Human Melanotransferrin(MFI2) ELISA kit

Code CSB-EL013754HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5
Alternative Names
Antigen p97 ELISA Kit; CD228 ELISA Kit; CD228 antigen ELISA Kit; MAP97 ELISA Kit; Melanoma associated antigen p97 ELISA Kit; Melanoma-associated antigen p97 ELISA Kit; Melanotransferrin ELISA Kit; Mfi2 ELISA Kit; MTF1 ELISA Kit; TRFM_HUMAN ELISA Kit
Abbreviation
MFI2
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
0.156 ng/mL-10 ng/mL
Sensitivity
0.039 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human MFI2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %97
Range %92-101
1:2Average %103
Range %99-108
1:4Average %90
Range %85-94
1:8Average %92
Range %88-96
Recovery
The recovery of human MFI2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8984-93
EDTA plasma (n=4)9590-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1OD2AverageCorrected
102.486 2.315 2.401 2.296
52.034 1.974 2.004 1.899
2.51.506 1.414 1.460 1.355
1.251.027 0.981 1.004 0.899
0.6250.631 0.663 0.647 0.542
0.3120.348 0.325 0.337 0.232
0.1560.241 0.257 0.249 0.144
00.107 0.103 0.105
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human MFI2 ELISA Kit was designed for the quantitative measurement of Human MFI2 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

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Target Background

Function
(From Uniprot)
Involved in iron cellular uptake. Seems to be internalized and then recycled back to the cell membrane. Binds a single atom of iron per subunit. Could also bind zinc.
Gene References into Functions
  1. Melanotransferrin is a serological marker of colorectal cancer by secretome analysis and quantitative proteomics. PMID: 25216327
  2. PC3 tumors are sustained by a small number of tumor-initiating cells with stem-like characteristics, including strong self-renewal and pro-angiogenic capability and marked by the expression pattern FAM65Bhigh/MFI2low/LEF1low. PMID: 21190562
  3. The human genome contains a second melanotransferrin gene (MTf2). There are two isoforms of melanotransferrin. PMID: 11852110
  4. its ability to donate 59Fe to SK-Mel-28 melanoma cells and other cell types PMID: 12230555
  5. membrane-bound and soluble p97 affect the migration capacity of endothelial and melanoma cells and suggest that p97 could be involved in the regulation of plasminogen activation by interacting with pro-uPA and plasminogen. PMID: 12750156
  6. Serum melanotransferrin exists in various conformations depending on the binding of bivalent cations or following post-translational modification; serum melanotransferrin levels are unchanged in subjects with Alzheimer's disease. PMID: 12809550
  7. The distribution of MTf and its splice variants may provide clues to their possible biological roles in melanoma and other tumors. PMID: 15716025
  8. melanotransferrin has a role in cell proliferation and tumorigenesis PMID: 16704991
  9. Anti-angiogenic properties of soluble melanotransferrin may result from local overstimulation of plasminogen activation by tissue plasminogen activator, thus leading to subsequent degradation of the fibronectin matrix and endothelial cell detachment. PMID: 16713448
  10. Altogether, these findings strongly suggest that MTf overexpression in melanoma cells contributes to tumor progression by stimulating plasmin generation as well as cell migration and invasion. PMID: 17196552
  11. HMB-45 (melanoma-associated antigen p97) and Melan-A combined were positive in 100% of the renal angiomyolipomas PMID: 17227112
  12. Novel molecular targets directly or indirectly regulated by MTf and the potential pathways involved in its function, including modulation of proliferation. PMID: 17449903
  13. this review discusses the studies that have demonstrated that melanotransferrin is not involved in iron metabolism, but plays a vital role in melanoma cell proliferation and tumorigenesis. PMID: 17452986
  14. Biochemical and spectroscopic studies of MTF1 are reported. PMID: 18691669

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Subcellular Location
[Isoform 1]: Cell membrane; Lipid-anchor, GPI-anchor.
Protein Families
Transferrin family
Tissue Specificity
Found predominantly in human melanomas and in certain fetal tissues; also found in liver, epithelium, umbilical chord, placenta and sweat gland ducts.
Database Links

HGNC: 7037

OMIM: 155750

KEGG: hsa:4241

STRING: 9606.ENSP00000296350

UniGene: Hs.184727

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