Human Mitochondrial import inner membrane translocase subunit TIM14(DNAJC19) ELISA kit

Code CSB-EL007026HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
DnaJ (Hsp40) homolog, subfamily C, member 19
Alternative Names
DnaJ (Hsp40) homolog; subfamily C; member 19 ELISA Kit; DnaJ homolog subfamily C member 19 ELISA Kit; DNAJC19 ELISA Kit; homolog of yeast TIM14 ELISA Kit; Mitochondrial import inner membrane translocase subunit TIM14 ELISA Kit; PAM18 ELISA Kit; TIM 14 ELISA Kit; TIM14 ELISA Kit; TIM14_HUMAN ELISA Kit; TIMM 14 ELISA Kit; TIMM14 ELISA Kit; translocase of the inner mitochondrial membrane 14 ELISA Kit; Translocase of the inner mitochondrial membrane 14; yeast homolog of ELISA Kit
Abbreviation
DNAJC19
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
28 pg/mL-1800 pg/mL
Sensitivity
7 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human DNAJC19 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %97
Range %92-101
1:2Average %86
Range %81-90
1:4Average %93
Range %89-98
1:8Average %90
Range %85-94
Recovery
The recovery of human DNAJC19 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10399-107
EDTA plasma (n=4)9590-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
18002.656 2.719 2.688 2.579
9002.287 2.172 2.230 2.121
4501.653 1.707 1.680 1.571
2251.135 1.071 1.103 0.994
112.50.681 0.653 0.667 0.558
560.374 0.362 0.368 0.259
280.257 0.252 0.255 0.146
00.112 0.105 0.109
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human DNAJC19 ELISA Kit was designed for the quantitative measurement of Human DNAJC19 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 28 pg/mL-1800 pg/mL and the sensitivity is 7 pg/mL.

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Target Background

Function
(From Uniprot)
Mitochondrial co-chaperone which forms a complex with prohibitins to regulate cardiolipin remodeling. May be a component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. May act as a co-chaperone that stimulate the ATP-dependent activity.
Gene References into Functions
  1. Both DnaJC15 and DnaJC19 formed two distinct subcomplexes with Magmas at the import channel. PMID: 27330077
  2. The loss of DNAJC19/PHB complexes affects cardiolipin acylation and leads to the accumulation of cardiolipin species with altered acyl chains. PMID: 24856930
  3. A report of a new mutation in the human DNAJC19 gene that causes early onset dilated cardiomyopathy syndrome in two brothers of Finnish origin. PMID: 22797137
  4. The association of dilated cardiomyopathy with ataxia (DCMA) syndrome with a segment of 3q26.33 and the identification of a splice mutation in a novel gene DNAJC19 in DCMA patients. PMID: 16055927
  5. Dilated cardiomyopathy caused by homozygous mutations in a novel gene, DNAJC19, presumed to play a role in importation of mitochondrial proteins. PMID: 17244376

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Involvement in disease
3-methylglutaconic aciduria 5 (MGA5)
Subcellular Location
Mitochondrion inner membrane; Single-pass membrane protein; Matrix side.
Protein Families
TIM14 family
Tissue Specificity
Ubiquitously expressed.
Database Links

HGNC: 30528

OMIM: 608977

KEGG: hsa:131118

STRING: 9606.ENSP00000372005

UniGene: Hs.230601

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