Purity
Greater than 90% as determined by SDS-PAGE.
Research Area
Signal Transduction
Alternative Names
GTP binding protein RAB10; Rab10; RAB10 member RAS oncogene family; RAB10_HUMAN; Ras related GTP binding protein; Ras related GTP binding protein RAB10; Ras-related protein Rab-10
Species
Homo sapiens (Human)
Expression Region
1-196aa
Target Protein Sequence
MAKKTYDLLFKLLLIGDSGVGKTCVLFRFSDDAFNTTFISTIGIDFKIKTVELQGKKIKLQIWDTAGQERFHTITTSYYRGAMGIMLVYDITNGKSFENISKWLRNIDEHANEDVERMLLGNKCDMDDKRVVPKGKGEQIAREHGIRFFETSAKANINIEKAFLTLAEDILRKTPVKEPNSENVDISSGGGVTGWK
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The expression region of this recombinant Human RAB10 covers amino acids 1-196. This RAB10 protein is expected to have a theoretical molecular weight of 26.1 kDa. This protein is generated in a e.coli-based system. The RAB10 coding gene included the N-terminal 6xHis tag, which simplifies the detection and purification processes of the recombinant RAB10 protein in following stages of expression and purification.
Ras-related protein Rab-10 (RAB10) is a member of the Rab GTPase family, which plays a crucial role in regulating intracellular membrane trafficking and vesicle transport. RAB10 specifically participates in the control of various cellular processes, including endocytosis, exocytosis, and organelle organization. It is primarily associated with the regulation of protein trafficking between the trans-Golgi network and recycling endosomes. The main function of RAB10 involves the coordination of vesicle transport and membrane fusion, contributing to the maintenance of cellular architecture and organelle dynamics. Research areas related to RAB10 often focus on understanding its role in cellular processes, including its involvement in the regulation of insulin-stimulated glucose transporter (GLUT4) trafficking, which has implications for metabolic disorders such as diabetes.