96-105 | ||||
EDTA plasma (n=4) | 107 | 100-114 |
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
pg/ml | OD1 | OD2 | Average | Corrected | |||
8000 | 1.774 | 1.811 | 1.793 | 1.627 | |||
4000 | 1.003 | 1.055 | 1.029 | 0.863 | |||
2000 | 0.666 | 0.687 | 0.677 | 0.511 | |||
1000 | 0.401 | 0.412 | 0.407 | 0.241 | |||
500 | 0.289 | 0.302 | 0.296 | 0.130 | |||
250 | 0.245 | 0.255 | 0.250 | 0.084 | |||
125 | 0.211 | 0.216 | 0.214 | 0.048 | |||
0 | 0.163 | 0.168 | 0.166 |
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This human IL-31 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human IL-31 in multiple samples, including serum, plasma, or tissue homogenates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the IL-31 levels in the sample and is measured at 450 nm through a microplate reader.
IL-31 signaling controls a wide range of biological functions and immunomodulatory effects, such as the release of chemokines, proinflammatory cytokines, modulation of cell proliferation, and stimulation of dorsal root ganglia (DRG) sensory neurons which are responsible for the induction of itch. Alongside its pruritogenic actions, IL-31 has a proinflammatory role due to the upregulation of proinflammatory gene expressions in T cells, including CCL2 and granulocyte colony-stimulating factor. Elevated levels of IL-31 have now been found in various inflammatory skin diseases including prurigo nodularis, atopic dermatitis, contact eczema, chronic spontaneous urticaria (CsU), as well as in a subset of patients with mastocytosis.
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