Product Details

Purity

Greater than 95% as determined by SDS-PAGE.
Greater than 95% as determined by SEC-HPLC.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Human MELTF at 2 μg/mL can bind Anti-MELTF recombinant antibody (CSB-RA013754MA1HU). The EC50 is 0.3476-0.4529 ng/mL.

Target Names

MELTF

Uniprot No.

P08582

Alternative Names

Melanotransferrin; Melanoma-associated antigen p97; CD228; MELTF; MAP97, MFI2

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

20-709aa

Target Protein Sequence

GMEVRWCATSDPEQHKCGNMSEAFREAGIQPSLLCVRGTSADHCVQLIAAQEADAITLDGGAIYEAGKEHGLKPVVGEVYDQEVGTSYYAVAVVRRSSHVTIDTLKGVKSCHTGINRTVGWNVPVGYLVESGRLSVMGCDVLKAVSDYFGGSCVPGAGETSYSESLCRLCRGDSSGEGVCDKSPLERYYDYSGAFRCLAEGAGDVAFVKHSTVLENTDGKTLPSWGQALLSQDFELLCRDGSRADVTEWRQCHLARVPAHAVVVRADTDGGLIFRLLNEGQRLFSHEGSSFQMFSSEAYGQKDLLFKDSTSELVPIATQTYEAWLGHEYLHAMKGLLCDPNRLPPYLRWCVLSTPEIQKCGDMAVAFRRQRLKPEIQCVSAKSPQHCMERIQAEQVDAVTLSGEDIYTAGKTYGLVPAAGEHYAPEDSSNSYYVVAVVRRDSSHAFTLDELRGKRSCHAGFGSPAGWDVPVGALIQRGFIRPKDCDVLTAVSEFFNASCVPVNNPKNYPSSLCALCVGDEQGRNKCVGNSQERYYGYRGAFRCLVENAGDVAFVRHTTVFDNTNGHNSEPWAAELRSEDYELLCPNGARAEVSQFAACNLAQIPPHAVMVRPDTNIFTVYGLLDKAQDLFGDDHNKNGFKMFDSSNYHGQDLLFKDATVRAVPVGEKTTYRGWLGLDYVAALEGMSSQQC

Mol. Weight

76.9 kDa

Protein Length

Full Length of Mature Protein of Isoform 1

Tag Info

C-terminal 10xHis-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Melanotransferrin functions as a membrane-bound iron-binding glycoprotein implicated in iron homeostasis and neural development, making it a valuable target for investigating metal transport mechanisms and melanoma biology. This full-length mature protein (aa 20–709) demonstrates robust antibody-binding activity with an EC50 of 0.3476–0.4529 ng/mL in functional ELISA, confirming that the recombinant construct retains the conformational epitopes required for antibody development, immunoassay standardization, and ligand-binding studies. The protein meets the quality thresholds commonly required for drug-protein interaction assays and membrane transporter functional studies, with purity exceeding 95% by SDS-PAGE and endotoxin levels below 1.0 EU/μg. Researchers examining iron sequestration pathways, characterizing MELTF-specific monoclonal antibodies, or performing structural analyses of transferrin family members will find this mammalian-expressed protein provides a suitable basis for experiments demanding native glycosylation and proper disulfide bond formation.

Target Background

Function

Involved in iron cellular uptake. Seems to be internalized and then recycled back to the cell membrane. Binds a single atom of iron per subunit. Could also bind zinc.

Gene References into Functions

Melanotransferrin is a serological marker of colorectal cancer by secretome analysis and quantitative proteomics. PMID: 25216327
PC3 tumors are sustained by a small number of tumor-initiating cells with stem-like characteristics, including strong self-renewal and pro-angiogenic capability and marked by the expression pattern FAM65Bhigh/MFI2low/LEF1low. PMID: 21190562
The human genome contains a second melanotransferrin gene (MTf2). There are two isoforms of melanotransferrin. PMID: 11852110
its ability to donate 59Fe to SK-Mel-28 melanoma cells and other cell types PMID: 12230555
membrane-bound and soluble p97 affect the migration capacity of endothelial and melanoma cells and suggest that p97 could be involved in the regulation of plasminogen activation by interacting with pro-uPA and plasminogen. PMID: 12750156
Serum melanotransferrin exists in various conformations depending on the binding of bivalent cations or following post-translational modification; serum melanotransferrin levels are unchanged in subjects with Alzheimer's disease. PMID: 12809550
The distribution of MTf and its splice variants may provide clues to their possible biological roles in melanoma and other tumors. PMID: 15716025
melanotransferrin has a role in cell proliferation and tumorigenesis PMID: 16704991
Anti-angiogenic properties of soluble melanotransferrin may result from local overstimulation of plasminogen activation by tissue plasminogen activator, thus leading to subsequent degradation of the fibronectin matrix and endothelial cell detachment. PMID: 16713448
Altogether, these findings strongly suggest that MTf overexpression in melanoma cells contributes to tumor progression by stimulating plasmin generation as well as cell migration and invasion. PMID: 17196552
HMB-45 (melanoma-associated antigen p97) and Melan-A combined were positive in 100% of the renal angiomyolipomas PMID: 17227112
Novel molecular targets directly or indirectly regulated by MTf and the potential pathways involved in its function, including modulation of proliferation. PMID: 17449903
this review discusses the studies that have demonstrated that melanotransferrin is not involved in iron metabolism, but plays a vital role in melanoma cell proliferation and tumorigenesis. PMID: 17452986
Biochemical and spectroscopic studies of MTF1 are reported. PMID: 18691669

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Subcellular Location

[Isoform 1]: Cell membrane; Lipid-anchor, GPI-anchor.

Protein Families

Transferrin family

Tissue Specificity

Found predominantly in human melanomas and in certain fetal tissues; also found in liver, epithelium, umbilical chord, placenta and sweat gland ducts.

Database Links

HGNC: 7037

OMIM: 155750

KEGG: hsa:4241

STRING: 9606.ENSP00000296350

UniGene: Hs.184727

Code	CSB-MP013754HU1
Abbreviation	Recombinant Human MELTF protein (Active)
MSDS	MSDS Datasheet
Size	$190
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized Human MELTF at 2 μg/ml can bind Anti-MELTF recombinant antibody (CSB-RA013754MA1HU). The EC₅₀ is 0.3476-0.4529 ng/mL. Biological Activity Assay The purity of MELTF was greater than 95% as determined by SEC-HPLC
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Recombinant Human Melanotransferrin (MELTF) (Active)

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