Code | CSB-RA261140A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
The production process for the SPP1 recombinant monoclonal antibody consists of four key steps: first, sequencing the SPP1 monoclonal antibody gene; second, cloning the gene into a plasmid vector; third, transfecting the recombinant vector into a host cell line; fourth, purifying the SPP1 recombinant monoclonal antibody from the cell culture supernatant using affinity chromatography and finally testing and characterizing the purified antibody. The SPP1 monoclonal antibody is produced using a synthesized peptide derived from human SPP1 as the immunogen. This resulting SPP1 recombinant monoclonal antibody is highly recommended for use in ELISA, WB, and IHC applications to detect human SPP1 protein.
SPP1, also known as osteopontin, plays a critical role in the regulation of various physiological and pathological processes in cells, including bone remodeling, immune response, cell migration, and cancer progression. SPP1 is a major component of the extracellular matrix of bone, and plays a critical role in the regulation of bone remodeling by mediating the adhesion and migration of osteoclasts and osteoblasts. SPP1 is involved in the regulation of the immune response by interacting with various immune cells, including macrophages, T cells, and natural killer cells, and modulating their function and activity. SPP1 is overexpressed in various types of cancer and has been implicated in the regulation of tumor progression and metastasis by promoting cancer cell proliferation, survival, invasion, and angiogenesis.
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