Human Monocyte Chemotactic Protein 4(MCP-4/CCL13) ELISA Kit

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Human Monocyte Chemotactic Protein 4(MCP-4/CCL13) ELISA Kit

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Protocol

Code	CSB-E09762h-IS
Size	96T,5×96T,10×96T
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Product Details
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Target Background

Target Name

monocyte chemotactic protein 4

Associated names

C-C motif chemokine 13 ELISA Kit; CCL13 ELISA Kit; CCL13_HUMAN ELISA Kit; Chemokine (C C motif) ligand 13 ELISA Kit; CK beta 10 ELISA Kit; CK-beta-10 ELISA Kit; CKb10 ELISA Kit; MCP-4 ELISA Kit; MGC17134 ELISA Kit; Monocyte chemoattractant protein 4 ELISA Kit; Monocyte chemotactic protein 4 ELISA Kit; NCC 1 ELISA Kit; NCC-1 ELISA Kit; NCC1 ELISA Kit; New CC chemokine 1 ELISA Kit; SCYA13 ELISA Kit; SCYL1 ELISA Kit; short chain ELISA Kit; Small inducible cytokine A13 precursor ELISA Kit; Small inducible cytokine subfamily A (Cys Cys) member 13 ELISA Kit; Small-inducible cytokine A13 ELISA Kit

Abbreviation

CCL13

Uniprot No.

Q99616

Species

Homo sapiens (Human)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

3.12pg/ml-200pg/ml

Sensitivity

1.56pg/ml

Assay Time

3.5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human MCP-4/CCL13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:2	Average %	97
	Range %	93-103
1:4	Average %	107
	Range %	98-112
1:8	Average %	87
	Range %	82-95
1:16	Average %	113
	Range %	106-118

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	3.095	2.935	3.015	2.950
100	2.085	2.142	2.113	2.048
50	1.135	1.061	1.098	1.032
25	0.643	0.612	0.627	0.562
12.5	0.359	0.367	0.363	0.297
6.25	0.205	0.195	0.200	0.135
3.12	0.144	0.138	0.141	0.076
0	0.066	0.065	0.065

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function
(From Uniprot)

Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. Signals through CCR2B and CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic and non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens.

Gene References into Functions

CCL13 expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
CCL13 levels in serum and synovial fluid may serve as a biomarker for the progression of osteoarthritis. PMID: 25654294
CCL13 is an antimicrobial protein with bacteriocidal activity against E. coli. PMID: 12949249
Data suggest that CCL13 binds to several chemokine receptors (CCR1, CCR2, and CCR3), allowing CCL13 to elicit different effects on target cells of immune system. CCL13 is involved in pathology of chronic inflammatory/autoimmune diseases. [REVIEW] PMID: 23846739
MCP-4 and hsCRP may be the markers linking chronic inflammation in obesity and periodontal disease. PMID: 23375121
E(2) has adverse effects on the pathogenesis of RA as a result of unregulated cell death, increased TNF-alpha-induced MMP-3 production, and CCL13 overproduction, subsequently resulting in the disease progression of RA. PMID: 22393877
Findings suggest that the pro-atherogenic effects of CCR2 may not be restricted to interaction with MCP-1, but could also involve activation by MCP-4, being an inflammatory link between platelet and monocyte activation. PMID: 20139115
Data suggest that the monocyte chemoattractant protein-4 core promoter YY-1 binding motif is functional, and modulates the transcriptional regulation of the MCP-4 gene. PMID: 12805085
The study was undertaken to understand the relationship between the functional proteomics of receptor-Ck and developmental stages of human atherosclerotic aortic wall. PMID: 14560935
MCP-4 may be a critical molecule that links obesity and chronic inflammation. PMID: 16855189
The Th2 cytokine IL-4 preferentially stimulated MCP-4 expression. PMID: 17541284
interferon-gamma with IL-1beta/TNF-alpha activates the production of CCL13 from chondrocytes in RA joints; secreted CCL13 enhances fibroblast-like synoviocyte proliferation by activating the extracellular signal-regulated MAP kinases cascade PMID: 17824960
TLR2, 3, 4 and 5 ligands, but not TLR7/8 or 9 ligands, synergized with IL-4 to induce the production of MCP-4 in nasal polyp fibroblasts. PMID: 17851891
CCL13 and HSPC159 mRNA expressions in PBMC are up-regulated specifically in MCNS patients during the nephrosis phase. PMID: 18219197
Oncostatin M can strongly up-regulate the expression of CCL13, a chemokine recently identified in the synovial fluid of patients with rheumatoid arthritis. PMID: 19565514

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Subcellular Location

Secreted.

Protein Families

Intercrine beta (chemokine CC) family

Tissue Specificity

Widely expressed. Found in small intestine, thymus, colon, lung, trachea, stomach and lymph node. Low levels seen in the pulmonary artery smooth muscle cells.

Database Links

HGNC: 10611

OMIM: 601391

KEGG: hsa:6357

STRING: 9606.ENSP00000225844

UniGene: Hs.414629

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Related pathways

Chemokine signaling pathway NF-kappa B signaling pathway

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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