Alternative Names
Anionic trypsinogen ELISA Kit; EC 3.4.21.4 ELISA Kit; MGC111183 ELISA Kit; MGC120174 ELISA Kit; Protease serine 2 preproprotein ELISA Kit; Protease; serine; 2 (trypsin 2) ELISA Kit; Protease; serine; 2 ELISA Kit; Protease; serine; 2; preproprotein ELISA Kit; Prss2 ELISA Kit; Serine protease 2 ELISA Kit; TRY2 ELISA Kit; TRY2_HUMAN ELISA Kit; TRY8 ELISA Kit; TRYP2 ELISA Kit; Trypsin 2 ELISA Kit; Trypsin II ELISA Kit; Trypsin-2 ELISA Kit; Trypsinogen 2 ELISA Kit
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.78 ng/ml-50 ng/ml.
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Precision
Intra-assay Precision (Precision within an assay): CV%<8% | | | |
Three samples of known concentration were tested twenty times on one plate to assess. | |
Inter-assay Precision (Precision between assays): CV%<10% | | | |
Three samples of known concentration were tested in twenty assays to assess. | | |
| Intra-Assay Precision | Inter-Assay Precision | Sample | 1 | 2 | 3 | 1 | 2 | 3 | n | 20 | 20 | 20 | 20 | 20 | 20 | Mean(ng/ml) | 5.828 | 6.052 | 6.250 | 5.828 | 5.970 | 6.314 | SD | 0.034 | 0.039 | 0.045 | 0.038 | 0.049 | 0.055 | CV(%) | 4.043 | 4.519 | 5.102 | 4.518 | 5.731 | 6.194 |
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Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human Trypsin-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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| Sample | Serum(n=4) |
1:1 | Average % | 92 |
Range % | 87-98 |
1:2 | Average % | 98 |
Range % | 93-104 |
1:4 | Average % | 84 |
Range % | 82-88 |
1:8 | Average % | 90 |
Range % | 85-97 |
Recovery
The recovery of human Trypsin-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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Sample Type | Average % Recovery | Range |
Serum (n=5) | 95 | 89-99 |
EDTA plasma (n=4) | 96 | 90-102 |
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Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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ng/ml | OD1 | OD2 | Average | Corrected |
50 | 2.432 | 2.389 | 2.411 | 2.265 |
25 | 1.822 | 1.831 | 1.827 | 1.681 |
12.5 | 1.276 | 1.281 | 1.279 | 1.133 |
6.25 | 0.819 | 0.804 | 0.812 | 0.666 |
3.12 | 0.431 | 0.434 | 0.433 | 0.287 |
1.56 | 0.234 | 0.243 | 0.239 | 0.093 |
0.78 | 0.189 | 0.184 | 0.187 | 0.041 |
0 | 0.149 | 0.142 | 0.146 | |
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Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
