Human Vascular Endothelial Growth Factor 121(VEGF121) ELISA kit

Instructions
Code CSB-E13709h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Vascular Endothelial Growth Factor 121(VEGF121)
Abbreviation VEGF121
Uniprot No. P15692
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human VEGF121 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %98
Range %92-106
1:2Average %94
Range %90-98
1:4Average %89
Range %86-96
1:8Average %92
Range %86-97
Recovery
The recovery of human VEGF121 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9894-104
EDTA plasma (n=4)9187-95
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
102.415 2.314 2.365 2.222
51.532 1.633 1.583 1.440
2.50.972 0.951 0.962 0.819
1.250.558 0.547 0.553 0.410
0.6250.404 0.411 0.408 0.265
0.3120.285 0.274 0.280 0.137
0.1560.226 0.220 0.223 0.080
00.143 0.142 0.143  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity).
Involvement in disease Microvascular complications of diabetes 1 (MVCD1)
Subcellular Location Secreted
Protein Families PDGF/VEGF growth factor family
Tissue Specificity Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed. A higher level expression seen in pituitary tumors as compared to the pituitary gland.
Database Links

HGNC: 12680

OMIM: 192240

KEGG: hsa:7422

STRING: 9606.ENSP00000361125

UniGene: Hs.73793

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