Target Name
v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)
Alternative Names
c erbB 3 ELISA Kit; c erbB3 ELISA Kit; Erb b2 receptor tyrosine kinase 3 ELISA Kit; ErbB 3 ELISA Kit; ERBB3 ELISA Kit; ERBB3 protein ELISA Kit; erbB3 S ELISA Kit; ERBB3_HUMAN ELISA Kit; Glial growth factor receptor ELISA Kit; HER 3 ELISA Kit; HER3 ELISA Kit; Human epidermal growth factor receptor 3 ELISA Kit; LCCS2 ELISA Kit; MDA BF 1 ELISA Kit; MGC88033 ELISA Kit; p180 ErbB3 ELISA Kit; p45 sErbB3 ELISA Kit; p85 sErbB3 ELISA Kit; proto-oncogene-like protein c ErbB 3 ELISA Kit; proto-oncogene-like protein c ErbB3 ELISA Kit; Proto-oncogene-like protein c-ErbB-3 ELISA Kit; Receptor tyrosine protein kinase erbB 3 ELISA Kit; Receptor tyrosine protein kinase erbB3 ELISA Kit; Receptor tyrosine-protein kinase erbB-3 ELISA Kit; Tyrosine kinase type cell surface receptor HER3 ELISA Kit; Tyrosine kinase-type cell surface receptor HER3 ELISA Kit; v erb b2 avian erythroblastic leukemia viral oncogene homolog 3 ELISA Kit; v erb b2 erythroblastic leukemia viral oncogene homolog 3 (avian) ELISA Kit; v erb b2 erythroblastic leukemia viral oncogene homolog 3 ELISA Kit
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
125 pg/mL-8000 pg/mL
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Precision
Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
Three samples of known concentration were tested twenty times on one plate to assess. | | |
Inter-assay Precision (Precision between assays): CV%<10% | | | | |
Three samples of known concentration were tested in twenty assays to assess. | | | |
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Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ERBB3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| Sample | Serum(n=4) | | |
1:1 | Average % | 87 | | |
Range % | 82-94 | | |
1:2 | Average % | 98 | | |
Range % | 94-103 | | |
1:4 | Average % | 102 | | |
Range % | 98-106 | | |
1:8 | Average % | 95 | | |
Range % | 90-100 | | |
Recovery
The recovery of human ERBB3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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Sample Type | Average % Recovery | Range | | |
Serum (n=5) | 92 | 88-97 | | |
EDTA plasma (n=4) | 103 | 96-110 | | |
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Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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pg/ml | OD1 | OD2 | Average | Corrected | | | 8000 | 2.292 | 2.267 | 2.280 | 2.172 | | | 4000 | 1.472 | 1.398 | 1.435 | 1.327 | | | 2000 | 0.956 | 0.998 | 0.977 | 0.869 | | | 1000 | 0.635 | 0.665 | 0.650 | 0.542 | | | 500 | 0.364 | 0.352 | 0.358 | 0.250 | | | 250 | 0.225 | 0.212 | 0.219 | 0.111 | | | 125 | 0.171 | 0.181 | 0.176 | 0.068 | | | 0 | 0.107 | 0.109 | 0.108 | | | |
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Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx