Human Interleukin-7,IL-7 ELISA kit

Code CSB-E14032h
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P13232
Lead Time 3-5 working days
Abbreviation IL7
Target Name interleukin 7
Alias IL-7, IL7 nirs variant 6
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 0.78 pg/mL-50 pg/mL
Sensitivity 0.195 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival.
HGNC 6023
RGD 2904
MGI 96561
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human IL-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 93    
Range % 88-98    
1:2 Average % 97    
Range % 91-102    
1:4 Average % 96    
Range % 92-101    
1:8 Average % 89    
Range % 83-94    
Recovery
The recovery of human IL-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 102 98-105    
EDTA plasma (n=4) 96 87-99    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
50 2.467 2.350 2.409 2.266    
25 1.782 1.652 1.717 1.574    
12.5 1.186 1.102 1.144 1.001    
6.25 0.677 0.694 0.686 0.543    
3.12 0.458 0.474 0.466 0.323    
1.56 0.334 0.327 0.331 0.188    
0.78 0.218 0.212 0.215 0.072    
0 0.144 0.142 0.143      
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Function Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation.
Subcellular Location Secreted
Protein Families IL-7/IL-9 family
Database Links

HGNC: 6023

OMIM: 146660

KEGG: hsa:3574

STRING: 9606.ENSP00000263851

UniGene: Hs.591873

Pathway Jak-STAT signaling pathway
PI3K-Akt signaling pathway
Hematopoietic cell lineage

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