Recombinant Human Melanotransferrin (MFI2)

Code CSB-BP013754HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-EP013754HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-MP013754HU
MSDS
Size Pls inquire
Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MELTF
Uniprot No.
Alternative Names
Antigen p97; CD228; CD228 antigen; MAP97 ; Melanoma associated antigen p97; Melanoma-associated antigen p97; Melanotransferrin; Mfi2; MTF1 ; TRFM_HUMAN
Species
Homo sapiens (Human)
Expression Region
20-302
Target Protein Sequence
GMEVRWCATSDPEQHKCGNMSEAFREAGIQPSLLCVRGTSADHCVQLIAAQEADAITLDGGAIYEAGKEHGLKPVVGEVYDQEVGTSYYAVAVVRRSSHVTIDTLKGVKSCHTGINRTVGWNVPVGYLVESGRLSVMGCDVLKAVSDYFGGSCVPGAGETSYSESLCRLCRGDSSGEGVCDKSPLERYYDYSGAFRCLAEGAGDVAFVKHSTVLENTDESPSRRQTWTRSEEEEGECPAHEEARRTMRSSAGQAWKWAPVHRPQDESDKGEFGKRAKSRDMLG
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in iron cellular uptake. Seems to be internalized and then recycled back to the cell membrane. Binds a single atom of iron per subunit. Could also bind zinc.
Gene References into Functions
  1. Melanotransferrin is a serological marker of colorectal cancer by secretome analysis and quantitative proteomics. PMID: 25216327
  2. PC3 tumors are sustained by a small number of tumor-initiating cells with stem-like characteristics, including strong self-renewal and pro-angiogenic capability and marked by the expression pattern FAM65Bhigh/MFI2low/LEF1low. PMID: 21190562
  3. The human genome contains a second melanotransferrin gene (MTf2). There are two isoforms of melanotransferrin. PMID: 11852110
  4. its ability to donate 59Fe to SK-Mel-28 melanoma cells and other cell types PMID: 12230555
  5. membrane-bound and soluble p97 affect the migration capacity of endothelial and melanoma cells and suggest that p97 could be involved in the regulation of plasminogen activation by interacting with pro-uPA and plasminogen. PMID: 12750156
  6. Serum melanotransferrin exists in various conformations depending on the binding of bivalent cations or following post-translational modification; serum melanotransferrin levels are unchanged in subjects with Alzheimer's disease. PMID: 12809550
  7. The distribution of MTf and its splice variants may provide clues to their possible biological roles in melanoma and other tumors. PMID: 15716025
  8. melanotransferrin has a role in cell proliferation and tumorigenesis PMID: 16704991
  9. Anti-angiogenic properties of soluble melanotransferrin may result from local overstimulation of plasminogen activation by tissue plasminogen activator, thus leading to subsequent degradation of the fibronectin matrix and endothelial cell detachment. PMID: 16713448
  10. Altogether, these findings strongly suggest that MTf overexpression in melanoma cells contributes to tumor progression by stimulating plasmin generation as well as cell migration and invasion. PMID: 17196552
  11. HMB-45 (melanoma-associated antigen p97) and Melan-A combined were positive in 100% of the renal angiomyolipomas PMID: 17227112
  12. Novel molecular targets directly or indirectly regulated by MTf and the potential pathways involved in its function, including modulation of proliferation. PMID: 17449903
  13. this review discusses the studies that have demonstrated that melanotransferrin is not involved in iron metabolism, but plays a vital role in melanoma cell proliferation and tumorigenesis. PMID: 17452986
  14. Biochemical and spectroscopic studies of MTF1 are reported. PMID: 18691669

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Subcellular Location
[Isoform 1]: Cell membrane; Lipid-anchor, GPI-anchor.
Protein Families
Transferrin family
Tissue Specificity
Found predominantly in human melanomas and in certain fetal tissues; also found in liver, epithelium, umbilical chord, placenta and sweat gland ducts.
Database Links

HGNC: 7037

OMIM: 155750

KEGG: hsa:4241

STRING: 9606.ENSP00000296350

UniGene: Hs.184727

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