Human Ras-related protein Rab-10(RAB10) ELISA kit

Code CSB-EL019152HU
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P61026
Lead Time 5-7 working days
Abbreviation RAB10
Protein Biological Process 1 Transport
Target Name RAB10, member RAS oncogene family
Alias , GTP-binding protein RAB10|ras-related GTP-binding protein|ras-related GTP-binding protein RAB10
Species Homo sapiens (Human)
Protein Biological Process 3 Protein transport
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 39 pg/mL-2500 pg/mL
Sensitivity 9.77 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
HGNC 9759
RGD 620879
MGI 105066
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human RAB10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 94
Range % 89-99
1:2 Average % 95
Range % 90-99
1:4 Average % 92
Range % 87-98
1:8 Average % 97
Range % 92-103
Recovery
The recovery of human RAB10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 101 98-105
EDTA plasma (n=4) 92 86-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2500 2.186 2.230 2.208 2.041
1250 1.724 1.619 1.672 1.505
625 1.223 1.165 1.194 1.027
312.5 0.744 0.753 0.749 0.582
156.25 0.516 0.503 0.510 0.343
78 0.368 0.378 0.373 0.206
39 0.259 0.250 0.255 0.088
0 0.166 0.168 0.167  
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Function The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (By similarity). That Rab is mainly involved in the biosynthetic transport of proteins from the Golgi to the plasma membrane. Regulates, for instance, SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the plasma membrane. In parallel, it regulates the transport of TLR4, a toll-like receptor to the plasma membrane and therefore may be important for innate immune response. Plays also a specific role in asymmetric protein transport to the plasma membrane within the polarized neuron and epithelial cells. In neurons, it is involved in axonogenesis through regulation of vesicular membrane trafficking toward the axonal plasma membrane while in epithelial cells, it regulates transport from the Golgi to the basolateral membrane. Moreover, may play a role in the basolateral recycling pathway and in phagosome maturation. According to PubMed
Subcellular Location Cytoplasmic vesicle membrane, Lipid-anchor, Cytoplasmic side, Golgi apparatus membrane, Golgi apparatus, trans-Golgi network membrane, Endosome membrane, Recycling endosome membrane, Cytoplasmic vesicle, phagosome membrane, Cell projection, cilium, Endoplasmic reticulum membrane
Protein Families Small GTPase superfamily, Rab family
Database Links

HGNC: 9759

OMIM: 612672

KEGG: hsa:10890

STRING: 9606.ENSP00000264710

UniGene: Hs.467960

Pathway AMPK signaling
Endocytosis

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