Human Osteopontin,OPN ELISA Kit

Product Details

Citations

Related Products

Customer Reviews and Q&A

Target Background

Product Details

Target Name

secreted phosphoprotein 1

Alternative Names

BNSP ELISA Kit; Bone sialoprotein 1 ELISA Kit; Bone sialoprotein I ELISA Kit; BSP I ELISA Kit; BSPI ELISA Kit; Early T lymphocyte activation 1 ELISA Kit; ETA 1 ELISA Kit; ETA1 ELISA Kit; MGC110940 ELISA Kit; Nephropontin ELISA Kit; OPN ELISA Kit; Osteopontin ELISA Kit; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein ELISA Kit; OSTP_HUMAN ELISA Kit; PSEC0156 ELISA Kit; secreted phosphoprotein 1 (osteopontin bone sialoprotein I early T lymphocyte activation 1) ELISA Kit; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1) ELISA Kit; Secreted phosphoprotein 1 ELISA Kit; SPP 1 ELISA Kit; SPP-1 ELISA Kit; SPP1 ELISA Kit; SPP1/CALPHA1 fusion ELISA Kit; Urinary stone protein ELISA Kit; Uropontin ELISA Kit

Abbreviation

OPN

Uniprot No.

P10451

Species

Homo sapiens (Human)

Sample Types

serum, plasma, urine, tissue homogenates

Detection Range

0.78 ng/mL-50 ng/mL

Sensitivity

0.195 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cancer

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human OPN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	94
	Range %	88-98
1:10	Average %	100
	Range %	94-104
1:20	Average %	93
	Range %	88-97
1:40	Average %	99
	Range %	93-104

Recovery

The recovery of human OPN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	81-97
EDTA plasma (n=4)	102	97-106

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
50	2.418	2.333	2.376	2.271
25	1.684	1.706	1.695	1.590
12.5	1.175	1.152	1.164	1.059
6.25	0.654	0.664	0.659	0.554
3.12	0.383	0.361	0.372	0.267
1.56	0.245	0.232	0.239	0.134
0.78	0.176	0.185	0.181	0.076
0	0.102	0.108	0.105

Materials provided

A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human OPN antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled OPN antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
An instruction manual

Materials not provided

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
50-300ul multi-channel micropipette
Pipette tips
Single-channel micropipette with different ranges
100ml and 500ml graduated cylinders
Deionized or distilled water
Timer
Test tubes for dilution

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

The Human Osteopontin (OPN) ELISA Kit is a powerful tool for researchers in the field of cancer. This quantitative assay measures levels of OPN, a protein involved in the regulation of bone remodeling and implicated in various diseases including cancer（tumor），bone metabolism, immunity, growth and development.

With a detection range of 0.78 ng/mL-50 ng/mL and a sensitivity of 0.195 ng/mL, this ELISA kit can detect low levels of OPN with high accuracy. It can be used with a variety of sample types, including serum, plasma, urine, and tissue homogenates, making it a versatile tool for research.

The assay principle is based on the sandwich method, where a monoclonal antibody specific to human OPN is pre-coated onto a microplate. The sample is added, and any OPN present in the sample binds to the antibody. A biotinylated polyclonal antibody specific to human OPN is then added, which binds to the OPN-antibody complex. Finally, streptavidin conjugated to horseradish peroxidase (HRP) is added, which binds to the biotinylated antibody. The HRP produces a colorimetric reaction in the presence of a substrate, which can be measured at a wavelength of 450 nm.

The assay can be completed in as little as 1-5 hours and requires a sample volume of only 50-100ul, minimizing the amount of sample required for analysis. The kit includes all necessary components and can be used with standard laboratory equipment.

With 12 citations, this kit has been validated by the scientific community and is a trusted tool for cancer research. So, whether you are studying the role of OPN in cancer progression or investigating potential therapeutic targets, this Human Osteopontin (OPN) ELISA Kit is an essential addition to your research arsenal.

Code	CSB-E08392h
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase in six months till depleted. Apply now

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.