Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

RAB10

Uniprot No.

P61026

Research Area

Signal Transduction

Alternative Names

GTP binding protein RAB10; Rab10; RAB10 member RAS oncogene family; RAB10_HUMAN; Ras related GTP binding protein; Ras related GTP binding protein RAB10; Ras-related protein Rab-10

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-196aa

Target Protein Sequence

MAKKTYDLLFKLLLIGDSGVGKTCVLFRFSDDAFNTTFISTIGIDFKIKTVELQGKKIKLQIWDTAGQERFHTITTSYYRGAMGIMLVYDITNGKSFENISKWLRNIDEHANEDVERMLLGNKCDMDDKRVVPKGKGEQIAREHGIRFFETSAKANINIEKAFLTLAEDILRKTPVKEPNSENVDISSGGGVTGWK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

26.1kDa

Protein Length

Partial

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The expression region of this recombinant Human RAB10 covers amino acids 1-196. This RAB10 protein is expected to have a theoretical molecular weight of 26.1 kDa. This protein is generated in a e.coli-based system. The RAB10 coding gene included the N-terminal 6xHis tag, which simplifies the detection and purification processes of the recombinant RAB10 protein in following stages of expression and purification.

Ras-related protein Rab-10 (RAB10) is a member of the Rab GTPase family, which plays a crucial role in regulating intracellular membrane trafficking and vesicle transport. RAB10 specifically participates in the control of various cellular processes, including endocytosis, exocytosis, and organelle organization. It is primarily associated with the regulation of protein trafficking between the trans-Golgi network and recycling endosomes. The main function of RAB10 involves the coordination of vesicle transport and membrane fusion, contributing to the maintenance of cellular architecture and organelle dynamics. Research areas related to RAB10 often focus on understanding its role in cellular processes, including its involvement in the regulation of insulin-stimulated glucose transporter (GLUT4) trafficking, which has implications for metabolic disorders such as diabetes.

Target Background

Function

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is mainly involved in the biosynthetic transport of proteins from the Golgi to the plasma membrane. Regulates, for instance, SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the plasma membrane. In parallel, it regulates the transport of TLR4, a toll-like receptor to the plasma membrane and therefore may be important for innate immune response. Plays also a specific role in asymmetric protein transport to the plasma membrane. In neurons, it is involved in axonogenesis through regulation of vesicular membrane trafficking toward the axonal plasma membrane. In epithelial cells, it regulates transport from the Golgi to the basolateral membrane. May play a role in the basolateral recycling pathway and in phagosome maturation. May play a role in endoplasmic reticulum dynamics and morphology controlling tubulation along microtubules and tubules fusion. Together with LRRK2, RAB8A, and RILPL1, it regulates ciliogenesis. When phosphorylated by LRRK2 on Thr-73, binds RILPL1 and inhibits ciliogenesis.; (Microbial infection) Upon Legionella pneumophila infection promotes endoplasmic reticulum recruitment and bacterial replication. Plays a role in remodeling the Legionella-containing vacuole (LCV) into an endoplasmic reticulum-like vacuole.

Gene References into Functions

miR378a3p may act as a tumorsuppressor in Esophageal squamous cell carcinoma cells through negatively regulating Rab10. PMID: 29693138
Interrogating Parkinson's disease LRRK2 kinase pathway activity by assessing Rab10 phosphorylation in human neutrophils has been reported. PMID: 29127255
Our results suggest that RAB10 could be a promising therapeutic target for Alzheimer's Disease prevention PMID: 29183403
RAB10 regulates cell survival and proliferation through multiple oncogenic, cell stress and apoptosis pathways. More importantly, high RAB10 expression levels in HCC cells correlated with a poor prognosis in HCC patients. PMID: 28460436
miR-329 also suppresses wound-healing and migration ability of osteosarcoma cells and inhibits tumorigenicity in vivo. Rab10 was identified as a target of miR-329 in osteosarcoma and mediates its biofunction PMID: 27487475
Data suggest that MGCRABGAP is involved in mammalian spermiogenesis by modulating RAB10. PMID: 28067790
the target gene RAB10 regulated by miRNA-369-3p, miRNA-30e-5p, miRNA-30e-3p, and miRNA-655 may play key role in the progression and development of AD. PMID: 26082458
The Anaplasma phagocytophilum surface protein, uridine monophosphate kinase, was identified as a guanine nucleotide-independent, Rab10-specific ligand. PMID: 26289115
Rab10 is a target of the GTPase-activating protein AS160, which is inhibited after phosphorylation by the protein kinase Akt. PMID: 25103239
Rab10 silencing increased the plasma membrane residence of HAS3, resulting in a significant increase of hyaluronan secretion and an enlarged cell surface HA coat, whereas Rab10 overexpression suppressed hyaluronan synthesis. PMID: 24509846
Rab10 regulates endoplasmic reticulum dynamics/morphology. Suggest that these dynamics could be coupled to phospholipid synthesis. PMID: 23263280
Insulin-stimulated GLUT4 protein translocation in adipocytes requires the Rab10 guanine nucleotide exchange factor Dennd4C PMID: 21454697
Rab10 and Rab8A are new cytoplasmic factors implicated in WPB biogenesis that play a role in generating granules that can rapidly respond to secretagogue PMID: 21070595
Rab10, acting upstream of Rab5, plays a prominent role in phagolysosome formation and can modulate Mycobacterium-containing phagosomes maturation PMID: 20028485
Fibronectin 1 and RAB10 show elevated expression in uninvolved psoriatic epidermis. PMID: 15855153
a related Rab protein, Rab10, can interact with myosin Va, myosin Vb, and myosin Vc PMID: 19008234

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Subcellular Location

Cytoplasmic vesicle membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane. Golgi apparatus, trans-Golgi network membrane. Endosome membrane. Recycling endosome membrane. Cytoplasmic vesicle, phagosome membrane. Cytoplasm, cytoskeleton, cilium basal body. Endoplasmic reticulum membrane. Cytoplasm, perinuclear region.

Protein Families

Small GTPase superfamily, Rab family

Tissue Specificity

Expressed in the hippocampus. Expressed in neutrophils (at protein level).

Database Links

HGNC: 9759

OMIM: 612672

KEGG: hsa:10890

STRING: 9606.ENSP00000264710

UniGene: Hs.467960

Code	CSB-EP019152HU
Abbreviation	Recombinant Human RAB10 protein, partial
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Ras-related protein Rab-10 (RAB10), partial

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