Human Interleukin17F (IL17F) ELISA Kit

Code CSB-E15916h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q96PD4
Lead Time 3-5 working days
Abbreviation IL17F
Target Name interleukin 17F
Alias IL-17F, ML-1, ML1, cytokine ML-1
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates
Detection Range 12.5 pg/mL-800 pg/mL
Sensitivity 3.1 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1.
HGNC 16404
RGD 1311398
MGI 2676631
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human IL17F in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %93
Range %87-99
1:2Average %103
Range %99-108
1:4Average %80
Range %85-96
1:8Average %93
Range %85-98
Recovery
The recovery of human IL17F spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9287-96
EDTA plasma (n=4)8985-93
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
8001.850 1.920 1.885 1.795
4001.342 1.405 1.374 1.284
2000.834 0.902 0.868 0.778
1000.557 0.525 0.541 0.451
500.325 0.342 0.334 0.244
250.253 0.264 0.259 0.169
12.50.146 0.150 0.148 0.058
00.087 0.092 0.090  
References
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Function Ligand for IL17RA and IL17RC
Involvement in disease Candidiasis, familial, 6 (CANDF6)
Subcellular Location Secreted
Protein Families IL-17 family
Tissue Specificity Expressed in activated, but not resting, CD4+ T-cells and activated monocytes.
Database Links

HGNC: 16404

OMIM: 606496

KEGG: hsa:112744

STRING: 9606.ENSP00000337432

UniGene: Hs.272295

Pathway IL-17 signaling pathway
Th17 cell differentiation

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