Human Tumor susceptibility gene 101 protein(TSG101) ELISA kit

Code CSB-EL025125HU
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q99816
Lead Time 5-7 working days
Abbreviation TSG101
Protein Biological Process 1 Cell Cycle
Target Name tumor susceptibility gene 101
Alias TSG10, VPS23, tumor susceptibility protein
Species Homo sapiens (Human)
Protein Biological Process 3 Cell cycle
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 23.44 pg/mL-1500 pg/mL
Sensitivity 5.86 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression.
HGNC 15971
RGD 3909
MGI 106581
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human TSG101 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 98
Range % 92-104
1:2 Average % 108
Range % 103-112
1:4 Average % 94
Range % 90-98
1:8 Average % 89
Range % 86-95
Recovery
The recovery of human TSG101 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 102 99-106
EDTA plasma (n=4) 94 90-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1500 1.872 1.921 1.897 1.787
750 1.322 1.388 1.355 1.245
375 0.954 0.902 0.928 0.818
187.5 0.608 0.622 0.615 0.505
93.75 0.417 0.412 0.415 0.305
46.88 0.289 0.287 0.288 0.178
23.44 0.230 0.244 0.237 0.127
0 0.109 0.111 0.110  
.
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Function Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses. Required for the exosomal release of SDCBP, CD63 and syndecan
Subcellular Location Cytoplasm, Early endosome membrane, Peripheral membrane protein, Cytoplasmic side, Late endosome membrane, Peripheral membrane protein, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Midbody, Midbody ring, Nucleus
Protein Families Ubiquitin-conjugating enzyme family, UEV subfamily
Tissue Specificity Heart, brain, placenta, lung, liver, skeletal, kidney and pancreas.
Database Links

HGNC: 15971

OMIM: 601387

KEGG: hsa:7251

STRING: 9606.ENSP00000251968

UniGene: Hs.523512

Pathway Endocytosis
Secreted Extracellular Vesicles

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