Product Details

Purity

≥ 95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA.Immobilized Human SPP1 at 2 μg/mL can bind Anti-SPP1 recombinant antibody(CSB-RA022603MA1HU).The EC50 is 4.929-5.425 ng/mL.

Target Names

SPP1

Uniprot No.

P10451

Alternative Names

Osteopontin; Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Uropontin

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

17-314aa

Target Protein Sequence

IPVKQADSGSSEEKQLYNKYPDAVATWLNPDPSQKQNLLAPQNAVSSEETNDFKQETLPSKSNESHDHMDDMDDEDDDDHVDSQDSIDSNDSDDVDDTDDSHQSDESHHSDESDELVTDFPTDLPATEVFTPVVPTVDTYDGRGDSVVYGLRSKSKKFRRPDIQYPDATDEDITSHMESEELNGAYKAIPVAQDLNAPSDWDSRGKDSYETSQLDDQSAETHSHKQSRLYKRKANDESNEHSDVIDSQELSKVSREFHSHEFHSHEDMLVVDPKSKEEDKHLKFRISHELDSASSEVN

Mol. Weight

35.1 kDa

Protein Length

Full Length of Mature Protein

Tag Info

C-terminal 10xHis-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Osteopontin mediates cell adhesion, migration, and survival across tumor microenvironments and inflammatory contexts, making quantitative assessment of its binding interactions essential for mechanistic studies. This full-length mature protein (aa 17–314) demonstrates specific antibody recognition with an EC50 of 4.929–5.425 ng/mL in functional ELISA, providing a validated reagent for antibody development workflows including epitope mapping, hybridoma screening, and immunoassay standardization. Mammalian expression preserves the native post-translational modifications critical to osteopontin's integrin-binding and CD44-binding functions, supporting cell-based adhesion assays, migration studies with cancer cell lines, and signaling pathway investigations of FAK, PI3K/AKT, and NF-κB activation in tumor biology models. The combination of >95% purity and <1.0 EU/μg endotoxin satisfies the quality thresholds commonly required for immune cell co-culture experiments where LPS contamination would confound osteopontin-driven macrophage polarization or T-cell activation readouts.

Code	CSB-MP022603HU
Abbreviation	Recombinant Human SPP1 protein (Active)
MSDS	MSDS Datasheet
Size	$85
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA.Immobilized Human SPP1 at 2 μg/ml can bind Anti-SPP1 recombinant antibody(CSB-RA022603MA1HU).The EC₅₀ is 4.929-5.425 ng/mL. Biological Activity Assay
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Recombinant Human Osteopontin (SPP1) (Active)

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