Human Placental alkaline phosphatase,PLAP ELISA Kit

Instructions
Code CSB-E09160h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name alkaline phosphatase, placental (Regan isozyme)
Alternative Names Alkaline phosphatase ELISA Kit; Alkaline phosphatase placental ELISA Kit; Alkaline phosphatase placental type ELISA Kit; Alkaline phosphatase Regan isozyme ELISA Kit; ALP ELISA Kit; Alp1 ELISA Kit; ALPP ELISA Kit; FLJ61142 ELISA Kit; Germ-cell alkaline phosphatase ELISA Kit; nagao isozyme ELISA Kit; OTTHUMP00000164354 ELISA Kit; PALP ELISA Kit; Placental alkaline phosphatase 1 ELISA Kit; placental heat-stable alkaline phosphatase ELISA Kit; placental type ELISA Kit; PLAP ELISA Kit; PLAP-1 ELISA Kit; PLAP1 ELISA Kit; PPB1_HUMAN ELISA Kit
Abbreviation ALPP
Uniprot No. P05187
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 84 pg/mL-2000 pg/mL
Sensitivity 34 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Tags & Cell Markers
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<10%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human PLAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 92  
Range % 85-98  
1:2 Average % 96  
Range % 88-100  
1:4 Average % 95  
Range % 89-100  
1:8 Average % 97  
Range % 91-101  
Recovery
The recovery of human PLAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 98 93-104  
EDTA plasma (n=4) 95 91-101  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2000 2.012 1.983 1.998 1.952  
1000 1.338 1.341 1.340 1.294  
500 0.775 0.828 0.802 0.756  
167 0.357 0.349 0.353 0.307  
84 0.224 0.235 0.230 0.184  
0 0.047 0.045 0.046    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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 Q&A
Q:

I would like to know whether the CSB-E09160h - Cusabio ELISA kit (quotation as attached) is able to quantify recombinant proteins (non-native)?

A:
Thanks for your inquiry. As in recombinant protein ,there is difference on expression system ,expression region and posttranslational modification,ect,we are not sure if recombinant proteins can be detected out.If the conformation of your recombinant proteins is same with native proteins ,then our kit can quantify it.

Target Data

Gene References into Functions
  1. This meta-analysis suggests that high serum ALP level is obviously associated with lower OS rate in patients with osteosarcoma, and it is an effective biomarker of prognosis. PMID: 29970708
  2. Concentrations of PLAP were elevated in gingival crevicular fluid of patients with pre-eclampsia. PMID: 26988336
  3. SALL4 also outperformed PLAP on a small sample of cytology blocks. Although SALL4 is not entirely specific, it is a highly sensitive marker with strong diffuse nuclear reactivity in the majority of MGCTs in the posttreatment setting, at significantly higher levels than PLAP PMID: 25906119
  4. Quantum-mechanical computational methods were employed to study the catalytic mechanism of human placental AP (PLAP). An active-site model was used, constructed on the basis of the X-ray crystal structure of the enzyme. PMID: 25409280
  5. term placental explants, but not their conditioned medium, can de-phosphorylate IGFBP-1 through the action of placental alkaline phosphatase PMID: 24856042
  6. Anti-PLAP antibodies may serve as a modular building blocks for the development of targeted therapeutic products, armed with cytotoxic drugs, radionuclides or cytokines as payloads. PMID: 24247025
  7. A candidate gene, ALPP, encoding the placental alkaline phosphatase, was identified as being potentially involved in recurrent spontaneous abortion. PMID: 24296104
  8. The objective of the following study was to record the specificity and sensitivity of alpha5(IV) loss, smoothelin expression and PLAP expression as markers of gastrointestinal smooth muscle neoplasms PMID: 24043717
  9. Data indicate that p180 is required for the efficient targeting of placental alkaline phosphatase (ALPP) mRNA to the endoplasmic reticulum (ER). PMID: 24019514
  10. Calculation of the electrostatic potentials within the active site of human placental alkaline phosphatase also suggests that the local positive electrostatic environment may account for its capability to distinguish various substrates PMID: 21910833
  11. High serum alkaline phosphatase cooperating with MMP-9 is associated with metastasis in patients with primary osteosarcoma. PMID: 22333159
  12. PLAP exerts a positive effect on DNA replication and acts as a proliferative factor in trophoblastic cells. PMID: 21868091
  13. The proximity of undifferentiated gonadal tissue with the tumors as well as the immunostaining patterns (PLAP+, OCT3/4+, and CD117/KIT+) suggests that germ cells found in them are a risk factor for gonadal tumors. PMID: 21692598
  14. the catalytic mechanism of human placental alkaline phosphatase PMID: 21939286
  15. Data show that serum alkaline phosphatase, Gleason score, and intensity of bone metastasis are important and statistically significant prognostic factors, and affects time to progression and life time. PMID: 19450995
  16. at a certain time point during adrenocortical development, some fetal zone cells survive owing to defective apoptosis and develop into childhood ACT, maintaining some characteristics of the embryonal period, such as PLAP expression PMID: 21516013
  17. Serum total calcium (r -0.1362, p<0.001), serum inorganic phosphate (r -0.45, p<0.001) and serum alkaline phosphatase (r -0.5587, p<0.001) have shown inverse relationship with age. PMID: 20655896
  18. High serum alkaline phosphatase is associated with chronic kidney disease. PMID: 20299338
  19. differential expression of Pl(1) and Pl(2) probably results from linkage disequilibrium with the sequence variation rs2014683G>A in the ALPP gene promoter that was shown to have allele-specific binding patterns to placental nuclear proteins. PMID: 20663553
  20. structure of placental alkaline phosphatase with pNPP contained only p-nitrophenol in three distinct sites, while the structure with 5'-AMP contained the p-nitrophenyl group in two of the sites instead of 5'-AMP PMID: 20693656
  21. Low magnitudes of tensile strain enhance expression of alkaline phosphatase in human osteoblasts. PMID: 19595020
  22. The PLAP D allele contains two amino acid substitutions: P209R (692C>G) and E429G (1352 A>G). PMID: 11857742
  23. Proximity of the protein moiety of a GPI-anchored protein to the membrane surface: a FRET study. PMID: 12081485
  24. the beta-N-acetylglucosaminyl phosphate diester residue is attached to the glycosylphosphatidylinositol anchor of human placental alkaline phosphatase and is a target of the channel-forming toxin aerolysin PMID: 12851398
  25. receptor for Aeromonas sobria hemolysin. PMID: 15715171
  26. analysis of human placental alkaline phosphatase in complex with functional ligands PMID: 15946677
  27. The effect of parity on placental weight and birth weight is examined through a series of birth records from an Indian population in Calcutta. PMID: 16431676
  28. crystal structure of strontium-substituted human placental alkaline phosphatase shows that strontium substitutes the calcium ion with concomitant modification of the metal coordination PMID: 16815919
  29. activity of GPI-anchored enzymes may be modulated by membrane microenvironment features PMID: 18416535
  30. Serum bilirubin, alkaline phosphatase, and aspartate aminotransferase are an efficient set of biochemistries to identify UDCA-treated patients with primary biliary cirrhosis at risk of death or liver transplantation (LT). PMID: 18752324
  31. Elevations of alkaline phosphatase is associated with therapy related pediatric cancer. PMID: 18802949

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Subcellular Location Cell membrane, Lipid-anchor, GPI-anchor
Protein Families Alkaline phosphatase family
Tissue Specificity Detected in placenta (at protein level).
Database Links

HGNC: 439

OMIM: 171800

KEGG: hsa:250

STRING: 9606.ENSP00000375881

UniGene: Hs.284255

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