MELTF Antibody

Datasheet

Code	CSB-PA013754LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: HEK293 whole cell lysate, Hela whole cell lysate All lanes: MELTF antibody at 3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 81, 33 kDa Observed band size: 81 kDa Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA013754LA01HU at dilution of 1:100 Immunofluorescent analysis of HepG2 cells using CSB-PA013754LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) MELTF Polyclonal antibody

Uniprot No.

P08582

Target Names

MELTF

Alternative Names

Antigen p97 antibody; CD228 antibody; CD228 antigen antibody; MAP97 antibody; Melanoma associated antigen p97 antibody; Melanoma-associated antigen p97 antibody; Melanotransferrin antibody; Mfi2 antibody; MTF1 antibody; TRFM_HUMAN antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Melanotransferrin protein (20-302AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The MELTF Antibody (Product code: CSB-PA013754LA01HU) is Non-conjugated. For MELTF Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA013754LB01HU	MELTF Antibody, HRP conjugated	ELISA
FITC	CSB-PA013754LC01HU	MELTF Antibody, FITC conjugated
Biotin	CSB-PA013754LD01HU	MELTF Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The polyclonal antibody CSB-PA013754LA01HU especially targets the MELTF protein. To create this antibody, a rabbit was inoculated with recombinant human Melanotransferrin protein (20-302aa), and protein G affinity chromatography was employed to purify the antibody. The purity of this MELTF polyclonal antibody is greater than 95%.

The MELTF polyclonal antibody can detect MELTF protein in both human and mouse samples. Multiple rigorous validations have confirmed its efficacy in various applications, including ELISA, WB, IHC, and IF. Therefore, researchers can utilize this antibody to quantify MELTF protein levels, identify its presence and size, and localize the MELTF protein.

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Target Background

Function

Involved in iron cellular uptake. Seems to be internalized and then recycled back to the cell membrane. Binds a single atom of iron per subunit. Could also bind zinc.

Gene References into Functions

Melanotransferrin is a serological marker of colorectal cancer by secretome analysis and quantitative proteomics. PMID: 25216327
PC3 tumors are sustained by a small number of tumor-initiating cells with stem-like characteristics, including strong self-renewal and pro-angiogenic capability and marked by the expression pattern FAM65Bhigh/MFI2low/LEF1low. PMID: 21190562
The human genome contains a second melanotransferrin gene (MTf2). There are two isoforms of melanotransferrin. PMID: 11852110
its ability to donate 59Fe to SK-Mel-28 melanoma cells and other cell types PMID: 12230555
membrane-bound and soluble p97 affect the migration capacity of endothelial and melanoma cells and suggest that p97 could be involved in the regulation of plasminogen activation by interacting with pro-uPA and plasminogen. PMID: 12750156
Serum melanotransferrin exists in various conformations depending on the binding of bivalent cations or following post-translational modification; serum melanotransferrin levels are unchanged in subjects with Alzheimer's disease. PMID: 12809550
The distribution of MTf and its splice variants may provide clues to their possible biological roles in melanoma and other tumors. PMID: 15716025
melanotransferrin has a role in cell proliferation and tumorigenesis PMID: 16704991
Anti-angiogenic properties of soluble melanotransferrin may result from local overstimulation of plasminogen activation by tissue plasminogen activator, thus leading to subsequent degradation of the fibronectin matrix and endothelial cell detachment. PMID: 16713448
Altogether, these findings strongly suggest that MTf overexpression in melanoma cells contributes to tumor progression by stimulating plasmin generation as well as cell migration and invasion. PMID: 17196552
HMB-45 (melanoma-associated antigen p97) and Melan-A combined were positive in 100% of the renal angiomyolipomas PMID: 17227112
Novel molecular targets directly or indirectly regulated by MTf and the potential pathways involved in its function, including modulation of proliferation. PMID: 17449903
this review discusses the studies that have demonstrated that melanotransferrin is not involved in iron metabolism, but plays a vital role in melanoma cell proliferation and tumorigenesis. PMID: 17452986
Biochemical and spectroscopic studies of MTF1 are reported. PMID: 18691669

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Subcellular Location

[Isoform 1]: Cell membrane; Lipid-anchor, GPI-anchor.

Protein Families

Transferrin family

Tissue Specificity

Found predominantly in human melanomas and in certain fetal tissues; also found in liver, epithelium, umbilical chord, placenta and sweat gland ducts.

Database Links

HGNC: 7037

OMIM: 155750

KEGG: hsa:4241

STRING: 9606.ENSP00000296350

UniGene: Hs.184727

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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