PRSS2 Recombinant Monoclonal Antibody

Code CSB-RA789332A0HU
Size US$210
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  • Immunofluorescence staining of Jurkat cell with CSB-RA789332A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 562-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details

Uniprot No.
Target Names
PRSS2
Alternative Names
Trypsin-2 (EC 3.4.21.4) (Anionic trypsinogen) (Serine protease 2) (Trypsin II), PRSS2, TRY2 TRYP2
Species Reactivity
Human
Immunogen
A synthesized peptide derived from human PRSS2
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone No.
12H7
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Liquid
Tested Applications
ELISA, IF
Recommended Dilution
Application Recommended Dilution
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

The production of the PRSS2 recombinant monoclonal antibody involves genetic engineering techniques such as gene cloning and expression to obtain the gene that encodes for the PRSS2 monoclonal antibody. The synthesized peptide derived from human PRSS2 protein was used as an immunogen to generate the PRSS2 monoclonal antibody. Affinity chromatography purification ensures the high purity of the obtained PRSS2 recombinant monoclonal antibody, which specifically recognizes and binds to the human PRSS2 protein. The PRSS2 recombinant monoclonal antibody has been validated for use in ELISA and IF applications, which demonstrated the quality and specificity of the PRSS2 recombinant monoclonal antibody.

PRSS2 is a protease enzyme that is produced and secreted by the pancreas into the small intestine. It is a precursor of the active form of the enzyme, trypsin, which plays an essential role in the digestion of proteins. In the small intestine, PRSS2 is cleaved by an enzyme called enteropeptidase to generate trypsin, which in turn activates other pancreatic enzymes involved in protein digestion. Therefore, PRSS2 is an important component of the digestive system.

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Target Background

Function
In the ileum, may be involved in defensin processing, including DEFA5.
Gene References into Functions
  1. evaluated the association of claudin2 and PRSS1-PRSS2 polymorphisms with idiopathic recurrent acute (RAP) and chronic pancreatitis PMID: 26110235
  2. PRSS2 single nucleotide polymorphism associated with the development of alcoholic pancreatitis. PMID: 25253127
  3. Serum trypsinogen-2 is associated with pancreatic cancer and pancreatitis. PMID: 25916077
  4. trypsin-2 over-expression enhanced tongue carcinoma cell invasion by various genetic and proteolytic mechanisms. PMID: 22909050
  5. Elevation of urine trypsinogen 2 is an independent risk factor for pancreatic fistula after pancreaticoduodenectomy. PMID: 22357509
  6. Report a simple, rapid, easy, and noninvasive urinary trypsinogen-2 test can diagnose or rule out most cases of acute pancreatitis. PMID: 22481290
  7. Data show that urinary trypsinogen-2 dipstick test is a useful test for early diagnosis of post-endoscopic retrograde cholangiopancreatography (ERCP) pancreatitis. PMID: 21946810
  8. results suggest that trypsinogen-2 is a more sensitive marker than amylase, and it can be useful in early diagnosis of post-endoscopic retrograde cholangiopancreatography (ERCP) pancreatitis. PMID: 21792081
  9. A genetic variant of PRSS2 may contribute to the low prevalence of pancreatitis in primary hyperparathyroidism patients. PMID: 20625975
  10. Hyperexpression of the PRSS2 gene is associated with pancreatic cancer. PMID: 20428826
  11. there was a borderline statistically significant association between pancreatic cancer and HAT/HCT but no association between pancreatic cancer and HAT, HCT. PMID: 20484960
  12. Urine trypsinogen-2 test may be an important diagnostic tool in excluding the diagnosis of acute pancreatitis; it has a negative predictive value. PMID: 20517765
  13. Results do not support the urinary trypsinogen-2 dipstick test (UTDT) to replace standard plasma amylase for the diagnosis of AP, however, the test demonstrated an adequate sensitivity to be used for rapid early screening of AP in daily clinics. PMID: 19752771
  14. Results indicate that up-regulation of anionic trypsinogen in pancreatic diseases does not affect physiological trypsinogen activation, but significantly limits trypsin generation under potential pathological conditions. PMID: 12709065
  15. High levels of pulmonary trypsin-2 may be associated with the development of bronchopulmonary dysplasia in preterm infants. PMID: 12949286
  16. G191R variant of PRSS2 mitigates intrapancreatic trypsin activity and thereby protects against chronic pancreatitis PMID: 16699518
  17. Our results suggest that serum trypsinogen-2 is a most useful marker for diagnosing patients with cholangiocarcinoma, and it is superior to serum CA19-9 and CEA. PMID: 17640760
  18. Co-localization of trypsin-2 and MMP-9 resulted in intracellular proMMP-9 processing that represented fully or partially activated MMP-9. PMID: 18062964
  19. A loss of function polymorphism (G191R) of anionic trypsinogen (PRSS2) confers protection against chronic pancreatitis. PMID: 18362849
  20. A fusion with PRSS1 is associated with hereditary pancreatitis. PMID: 18461367
  21. primary role of trypsinogen sulfation in humans is to stimulate autoactivation of PRSS1 PMID: 18986305
  22. The p.G191R variant protected against alcoholic and idiopathic chronic pancreatitis as well as alcoholic acute pancreatitis in Japan. PMID: 19052022
  23. G191R PRSS2 is a rare allele in the Indian population and the data suggest a nonsignificant trend towards a protective effect from tropical calcific pancreatitis in Indians. PMID: 19077465

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Subcellular Location
Secreted, extracellular space.
Protein Families
Peptidase S1 family
Tissue Specificity
Expressed in Paneth cells, at the base of small intestinal crypts.
Database Links

HGNC: 9483

OMIM: 601564

KEGG: hsa:5645

UniGene: Hs.449281

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