Human Vascular endothelial cell growth factor receptor 2,VEGFR-2/Flk-1 ELISA kit

Code CSB-E04763h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P35968
Lead Time 3-5 working days
Abbreviation VEGFR2
Protein Biological Process 1 Cytokine
Target Name kinase insert domain receptor (a type III receptor tyrosine kinase)
Alias CD309, FLK1, VEGFR, VEGFR2, fetal liver kinase-1|kinase insert domain receptor|protein-tyrosine kinase receptor Flk-1|soluble VEGFR2|tyrosine kinase growth factor receptor|vascular endothelial growt
Species Homo sapiens (Human)
Protein Biological Process 3 Angiogenesis
Sample Types serum, plasma, tissue homogenates
Detection Range 0.39 ng/mL-25 ng/mL
Sensitivity 0.098 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas.
HGNC 6307
RGD 2965
MGI 96683
Precision

 

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

 

Linearity

 

To assess the linearity of the assay, samples were spiked with high concentrations of human VEGFR-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

 

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

References
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Function Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain, such as isoform 2 and isoform 3, may function as decoy receptors for VEGFA, VEGFC and/or VEGFD. Isoform 2 plays an important role as negative regulator of VEGFA- and VEGFC-mediated lymphangiogenesis by limiting the amount of free VEGFA and/or VEGFC and preventing their binding to FLT4. Modulates FLT1 and FLT4 signaling by forming heterodimers. Binding of vascular growth factors to isoform 1 leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, reorganization of the actin cytoskeleton and activation of PTK2/FAK1. Required for VEGFA-mediated induction of NOS2 and NOS3, leading to the production of the signaling molecule nitric oxide (NO) by endothelial cells. Phosphorylates PLCG1. Promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1 and SRC.
Involvement in disease Hemangioma, capillary infantile (HCI)
Subcellular Location Cell junction, Endoplasmic reticulum, Note=Localized with RAP1A at cell-cell junctions (By similarity), Colocalizes with ERN1 and XBP1 in the endoplasmic reticulum in endothelial cells in a vascular endothelial growth factor (VEGF)-dependent manner (PubMed:23529610), SUBCELLULAR LOCATION: Isoform 1: Cell membrane, Single-pass type I membrane protein, Cytoplasm, Nucleus, Cytoplasmic vesicle, Early endosome
Protein Families Protein kinase superfamily, Tyr protein kinase family, CSF-1/PDGF receptor subfamily
Tissue Specificity Detected in cornea (at protein level). Widely expressed.
Database Links

HGNC: 6307

OMIM: 191306

KEGG: hsa:3791

STRING: 9606.ENSP00000263923

UniGene: Hs.479756

Pathway MAPK signaling pathway
PI3K-Akt signaling pathway
VEGF signaling pathway
Focal adhesion
Ras signaling pathway
Rap1 signaling pathway

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